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NPC1 and NPC2 regulate cholesterol homeostasis through generation of LDL cholesterol-derived oxysterols

Mutations in the Niemann-Pick disease genes cause lysosomal cholesterol accumulation and impaired low density lipoprotein (LDL) cholesterol esterification. These findings have been attributed to a block in cholesterol movement from lysosomes to the...

Authors: Frolov A, Zielinski SE, Crowley JR, Dudley-Rucker N, Schaffer JE and Ory DS

High-resolution multicolor imaging of dynamic signaling complexes in T cells stimulated by planar substrates

The dynamic visualization of developing immunological synapses has been hindered by the difficulty of imaging the contact between the T cell and the antigen-presenting cell (APC). Here, we describe a technique in which T cell responses are...

Authors: Bunnell SC, Barr VA, Fuller CL and Samelson LE

The gene product Murr1 restricts HIV-1 replication in resting CD4+ lymphocytes

Although human immunodeficiency virus-1 (HIV-1) infects quiescent and proliferating CD4+ lymphocytes, the virus replicates poorly in resting T cells. Factors that block viral replication in these cells might help to prolong the asymptomatic phase of...

Authors: Ganesh L, Burstein E, Guha-Niyogi A, Louder MK, Mascola JR, Klomp LW, Wijmenga C, Duckett CS and...

T cell receptor ligation induces the formation of dynamically regulated signaling assemblies.

Tcell antigen receptor (TCR) ligation initiates tyrosine kinase activation, signaling complex assembly, and immune synapse formation. Here, we studied the kinetics and mechanics of signaling complex formation in live Jurkat leukemic T cells using...

Authors: Bunnell SC, Hong DI, Kardon JR, Yamazaki T, McGlade CJ, Barr VA and Samelson LE

Evidence that high teleromerase activity may induce a senescent-like growth arrest in human fibroblasts

Expression of the catalytic subunit of human telomerase (hTERT), in normal human fibroblasts allows them to escape replicative senescence. However, we have observed that populations of hTERT-immortalized human fibroblasts contain 3-20% cells with a...

Authors: Gorbunova V, Seluanov A and Pereira-Smith OM

Efficient transfection method for primary cells

Transfection of primary cells and stem cells is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells. Here we describe the...

Authors: Hamm A, Krott N, Breibach I, Blindt R and Bosserhoff AK

Determinants of Notch-3 receptor expression and signaling in vascular smooth muscle cells. Implications in cell-cycle regulation

The Notch family of receptors and ligands plays an important role in cell fate determination, vasculogenesis, and organogenesis. Mutations of the Notch-3 receptor result in an arteriopathy that predisposes to early-onset stroke. However, the...

Authors: Campos AH, Wang W, Pollmann MJ and Gibbons GH

Weibel-Palade bodies recruit Rab27 by a content-driven, maturation-dependent mechanism that is independent of cell type

The identification of organelles is crucial for efficient cellular function, yet the basic underlying mechanisms by which this might occur have not been established. One group of proteins likely to be central to organelle identity is the Rab family...

Authors: Hannah MJ, Hume AN, Arribas M, Williams R, Hewlett LJ, Seabra MC and Cutler DF

HSP27 inhibits release of mitochondrial protein Smac in multiple myeloma cells and confers dexamethasone resistance

Smac, second mitochondria-derived activator of caspases, promotes apoptosis via activation of caspases. Heat shock protein 27 (Hsp27) negatively regulates another mitochondrial protein, cytochrome c, during apoptosis; however, the role of Hsp27 in...

Authors: Chauhan D, Li G, Hideshima T, Podar K, Mitsiades C, Midsiades N, Catley L, Tai YT, Hayashi T,...

Imaging antigen-induced PI3K activation in T cells

Imaging antigen-induced PI3K activation in T cells. Harriague J, Bismuth G. Departement de Biologie Cellulaire, Institut Cochin, INSERM U567, CNRS UMR 7627, Universite Rene Descartes, 22 rue Mechain, 75014 Paris, France. Activation of...

Authors: Harriague J and Bismuth G

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