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What percentage of most microvascular lots are lymphatic derived cells (LEC)? What percentage of most microvascular lots are blood vessel derived cells (BEC)?

Each lot of our Human Microvasculare Endothelial Cells - dermal (CC-2543 and CC-2516) and our Human Microvasculare Endothelial Cells - Lung (CC-2527) is tested for the percentage of LEC (double-positive for CD31+ and Podoplanin) and BEC (positive...
Missing: stable linesGuideline

If my cell type of interest is not in the cell list on the 4D Nucleofector® Core Unit, how do I choose the program I want to use?

...type in the program you need.This process will need to be repeated for all cuvettes or strip-wells that require a program not linked to a cell type.To save time with future experiments, you can save this as an experiment or use our Custom Program...
Missing: stable linesGuideline

Do you have any information regarding differentiation of mouse ES cells after Nucleofection® with large plasmids?

It is unlikely that plasmid size would affect in vitro differentiation capability. Gene targeting constructs are often as large as 20 kb and if treated well the ES cells are perfectly capable of generating germ line chimaeras afterwards (indicating...
Missing: stable linesGuideline

After Nucleofection®, how do you determine cell viability?

We determine cell viability after Nucleofection® in two ways: 1) FACS determination of viable/dead cells by PROPIDIUM IODIDE STAINING. We normally analyze transfection efficiency in living cells by FACS: We first exclude cellular debris...
Missing: stable linesGuideline

How many donors are pooled to create the pooled human cells?

Lonza is offering the following primary cells from single donors and from pooled donors:HUVEC (human umbilical vein endothelial cells)NHEK-neo (human epidermal keratinocytes)HMVEC-dNeo (human microvascular endothelial cells, dermal, neonatal)When...
Missing: stable linesGuideline

Do I need a special incubator for cultivation of insect cells ?

Insect cells are cultivated at lower temperatures than E.coli or mammalian cells (S2 cells at 24°C, Sf9 cells at 28°C).Therefore they need a dedicated incubator with appropriate temperature.
Missing: stable linesGuideline

What medium do you recommend for the diseased airway cells?

The recommended medium for diseased cells is the same as for normal cells. BEGM™ for Diseased Bronchial Epithelial Cells, some lots are guaranteed in B-ALI™ Media. SmGM™-2 Medium is recommended for Diseased Bronchial Smooth Muscle Cells. FGM™-2...
Missing: stable linesGuideline

Are there any effects on the differentiation of neural stem cells following Nucleofection®?

The ability of transfected rat or mouse neural stem cells to be subsequently differentiated into a variety of cell types is well documented in:Olig2 Overexpression Induces the In Vitro Differentiation of Neural Stem Cells into Mature...
Missing: stable linesGuideline

How does Lonza test for quality of the diseased airway cells?

Diseased Bronchial Epithelial Cells are characterized by morphology through serial passage and some are tested for differentiation using B-ALI™ Differentiation protocols, these are tested for TEER levels, ciliogenesis, and mucin production. Diseased...
Missing: stable linesGuideline

What age are the rats used to make the rat aortic smooth muscle cells (R-ASM-580)? 

The age of these animals is 6 to 7 weeks.
Missing: stable linesGuideline
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