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Src and Cas are essentially but differentially involved in angiotensin II-stimulated migration of vascular smooth muscle cells via extracellular signal-regulated kinase 1/2 and c-Jun NH2-terminal kinase activation

Angiotensin II (Ang II) plays an important role in several cardiovascular diseases associated with vascular smooth muscle cell (VSMC) growth and migration. Src activity is known to be required for the migration of a number of cell types. p130Cas was...
Authors: Kyaw M, Yoshizumi M, Tsuchiya K, Kagami S, Izawa Y, Fujita Y, Ali N, Kanematsu Y, Toida K, Ishimura...

Efficient gene transfer into the human natural killer cell line, NKL, using the amaxa nucleofection system

Natural killer (NK) cell lines are useful for studying facets of NK cell biology. Such cell lines are notoriously difficult to transfect by traditional methods, a fact that has hampered NK cell biology studies for a long time. To overcome this, we...
Authors: Maasho K, Marusina A, Reynolds NM, Coligan JE and Borrego F

Human mesenchymal stem cells as a gene delivery system to create cardiac pacemakers

We tested the ability of human mesenchymal stem cells (hMSCs) to deliver a biological pacemaker to the heart. hMSCs transfected with a cardiac pacemaker gene, mHCN2, by electroporation expressed high levels of Cs+-sensitive current (31.1+/-3.8 pA/pF...
Authors: Potapova I, Plotnikov A, Lu Z, Danilo P Jr, Valiunas V, Qu J, Doronin S, Zuckerman J, Shlapakova IN,...

Nucleofection as an efficient nonviral transfection method for human monocytic cells

Despite some progress in the field of gene transfer into hard-to-transfect cells, so far an efficient nonviral method for monocytes has not been available. A comparison of plasmid DNA with capped and polyadenylated mRNA for enhanced green fluorescent...
Authors: Martinet W, Schrijvers DM and Kockx MM

LINGO-1 is a component of the Nogo-66 receptor/p75 signaling complex

Axon regeneration in the adult CNS is prevented by inhibitors in myelin. These inhibitors seem to modulate RhoA activity by binding to a receptor complex comprising a ligand-binding subunit (the Nogo-66 receptor NgR1) and a signal transducing subunit...
Authors: Mi S, Lee X, Shao Z, Thill G, Ji B, Relton J, Levesque M, Allaire N, Perrin S, Sands B, Crowell T,...

Marlin-1 a novel RNA binding protein associates with GABA(subB) receptors

GABA(subB) receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the CNS. While heterodimerization between GABA(subB) receptor GABA(subB)R1 and GABA(subB)R2 subunits is essential for functional expression,...
Authors: Couve A, Restituito S, Brandon JM, Charles KJ, Bawagan H, Freeman KB, Pangalos MN, Calver AR and...

CD154 transcriptional regulation in primary human CD4 T cells

CD154 (CD40-ligand) has a wide variety of pleiotropic effects throughout the immune system and is critical to both cellular and humoral immunity. Cell surface and soluble CD154 are primarily expressed by activated CD4 T cells. Expression of CD154 is...
Authors: Cron RQ

Ligand-independent redistribution of Fas (CD95) into lipid rafts mediates clonotypic T cell death

Clonotypic elimination of activated T cells through Fas-Fas ligand (CD95-CD95L) interactions is one mechanism of peripheral self-tolerance. T cell receptor (TCR) stimuli trigger FasL synthesis but also sensitize activated T cells to Fas-mediated...
Authors: Muppidi JR and Siegel RM

Neuron-derived orphan receptor-1 (NOR-1) modulates vascular smooth muscle cell proliferation

Vascular smooth muscle cells (VSMCs) migration and proliferation play a key role in the pathophysiology of cardiovascular disease. However, the transcription factors that regulate VSMC activation are not completely characterized. By a...
Authors: Martinez-Gonzalez J, Rius J, Castellܳ A, Cases-Langhoff C and Badimon L

Rapid and efficient electroporation-based gene transfer into primary dissociated neurons

Non-viral gene transfer into neurons has proved to be a formidable task. Here, we describe an electroporation-based method that allows efficient and reliable DNA transfer into dissociated neural cells before they are plated and cultured. In...
Authors: Dityateva G, Hammond M, Thiel C, Ruonala MO, Delling M, Siebenkotten G, Nix M and Dityatev A
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