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Transient transfection of DT40

Transient transfection of cell lines can be extremely useful in a number of applications. However, until recently, obtaining useful and reproducible percentages of transiently expressing DT40 was almost impossible. Standard techniques such as...

Authors: Franklin R, Sale JE

Astrocytes regulate GluR2 expression in motor neurons and their vulnerability to excitotoxicity

Influx of Ca(2+) ions through alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptors contributes to neuronal damage in stroke, epilepsy, and neurodegenerative disorders such as ALS. The Ca(2+) permeability of AMPA receptors is...

Authors: Van Damme P, Bogaert E, Dewil M, Hersmus N, Kiraly D, Scheveneels W, Bockx I, Braeken D, Verpoorten...

Decreased expression of Kruppel-like factors in memory B cells induces the rapid response typical of secondary antibody responses

Secondary antibody responses are characterized by the rapid kinetics of the responding cells, including the production of larger amounts of serum Ig compared with the primary response. Memory B cells, which are responsible for this phenomenon,...

Authors: Good KL, Tangye SG

Essential role for Rap1 GTPase and its guanine exchange factor CalDAG-GEFI in LFA-1 but not VLA-4 integrin-mediated human T cell adhesion

Regulated adhesion of T cells by the integrins LFA-1 and VLA-4 is essential for T cell trafficking. The small GTPase Rap1 is a critical activator of both integrins in murine lymphocytes and T cell lines. Here we examined the contribution of the Rap1...

Authors: Ghandour H, Cullere X, Luscinskas FW, Mayadas TN

Robust CD4(+) and CD8(+) T cell responses to SIV using mRNA-transfected DC expressing autologous viral Ag

A potentially powerful strategy for therapeutic HIV vaccination is the use of DC transfected with mRNA encoding autologous viral Ag, as epitopes presented by transfected DC would exactly reflect those expressed by infected cells in the individual....

Authors: Melhem NM, Liu XD, Boczkowski D, Gilboa E, Barratt-Boyes SM

Retrograde Fluxes of Focal Adhesion Proteins in Response to Cell Migration and Mechanical Signals

Recent studies suggest that mechanical signals mediated by the extracellular matrix play an essential role in various physiological and pathological processes, yet how cells respond to mechanical stimuli remains elusive. Using live cell fluorescence...

Authors: Guo WH, Wang YL

Efficient and stable transgene expression in human embryonic stem cells using transposon-mediated gene transfer

Efficient and stable genetic modification of human embryonic stem (ES) cells is required to realize the full scientific and potential therapeutic use of these cells. Currently, only limited success toward this goal has been achieved without using a...

Authors: Wilber A, Linehan JL, Tian X, Woll PS, Morris JK, Belur LR, McIvor RS, Kaufman DS

Contributions of galectin-3 and -9 to epithelial cell adhesion analysed by single cell force spectroscopy

Galectins are widely expressed in epithelial tissues and have been implicated in a variety of cellular processes, including adhesion and polarization. Here we studied the contributions of galectins in cell adhesion and cyst formation of Madin-Darby...

Authors: Friedrichs J, Torkko JM, Helenius J, Teräväinen TP, Füllekrug J, Muller DJ, Simons K, Manninen A

Signal-Transducing Adaptor Protein-2 Regulates Integrin-Mediated T Cell Adhesion through Protein Degradation of Focal Adhesion Kinase

Signal-transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein that contains pleckstrin homology- and Src homology 2-like domains as well as a YXXQ motif in its C-terminal region. Our previous studies demonstrated that STAP-2...

Authors: Sekine Y, Tsuji S, Ikeda O, Sugiyma K, Oritani K, Shimoda K, Muromoto R, Ohbayashi N, Yoshimura A,...

FLASH assembly of TALENs for high-throughput genome editing.

Engineered transcription activator–like effector nucleases (TALENs) have shown promise as facile and broadly applicable genome editing tools. However, no publicly available high-throughput method for constructing TALENs has been published, and...

Authors: Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK.

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