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Legionella subvert the functions of rab1 and sec22b to create a replicative organelle

Legionella pneumophila is a bacterial pathogen that infects eukaryotic host cells and replicates inside a specialized organelle that is morphologically similar to the endoplasmic reticulum (ER). To better understand the molecular mechanisms governing...

Authors: Kagan JC, Stein MP, Pypaert M and Roy CR

A missense single-nucleotide polymorphism in a gene encoding a protein tyrosine phosphatase (PTPN22) is associated with rheumatoid arthritis

Rheumatoid arthritis (RA) is the most common systemic autoimmune disease, affecting approximately 1% of the adult population worldwide, with an estimated heritability of 60%. To identify genes involved in RA susceptibility, we investigated the...

Authors: Begovich AB, Carlton VE, Honigberg LA, Schrodi SJ, Chokkalingam AP, Alexander HC, Ardlie KG, Huang...

Chromatin remodeling and histone modification in the conversion of oligodendrocyte precursors to neural stem cells

We showed previously that purified rat oligodendrocyte precursor cells (OPCs) can be induced by extracellular signals to convert to multipotent neural stem-like cells (NSLCs), which can then generate both neurons and glial cells. Because the...

Authors: Kondo T and Raff M

Reconstitution of deficient T cell receptor zeta chain restores T cell signaling and 8ments T cell receptor/CD3-induced interleukin-2 production in patients with systemic lupus erythematosus

OBJECTIVE: T cells from a majority of patients with systemic lupus erythematosus (SLE) display antigen receptor-mediated signaling aberrations associated with defective T cell receptor (TCR) zeta chain, a subunit of the TCR/CD3 complex. This study...

Authors: Nambiar MP, Fisher CU, Warke VG, Krishnan S, Mitchell JP, Delaney N and Tsokos GC

Photobleaching-Corrected FRET Efficiency Imaging of Live Cells

Fluorescent resonance energy transfer (FRET) imaging techniques can be used to visualize protein-protein interactions in real-time with subcellular resolution. Imaging of sensitized fluorescence of the acceptor, elicited during excitation of the...

Authors: Zal T and Gascoigne NR

Reversible oxidation and inactivation of the tumor suppressor PTEN in cells stimulated with peptide growth factors

Stimulation of cells with various peptide growth factors induces the production of phosphatidylinositol 3,4,5-trisphosphate (PIP3) through activation of phosphatidylinositol 3-kinase. The action of this enzyme is reversed by that of the tumor...

Authors: Kwon J, Lee SR, Yang KS, Ahn Y, Kim YJ, Stadtman ER and Rhee SG

Mechanism of NF-kappaB inactivation induced by survival signal withdrawal in cerebellar granule neurons

Activity of the transcription factor nuclear factor-kappaB (NF-kappaB) has been shown to be necessary for maintaining neuronal viability. In cultured rat cerebellar granule neurons, trophic factor withdrawal induces NF-kappaB inactivation, resulting...

Authors: Kovacs AD, Chakraborty-Sett S, Ramirez SH, Sniderhan LF, Williamson AL and Maggirwar SB

PTEN regulates motility but not directionality during leukocyte chemotaxis

The localization at opposite cell poles of phosphatidylinositol-3 kinases and PTEN (phosphatase and tensin homolog on chromosome 10) governs Dictyostelium chemotaxis. To study this model in mammalian cells, we analyzed the dynamic redistribution of...

Authors: Lacalle RA, Gomez-Mouton C, Barber DF, Jimenez-Baranda S, Mira E, Martinez-A C, Carrera AC, Manes S

Novel non-viral method for transfection of primary leukemia cells and cell lines

BACKGROUND: Tumor cells such as leukemia and lymphoma cells are possible targets for gene therapy. However, previously leukemia and lymphoma cells have been demonstrated to be resistant to most of non-viral gene transfer methods. METHODS: The aim of...

Authors: Schakowski F, Buttgereit P, Mazur M, Marten A, Schottker B, Gorschluter M and Schmidt-Wolf IG

Dendritic cell, the immunotherapeutic cell for cancer

Dendritic cells play an important role in the development of effective cancer vaccines. These cells have the potential to present tumour-specific antigens and thereby induce an immune response. Various studies involving clinical trials have...

Authors: Nagaraj S, Ziske C and Schmidt-Wolf IGH

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