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The human fascin gene promoter is highly active in mature dendritic cells due to a stage-specific enhancer

Dendritic cells (DC), regarded as the most efficient APCs of the immune system, are capable of activating naive T cells. Thus, DC are primary targets in immunotherapy. However, little is known about gene regulation in DC, and for efficient...

Authors: Bros M, Ross XL, Pautz A, Reske-Kunz AB and Ross R

Chemokine stimulation of human periphereal blood T lymphocytes induces rapid dephosphorylation of ERMs which facilitates loss of microvilli and polarization

Lymphocyte microvilli mediate initial rolling-adhesion along endothelium but are lost during transmigration from circulation to tissue. However, the mechanism for resorption of lymphocyte microvilli remains unexplored. We show that chemokine...

Authors: Brown MJ, Nijhara R, Hallam JA, Gignac M, Yamada KM, Erlandsen SL, Delon J, Kruhlak M and Shaw S

High-resolution multicolor imaging of dynamic signaling complexes in T cells stimulated by planar substrates

The dynamic visualization of developing immunological synapses has been hindered by the difficulty of imaging the contact between the T cell and the antigen-presenting cell (APC). Here, we describe a technique in which T cell responses are...

Authors: Bunnell SC, Barr VA, Fuller CL and Samelson LE

T cell receptor ligation induces the formation of dynamically regulated signaling assemblies.

Tcell antigen receptor (TCR) ligation initiates tyrosine kinase activation, signaling complex assembly, and immune synapse formation. Here, we studied the kinetics and mechanics of signaling complex formation in live Jurkat leukemic T cells using...

Authors: Bunnell SC, Hong DI, Kardon JR, Yamazaki T, McGlade CJ, Barr VA and Samelson LE

Determinants of Notch-3 receptor expression and signaling in vascular smooth muscle cells. Implications in cell-cycle regulation

The Notch family of receptors and ligands plays an important role in cell fate determination, vasculogenesis, and organogenesis. Mutations of the Notch-3 receptor result in an arteriopathy that predisposes to early-onset stroke. However, the...

Authors: Campos AH, Wang W, Pollmann MJ and Gibbons GH

HSP27 inhibits release of mitochondrial protein Smac in multiple myeloma cells and confers dexamethasone resistance

Smac, second mitochondria-derived activator of caspases, promotes apoptosis via activation of caspases. Heat shock protein 27 (Hsp27) negatively regulates another mitochondrial protein, cytochrome c, during apoptosis; however, the role of Hsp27 in...

Authors: Chauhan D, Li G, Hideshima T, Podar K, Mitsiades C, Midsiades N, Catley L, Tai YT, Hayashi T,...

JNK-dependent release of mitochondrial protein, SMAC during apoptosis in multiple myeloma (MM) cells

Smac, second mitochondria-derived activator of caspases, promotes apoptosis via activation of caspases. Previous studies have shown that c-Jun NH(2)-terminal kinase (JNK) is involved in regulating another mitochondrial protein, cytochrome c during...

Authors: Chauhan D, Li G, Hideshima T, Podar K, Mitsiades C, Midsiades N, Munshi N, Kharbanda S and Anderson...

Pericyte-specific expression of RGs5: implications for PDGF and EDG receptor signaling during vascular maturation

RGS proteins finely tune heterotrimeric G-protein signaling. Implying the need for such fine-tuning in the developing vascular system, in situ hybridization revealed a striking and extensive expression pattern of Rgs5 in the arterial walls of...

Authors: Cho H, Kozasa T, Bondjers C, Betsholtz C and Kehrl JH

Pleiotropic defects in lymphocyte activation caused by caspase-8 mutations lead to human immunodeficiency

Apoptosis is a form of programmed cell death that is controlled by aspartate-specific cysteine proteases called caspases. In the immune system, apoptosis counters the proliferation of lymphocytes to achieve a homeostatic balance, which allows potent...

Authors: Chun HJ, Zheng L, Ahmad M, Wang J, Speirs CK, Siegel RM, Dale JK, Puck J, Davis J, Hall CG,...

SST Peer Reviewed

RNA-transfected CD40-activated B cells induce functional T-cell responses against viral and tumor antigen targets: implications for pediatric immunotherapy

Vaccination with antigen-presenting cells (APCs) engineered to mimic mechanisms of immune stimulation represents a promising approach for cancer immunotherapy. Dendritic cell vaccines have entered phase 3 testing in adult malignancies, but such...

Authors: Coughlin CM, Vance BA, Grupp SA and Vonderheide RH

SST Peer Reviewed

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