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When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay how should I handle the LAL reagent?

Lonza LAL reagent should be handled gently. After reconstitution, the contents of the vial will easily go into solution by either gently inverting or swirling the vial. Vortexing or vigorously shaking the reconstituted LAL reagent will cause it to...

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay how should I prepare my sample for testing?

There are a few ways in which your sample can be prepared before testing it. Most samples only need to be diluted before they are tested with one of our endotoxin detection kits. In order to determine how far out you should dilute your sample, you...

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay what pH should my sample be? What should I adjust it with?

Our endotoxin detection assays are optimized to work with samples with a pH range of 6-8. If the pH of your sample falls outside of this range you can adjust it with a buffer, or HCl or NaOH. Make sure the buffer you are using is endotoxin free by...

When storing my Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor (rFC), endotoxin detection kit the temperature of my refrigerator/freezer has been out of range. Are my reagents ok to use?

The normal storage temperature for LAL products is 2-8°C. The improper storage of the product for a short period of time should not cause a problem with the performance of the kit as long as the components have not been reconstituted. Lyophilized...

What are the advantages of the Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor (rFC), endotoxin detection assays over the rabbit test?

Lonza LAL and PyroGene™ rFC Endotoxin detection assays are faster, have greater sensitivity, show less variability,, are less expensive, and are an alternative to animal model testing. These assays allow higher throughput and can also be automated on...

How should I properly heat my Lonza QCL-1000™ Endpoint LAL Bacterial Endotoxin Test (BET) Assay?

Incubation is another area where assay performance can be affected. We recommend the use of a dry bath and heat block combination that is capable of maintaining 37 ±1 °C. The temperature should be monitored with a calibrated thermometer and checked...

How should I handle the reconstituted Lonza LAL PYROGENT™ Gel Clot Bacterial Endotoxin Testing (BET) Reagent?

Rehydrated LAL reagent may be stored for as long as 24 hours, but it must be kept at 2-8 °C. Do not pre-incubate lysate in the LAL test tubes before adding samples to them. This is not necessary and may cause sensitivity loss in the LAL reagent. If...

How do I handle my Lonza Control Standard Endotoxin (CSE) for Bacterial Endotoxin Testing (BET)?

Lyophilized E. coli endotoxin should be stored at 2-8°C. Endotoxin solutions are generally very stable upon storage except at more dilute concentrations. Rehydrated E. coli endotoxin should be stored refrigerated only (at 2-8°C and not frozen)....

When performing the Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ recombinant Factor C (rFC), endotoxin detection kit how do I know my pipettes are working adequately?

Check your pipetting accuracy. This is important in every phase of LAL testing. Make sure you are adding the correct amounts of material. Good quality graduated pipettes are already calibrated by the manufacturer, but it is up to the user to use them...

When performing the Lonza Bacterial Endotoxin Test (BET), LAL gel clot endotoxin detection kit how do I know if I have a positive result for gel clot?

A positive result is a firm gel which maintains its integrity through inversion to 180°. Resist the temptation to look at any of the test tubes before the hour is up. When reading your test results after the one-hour incubation gently withdraw each...

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