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Differential localization and function of ADP-ribosylation factor-6 in anergic human T cells: a potential marker for their identification

Anergy is a state of immunologic tolerance in which T cells are viable but incapable of responding to antigenic stimulation. Recent data indicate that anergic cells have a distinct gene expression program that determines their unique function. In...

Authors: Tzachanis D, Appleman LJ, van Puijenbroek AAFL, Berezovskaya A, Nadler LM and Boussiotis VA

Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase A inhibits T cell receptor signaling

Raft-associated Csk controls signaling through the T cell receptor (TCR) and was mainly anchored to Cbp/PAG (phosphoprotein associated with glycosphingolipid-enriched membrane domains). Treatment of cells with the cAMP-elevating agent prostaglandin...

Authors: Vang T, Abrahamsen H, Myklebust S, Horejesi V and Taskén K

IL-10-inducible Bcl-3 negatively regulates LPS-induced TNF-alpha production in macrophages

Interleukin-10 (IL-10) plays an important role in prevention of chronic inflammation in vivo. However, the molecular mechanism by which IL-10 exerts its anti-inflammatory response is poorly understood. Here, we performed a microarray analysis and...

Authors: Kuwata H, Watanabe Y, Miyoshi H, Yamamoto M, Kaisho T, Takeda K and Akira S

A central role for the Jun-N-terminal kinase pathway in mediating the antagonistic activity of pro-inflammatory cytokines against transforming growth factor-beta-driven Smad3/4 specific gene expression

We have focused our attention on the molecular events underlying the antagonistic activities of pro-inflammatory cytokines against transforming growth factor-beta (TGF-beta)/SMAD signaling. Using jnk1/2-knockout (jnk(-/-)) and I kappa B...

Authors: Verrecchia F, Tacheau C, Wagner EF and Mauviel A

Distinct involvement of the Jun-N-terminal kinase and NF-kappaB pathways in the expression of the human COL1A2 gene by TNF-alpha

We used a gene knockout approach to elucidate the specific roles played by the Jun-N-terminal kinase (JNK) and NF-kappaB pathways downstream of TNF-alpha in the context of alpha(2) type I collagen gene (COL1A2) expression. In JNK1-/--JNK2-/- (JNK-/-)...

Authors: Verrecchia F, Wagner EF and Mauviel A

Coordinate notch 3-hairy related transscription factor pathway regulation in response to arterial injury: mediator role of PDGF and ERK

The Notch family of receptors and downstream effectors plays a critical role in cell fate determination during vascular ontogeny. Moreover, the human cerebral autosomal dominant artriopathy with subcortical infarcts and leukoencephalopathy (CADASIL)...

Authors: Wang W, Campos AH, Prince CZ, Mou Y and Pollmann MJ

Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells

Dendritic cells (DCs) are ideal accessory cells in the developing field of gene therapy. Although viral transfection of DCs has become widespread, non-viral transfection of DCs has shown disappointing results. Recently, a new technique for...

Authors: Lenz P, Bacot SM, Frazier-Jessen MR and Feldman GM

Interactions of human myosin Va isoforms, endogenously expressed in human melanocytes, are tightly regulated by the tail domain

Primary human epidermal melanocytes express six endogenous isoforms of the human actin-associated myosin Va motor protein, involved in organelle transport. As isoforms containing exon F are most abundant in melanocytes, we hypothesized that these...

Authors: Westbroek W, Lambert J, Bahadoran P, Busca R, Herteleer MC, Smit N, Mommaas M, Ballotti R and...

Shear stress-induced endothelial cell polarization in mediated by Rho and Rac but not Cdc42 or PI 3-kinase

Shear stress induces endothelial polarization and migration in the direction of flow accompanied by extensive remodeling of the actin cytoskeleton. The GTPases RhoA, Rac1, and Cdc42 are known to regulate cell shape changes through effects on the...

Authors: Wojciak-Stothard B and Ridley AJ

Proteasome inhibition results in TRAIL sensitization of primary keratinocytes by removing the restistance-mediating block of effector caspase maturation

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) exerts potent cytotoxic activity against transformed keratinocytes, whereas primary keratinocytes are relatively resistant. In several cell types, inhibition of the proteasome sensitizes...

Authors: Leverkus M, Sprick MR, Wachter T, Mengling T, Baumann B, Serfling E, Brocker EB, Goebeler M, Neumann...

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