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4911 results sorted by

Artefactual effects of lipid-based cell transfection reagents on AbetaPP processing and Abeta production

The study of amyloidogenic beta-amyloid precursor protein (AbetaPP) metabolism and amyloid beta protein (Abeta) production has been a major focus of Alzheimer's disease (AD) neuropathogenesis research. Cell transfection is a commonly employed method...
Authors: Romano DM, Dong Y, Hiltunen M, Tanzi RE, Xie Z

Protein-free transfection of CHO host cells with an IgGFusion protein-selection and characterisation of stable high producers and comparison to conventionally transfected clones

In order to improve the current techniques of cell cultivation in the absence of serum, we have developed a protein-free transfection protocol for CHO cells, based on the Nucleofectortrade mark technology. After starting with a heterogeneous pool of...
Authors: Lattenmayer C, Loeschel M, Schriebl K, Sterovsky T, Trummer E, Vorauer-Uhl K, Muller D, Katinger H,...
SST Peer Reviewed

Regulation of leukocyte degranulation by cGMP-dependent protein kinase and phosphoinositide 3-kinase: potential roles in phosphorylation of target membrane SNARE complex proteins in rat mast cells

We examined the roles of cGMP-dependent protein kinase (PKG) and PI3K in degranulation induced by fMLF and by FcepsilonRI cross-linking. In rat basophilic leukemia-2H3 cells expressing formyl peptide receptor, the PKG inhibitors KT5823 and...
Authors: Nanamori M, Chen J, Du X, Ye RD

RNA-based gene therapy for Haemophilia B

Haemophilia B, a deficiency in clotting factor IX (FIX), occurs in about 1 in 25.000 males. Of these patients, approximately 40% are characterized as having 'severe' haemophilia (FIX below 1% of normal). Although the use of plasma-derived or rFIX has...
Authors: Peppelenbosch MP, Milano F, Ferreira CV, Knapisnka A, Diks SH, Spek A

Regulation of apoptosis by a prostate-specific and prostate cancer-associated noncoding gene, PCGEM1

PCGEM1 is a prostate tissue-specific, and prostate cancer-associated noncoding RNA (ncRNA) gene. Previous results revealed a significant association of elevated PCGEM1 expression levels in prostate cancer cells of African-American patients, whose...
Authors: Fu X, Ravindranath L, Tran N, Petrovics G, Srivastava S

Villin Severing Activity Enhances Actin-based Motility In Vivo

Villin, an actin-binding protein associated with the actin bundles that support microvilli, bundles, caps, nucleates, and severs actin in a calcium-dependant manner in vitro. We hypothesized that the severing activity of villin is responsible for its...
Authors: Revenu C, Courtois M, Michelot A, Sykes C, Louvard D, Robine S

Identification of a novel mutation of SH3BP2 in cherubism and demonstration that SH3BP2 mutations lead to increased NFAT activation

We describe a novel missense mutation (Aspartic acid to Asparagine, p.D419N (g.1371G>A, c.1255G>A) within exon 9 of SH3BP2 in a patient with cherubism, an autosomal dominant syndrome characterized by excessive osteoclastic bone resorption of the jaw....
Authors: Lietman SA, Kalinchinko N, Deng X, Kohanski R, Levine MA

Role of GATA-3 in IL-5 gene transcription by CD4+ T cells of asthmatic patients

BACKGROUND: Helper T cells and T cell cytokines play central roles in allergic disorders including bronchial asthma. We reported enhanced IL-5 production by peripheral blood T cells of asthmatic patients. A transcription factor, GATA-3, has been...
Authors: Kaminuma O, Mori A, Kitamura N, Hashimoto T, Kitamura F, Inokuma S, Miyatake S

STIM1 regulates Ca2+ entry via arachidonate-regulated Ca2+-selective (ARC) channels without store-depletion or translocation to the plasma membrane

Recent studies have indicated a critical role for STIM (stromal interacting molecule) proteins in the regulation of the store-operated mode of receptor-activated Ca(2+) entry. Current models emphasize the role of STIM located in the endoplasmic...
Authors: Mignen O, Thompson JL, Shuttleworth TJ

Liver X receptors regulate dendritic cell phenotype and function through blocked induction of the actin-bundling protein fascin

Liver X receptors (LXRs) are nuclear receptors regulating lipid and cholesterol metabolism. Recent data revealed a cross talk between LXR and Toll-like receptor signaling in macrophages, indicating a role in immunity. Here, we show that LXRalpha is...
Authors: Geyeregger R, Zeyda M, Bauer W, Kriehuber E, Saemann MD, Zlabinger GJ, Maurer D, Stulnig TM