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4916 results sorted by

Impact of SRC homology 2-containing inositol 5'-phosphatase 2 gene polymorphisms detected in a Japanese population on insulin signaling

Src homology 2-containing 5'-inositol phosphatase 2 (SHIP2) is known to be one of lipid phosphatases converting PI(3,4,5)P3 to PI(3,4)P2 in the negative regulation of insulin signaling with the fundamental impact on the state of insulin resistance....
Authors: Kagawa S, Sasaoka T, Yaguchi S, Ishihara H, Tsuneki H, Murakami S, Fukui K, Wada T, Kobayashi S,...

mRNA but not plasmid DNA is efficiently transfected in murine J774A.1 macrophages

Previous studies demonstrated that macrophages are difficult to transfect. In the present study, we investigated whether J774A.1 macrophages can be efficiently transfected using nucleofector technology. Nucleofection of J774A.1 macrophages with mRNA...
Authors: Van De Parre TJ, Martinet W, Schrijvers DM, Herman AG and De Meyer GR

Lowe syndrome protein Ocrl1 is translocated to membrane ruffles upon Rac GTPase activation : a new perspective on Lowe syndrome pathophysiology

Oculocerebrorenal Lowe syndrome is a rare X -linked disorder characterized by bilateral cataract, mental retardation and renal Fanconi syndrome. The Lowe syndrome protein Ocrl1 is a PIP2 5-phosphatase, primarily localized to the trans-Golgi network...
Authors: Faucherre A, Desbois P, Nagano F, Satre V, Lunardi J, Gacon G and Dorseuil O

Human FK506 binding protein 65 is associated with colorectal cancer

We initiated the present study to identify new genes associated with colorectal cancer. In a previously published microarray study an EST (W80763), later identified as the gene hFKBP10 (NM_021939), was found to be strongly expressed in tumors while...
Authors: Olesen SH, Christensen LL, Sorensen FB, Cabezon T, Laurberg S, Orntoft TF and Birkenkamp-Demtroder...

Effective gene delivery to adult neurons by a modified form of electroporation

Non-viral methods of transfection of cDNAs into adult neurons and other post-mitotic cells are generally very inefficient. However, the recent development of Nucleofectortrade mark technology developed by Amaxa Biosystems allows direct delivery of...
Authors: Leclere PG, Panjwani A, Docherty R, Berry M, Pizzey J, Tonge DA

c-Jun NH2-terminal kinase-interacting protein-3 facilitates phosphorylation and controls localization of amyloid-beta precursor protein

Abnormal phosphorylation of amyloid-beta precursor protein (APP) is a pathologic feature of Alzheimer's disease. To begin to understand the mechanism of APP phosphorylation, we studied this process in differentiating neurons under normal...
Authors: Muresan Z and Muresan V

A local coupling model and compass parameter for eukaryotic chemotaxis

Chemotaxis is a cellular sensing mechanism that guides immune cells to sites of infection and leads fibroblasts to sites of injury. Here, we show in migrating primary dendritic cells and fibroblasts that the leading edge is not a uniform signaling...
Authors: Arrieumerlou C and Meyer T

Sphingosine kinase-1 enhances endothelial cell survival through a PECAM-1-dependent activation of PI-3K/Akt and regulation of Bcl-2 family members

SSphingosine-1-phosphate (S1P), the bioactive product of sphingosine kinase (SK) activation is a survival factor for endothelial cells. The mechanism of SK mediated survival was investigated in endothelial cells with moderately raised intracellular...
Authors: Limaye VS, Li X, Hahn C, Xia P, Berndt MC, Vadas MA and Gamble JR

Coronin-1 Function Is Required for Phagosome Formation

Coronin-1 is an actin-associated protein whose function in actin dynamics has remained obscure. All coronin proteins have a variable N terminal domain, followed by WD repeats and a C-terminal coiled-coil dimerization domain. Transfection of...
Authors: Yan M, Collins RF, Grinstein S and Trimble WS

Molecular interactions of B-CAM (basal-cell adhesion molecule) and laminin in epithelial skin cancer

Molecular events underlying the progression of malignant tumors through the surrounding tissue are largely mediated by membrane-bound adhesion molecules. Basal-cell adhesion molecule (B-CAM), a 90-kDa laminin receptor of the immunoglobulin...
Authors: Drewniok C, Wienrich BG, Schon M, Ulrich J, Zen Q, Telen MJ, Hartig RJ, Wieland I, Gollnick H and...
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