Search Results

RNA-Seq analysis to capture the transcriptome landscape of a single cell.

We describe here a protocol for digital transcriptome analysis in a single mouse oocyte and blastomere using a deep-sequencing approach. In this method, individual cells are isolated and transferred into lysate buffer by mouth pipette, followed by...

Authors: Tang F1, Barbacioru C, Nordman E, Li B, Xu N, Bashkirov VI, Lao K, Surani MA.

Selective association of peroxiredoxin 1 with genomic DNA and COX-2 upstream promoter elements in estrogen receptor negative breast cancer cells.

In a search for proteins differentially cross-linked to DNA by cisplatin or formaldehyde in normal breast epithelial and breast cancer cell lines, we identified peroxiredoxin 1 (PRDX1) as a protein preferentially cross-linked to DNA in estrogen...

Authors: Wang X1, He S, Sun JM, Delcuve GP, Davie JR.

Overexpression of sICAM-1 in the alveolar epithelial space results in an exaggerated inflammatory response and early death in Gram negative pneumonia

BACKGROUND: A sizeable body of data demonstrates that membrane ICAM-1 (mICAM-1) plays a significant role in host defense in a site-specific fashion. On the pulmonary vascular endothelium, mICAM-1 is necessary for normal leukocyte recruitment...

Authors: Mendez MP, Monroy YK, Du M, Preston AM, Tolle L, Lin Y, VanDussen KL, Samuelson LC, Standiford TJ,...

Prostaglandin (PG) F2 alpha synthesis in human subcutaneous and omental adipose tissue: modulation by inflammatory cytokines and role of the human aldose reductase AKR1B1

INTRODUCTION: PGF2a may be involved in the regulation of adipose tissue function. OBJECTIVES: 1) To examine PGF2a release by primary preadipocytes, mature adipocytes and whole tissue explants from the subcutaneous and omental fat compartments; 2)...

Authors: Michaud A1, Lacroix-Pépin N2, Pelletier M3, Veilleux A4, Noël S5, Bouchard C5, Marceau P6, Fortier...

Rapid and reliable detection of a-globin copy number variations by quantitative real-time PCR

BACKGROUND: Alpha-thalassemia is the most common human genetic disease worldwide. Copy number variations in the form of deletions of a-globin genes lead to a-thalassemia while duplications of a-globin genes can cause a severe phenotype in...

Authors: Grimholt RM, Urdal P, Klingenberg O, Piehler AP

CCN2 exerts direct cytoprotective actions in adult cardiac myocytes by activation of the PI3-kinase/Akt/GSK-3ß signaling pathway.

We recently reported that transgenic mice with cardiac-restricted overexpression of CCN2/CTGF have substantially increased tolerance towards ischemia/reperfusion injury. The purpose of this study was to investigate to what extent fully differentiated...

Authors: Moe IT1, Pham TA, Hagelin EM, Ahmed MS, Attramadal H

Heat shock protein 90 inhibitors: new mode of therapy to overcome endocrine resistance

Aromatase inhibitors are important drugs to treat estrogen receptor alpha (ERalpha)-positive postmenopausal breast cancer patients. However, development of resistance to aromatase inhibitors has been observed. We examined whether the heat shock...

Authors: Wong C1, Chen S.

Regulation of NADPH oxidase 5 by protein kinase C isoforms.

NADPH oxidase5 (Nox5) is a novel Nox isoform which has recently been recognized as having important roles in the pathogenesis of coronary artery disease, acute myocardial infarction, fetal ventricular septal defect and cancer. The activity of Nox5...

Authors: Chen F, Yu Y, Haigh S, Johnson J, Lucas R, Stepp DW, Fulton DJ.

Wnt signaling activation and mammary gland hyperplasia in MMTV-LRP6 transgenic mice: implication for breast cancer tumorigenesis.

Although Wnt signaling activation is frequently observed in human breast cancer, mutations in genes encoding intracellular components of the Wnt signaling pathway are rare. We found that the expression of Wnt signaling co-receptor, LRP6, is...

Authors: Zhang J1, Li Y, Liu Q, Lu W, Bu G.

Combining chemotherapeutic agents and netrin-1 interference potentiates cancer cell death.

The secreted factor netrin-1 is upregulated in a fraction of human cancers as a mechanism to block apoptosis induced by netrin-1 dependence receptors DCC and UNC5H. Targeted therapies aiming to trigger tumour cell death via netrin-1/receptors...

Authors: Paradisi A, Creveaux M, Gibert B, Devailly G, Redoulez E, Neves D, Cleyssac E, Treilleux I, Klein C,...

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