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Nucleofection of primary neurons

Efficient gene transfer is an important tool for the study of neuronal function and biology. This has proved difficult and inefficient with traditional transfection strategies, which can also be fairly toxic, whereas viral-mediated gene transfer,...

Authors: Gartner A, Collin L, Lalli G

Activation or suppression of NFkappaB by HPK1 determines sensitivity to activation-induced cell death

Restimulation of the T-cell receptor (TCR) in activated T cells induces CD95 (Fas/Apo-1)-mediated activation-induced cell death (AICD). The TCR-proximal mechanisms leading to AICD are elusive. Here we characterize hematopoietic progenitor kinase 1...

Authors: Brenner D, Golks A, Kiefer F, Krammer PH and Arnold R

Urea flux across MDCK-mUT-A2 monolayers is acutely sensitive to AVP, cAMP and [Ca2+]i

In this study we engineered an MDCK type I cell line to stably express the mouse urea transporter UT-A2. Monolayers of MDCK-mUT-A2 cells had a basal phloretin-inhibitable urea permeability of 8.4x10(-6) +/- 0.3 cm/sec. Treatment of MDCK-mUT-A2...

Authors: Potter EA, Stewert G, Smith CP

The GRIP1:IRF3 interaction as a target for glucocorticoid receptor-mediated immunosuppression

Glucocorticoids dramatically inhibit cytokine and chemokine production. They act through the glucocorticoid receptor (GR), a ligand-dependent transcription factor that binds to and represses activities of other DNA-bound regulators, activator protein...

Authors: Reily MM, Pantoja C, Hu X, Chinenov Y and Rogatsky I

Cdk5 phosphorylates and stabilizes p27(kip1) contributing to actin organization and cortical neuronal migration

p27(kip1), a cyclin-dependent kinase (CDK) inhibitor (CKI), generally suppresses CDK activity in proliferating cells. Although another role of p27 in cell migration has been recently suggested in vitro, the physiological importance of p27 in cell...

Authors: Kawauchi T, Chihama K, Nabeshima Y and Hoshino M

A schizophrenia-associated mutation of DISC1 perturbs cerebral cortex development

Disrupted-In-Schizophrenia-1 (DISC1), originally identified at the breakpoint of a chromosomal translocation that is linked to a rare familial schizophrenia, has been genetically implicated in schizophrenia in other populations. Schizophrenia...

Authors: Kamiya A, Kubo K, Tomoda T, Takaki M, Youn R, Ozeki Y, Sawamura N, Park U, Kudo C, Okawa M, Ross CA,...

Posttranslational Regulation of Tristetraprolin Subcellular Localization and Protein Stability by p38 Mitogen-Activated Protein Kinase and Extracellular Signal-Regulated Kinase Pathways

The p38 mitogen-activated protein kinase (MAPK) signaling pathway, acting through the downstream kinase MK2, regulates the stability of many proinflammatory mRNAs that contain adenosine/uridine-rich elements (AREs). It is thought to do this by...

Authors: Brook M, Tchen CR, Santalucia T, McIlrath J, Arthur JS, Saklatvala J, Clark AR

Characterization of CD 200-receptor expression in the murine epidermis

CD 200 is a widely expressed transmembrane glycoprotein that transmits an inhibitory signal after ligation of the structurally homologous CD 200-receptor-1 (CD 200 R1). Recently, we showed that CD 200 is expressed on keratinocytes and plays a role in...

Authors: Rosenblum MD, Woodliff JE, Madsen NA, McOlash LJ, Keller MR and Truitt RL

Epigenetic processes play a major role in B-cell-specific gene silencing in classical Hodgkin lymphoma

Many B-lineage-specific genes are down-regulated in Hodgkin and Reed-Sternberg (HRS) cells of classical Hodgkin lymphoma (cHL). We investigated the involvement of epigenetic modifications in gene silencing in cHL cell lines and in microdissected...

Authors: Ushmorov A, Leithauser F, Sakk O, Weinhausel A, Popov SW, Moller P, Wirth T

Varicella-Zoster Virus ORF63 Inhibits Apoptosis of Primary Human Neurons

Virus-encoded modulation of apoptosis may serve as a mechanism to enhance cell survival and virus persistence. The impact of productive varicella-zoster virus (VZV) infection on apoptosis appears to be cell type specific, as infected human sensory...

Authors: Hood C, Cunningham AL, Slobedman B, Arvin AM, Sommer MH, Kinchington PR and Abendroth A

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