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What is the general anatomy of the human prostate? From which portion of the human prostate are Lonza's prostate cells are derived?

...the “worker cells” of the tissue or organ.In regards to the prostate specifically, Lonza obtains our Lonza’s Prostate Epithelial Cells (PrEC) from the glandular epithelium (parenchymal portion) of the prostate.The stromal portion of the prostate, or of any...
Missing: stable linesGuideline

What percentage of most microvascular lots are lymphatic derived cells (LEC)? What percentage of most microvascular lots are blood vessel derived cells (BEC)?

Each lot of our Human Microvasculare Endothelial Cells - dermal (CC-2543 and CC-2516) and our Human Microvasculare Endothelial Cells - Lung (CC-2527) is tested for the percentage of LEC (double-positive for CD31+ and Podoplanin) and BEC (positive...
Missing: stable linesGuideline

Do you have any information regarding differentiation of mouse ES cells after Nucleofection® with large plasmids?

It is unlikely that plasmid size would affect in vitro differentiation capability. Gene targeting constructs are often as large as 20 kb and if treated well the ES cells are perfectly capable of generating germ line chimaeras afterwards (indicating...
Missing: stable linesGuideline

What age are the rats used to make the rat aortic smooth muscle cells (R-ASM-580)? 

The age of these animals is 6 to 7 weeks.
Missing: stable linesGuideline

If my cell type of interest is not in the cell list on the 4D Nucleofector® Core Unit, how do I choose the program I want to use?

...type in the program you need.This process will need to be repeated for all cuvettes or strip-wells that require a program not linked to a cell type.To save time with future experiments, you can save this as an experiment or use our Custom Program...
Missing: stable linesGuideline

After Nucleofection®, how do you determine cell viability?

We determine cell viability after Nucleofection® in two ways: 1) FACS determination of viable/dead cells by PROPIDIUM IODIDE STAINING. We normally analyze transfection efficiency in living cells by FACS: We first exclude cellular debris...
Missing: stable linesGuideline

How many donors are pooled to create the pooled human cells?

Lonza is offering the following primary cells from single donors and from pooled donors:HUVEC (human umbilical vein endothelial cells)NHEK-neo (human epidermal keratinocytes)HMVEC-dNeo (human microvascular endothelial cells, dermal, neonatal)When...
Missing: stable linesGuideline

Where are Lonza’s HSMM cells isolated from?

Lonza obtains our Human Skeletal Muscle Myoblasts( HSMM), both diseased and normal, from quadriceps or psoas muscle tissue.
Missing: stable linesGuideline

Do I need a special incubator for cultivation of insect cells ?

Insect cells are cultivated at lower temperatures than E.coli or mammalian cells (S2 cells at 24°C, Sf9 cells at 28°C).Therefore they need a dedicated incubator with appropriate temperature.
Missing: stable linesGuideline

What medium do you recommend for the diseased airway cells?

The recommended medium for diseased cells is the same as for normal cells. BEGM™ for Diseased Bronchial Epithelial Cells, some lots are guaranteed in B-ALI™ Media. SmGM™-2 Medium is recommended for Diseased Bronchial Smooth Muscle Cells. FGM™-2...
Missing: stable linesGuideline
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