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NPC1 and NPC2 regulate cholesterol homeostasis through generation of LDL cholesterol-derived oxysterols

Mutations in the Niemann-Pick disease genes cause lysosomal cholesterol accumulation and impaired low density lipoprotein (LDL) cholesterol esterification. These findings have been attributed to a block in cholesterol movement from lysosomes to the...

Authors: Frolov A, Zielinski SE, Crowley JR, Dudley-Rucker N, Schaffer JE and Ory DS

The gene product Murr1 restricts HIV-1 replication in resting CD4+ lymphocytes

Although human immunodeficiency virus-1 (HIV-1) infects quiescent and proliferating CD4+ lymphocytes, the virus replicates poorly in resting T cells. Factors that block viral replication in these cells might help to prolong the asymptomatic phase of...

Authors: Ganesh L, Burstein E, Guha-Niyogi A, Louder MK, Mascola JR, Klomp LW, Wijmenga C, Duckett CS and...

Evidence that high teleromerase activity may induce a senescent-like growth arrest in human fibroblasts

Expression of the catalytic subunit of human telomerase (hTERT), in normal human fibroblasts allows them to escape replicative senescence. However, we have observed that populations of hTERT-immortalized human fibroblasts contain 3-20% cells with a...

Authors: Gorbunova V, Seluanov A and Pereira-Smith OM

Efficient transfection method for primary cells

Transfection of primary cells and stem cells is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells. Here we describe the...

Authors: Hamm A, Krott N, Breibach I, Blindt R and Bosserhoff AK

Weibel-Palade bodies recruit Rab27 by a content-driven, maturation-dependent mechanism that is independent of cell type

The identification of organelles is crucial for efficient cellular function, yet the basic underlying mechanisms by which this might occur have not been established. One group of proteins likely to be central to organelle identity is the Rab family...

Authors: Hannah MJ, Hume AN, Arribas M, Williams R, Hewlett LJ, Seabra MC and Cutler DF

Imaging antigen-induced PI3K activation in T cells

Imaging antigen-induced PI3K activation in T cells. Harriague J, Bismuth G. Departement de Biologie Cellulaire, Institut Cochin, INSERM U567, CNRS UMR 7627, Universite Rene Descartes, 22 rue Mechain, 75014 Paris, France. Activation of...

Authors: Harriague J and Bismuth G

Regulation of Bcl-2 expression by C/EBP in t(14;18) lymphoma cells

In follicular lymphomas with the t(14;18) translocation, there is increased expression of the bcl-2 gene, which is dependent upon regulatory elements within the bcl-2 5' flanking region and the immunoglobulin heavy-chain gene enhancers. We found that...

Authors: Heckman CA, Wheeler MA and Boxer LM

Disruption of basal JNK activity differentially affects key fibroblast function important for wound healing

We used both a gene knockout approach and pharmacologic modulation to study the implication of the JNK pathway in regulating fibroblast motility, capacity to contract mechanically unloaded collagen gels, and type I collagen gene expression in vitro....

Authors: Javelaud D, Laboureau J, Gabison E, Verrecchia F and Mauviel A

Granulocyte-macrophage colony-stimulating factor and interleukin-3 induce cell cycle progression through the synthesis of c-Myc protein by internal ribosome entry site-mediated translation via phosphatidylinositol 3-kinase pathway in human factor-dependent leukemic cells

Granulocyte-macrophage colony-stimulating factor and interleukin-3 induce cell cycle progression through the synthesis of c-Myc protein by internal ribosome entry site-mediated translation via phosphatidylinositol 3-kinase pathway in human...

Authors: Kobayashi N, Saeki K and Yuo A

Recognition of a novel stage of betaherpesvirus latency in human herpesvirus 6

Latency-associated transcripts of human herpesvirus 6 (H6LTs) (K. Kondo et al. J. Virol. 76:4145-4151, 2002) were maximally expressed at a fairly stable intermediate stage between latency and reactivation both in vivo and in vitro. H6LTs functioned...

Authors: Kondo K, Sashhara J, Shimada K, Takemoto M, Amo K, Miyagawa H and Yamanishi K

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