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(12)
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(100)
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(67)
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315 results sorted by
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If I use Miltenyi paramagnetic beads for positive selection of
cell
s, i.e. beads remain on the
cell
surface, does that influence Nucleofection® results?
No, it seems that the beads bound to the
cell
surface do not have any influence on Nucleofection®. Larger magnetic beads (i.e. > 1µm) may disturb the Nucleofection® process and negatively influence
cell
viability and efficiency.
Missing:
stable
lines
Are Lonza’s Smooth Muscle
Cell
s contractile or synthetic?
Lonza’s Smooth Muscle
Cells
are synthetic in their proliferating state. As the
cells
begin to differentiate, they become contractile.
Missing:
stable
lines
Which mobilization regime has been used for the CD34+
cell
s from mobilized peripheral blood (4Y-101)? Can I also have the
cell
s from donors treated with another mobilization regime?
The mobilized peripheral blood used for 4Y-101 is derived from peripheral blood mobilized with Neupogen® 10 mcg/kg/day x 5 days. The cryopreserved
cells
can be isolated from day 5 and/or day 6 collections. It is possible to request...
Missing:
stable
lines
What is HEPES? Why is it added to
cell
culture media?
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in
cell
culture, largely because it is better at maintaining physiological pH...
Missing:
stable
lines
Are Lonza's Natural Killer (NK)
cell
s adherent in culture?
Resting Natural Killer
cells
grow in suspension, however, when IL-2 is added to the culture, they will adhere.
Missing:
stable
lines
What donor characteristics are available for the diseased airway
cell
s?
Most Clonetics™ Primary
Cells
have donor information on the Certificate of Analysis (CoA). This information usually includes age, sex, and race. Sometimes additional information may be obtained by contacting Lonza Scientific Support...
Missing:
stable
lines
Approximately what size are the Coronary Artery Smooth Muscle
Cell
s (CASMCs)?
Lonza’s R&D estimates that the CASMC’s are around 30 µm in diameter when rounded/in suspension.
Missing:
stable
lines
Do you have a transfection protocol for
cell
s from clouded leopard or fishing cat?
...of Thailand supports many projects for reproduction and wild life conservation. Now researchers from the Burapho University in Thailand published a protocol for generation of induced pluripotent stem
cells
(iPSCs) from the clouded leopard. With a few somatic...
Missing:
stable
lines
What is the general anatomy of the human prostate? From which portion of the human prostate are Lonza's prostate
cell
s are derived?
...the “worker
cells
” of the tissue or organ.In regards to the prostate specifically, Lonza obtains our Lonza’s Prostate Epithelial
Cells
(PrEC) from the glandular epithelium (parenchymal portion) of the prostate.The stromal portion of the prostate, or of any...
Missing:
stable
lines
What percentage of most microvascular lots are lymphatic derived
cell
s (LEC)? What percentage of most microvascular lots are blood vessel derived
cell
s (BEC)?
Each lot of our Human Microvasculare Endothelial
Cells
- dermal (CC-2543 and CC-2516) and our Human Microvasculare Endothelial
Cells
- Lung (CC-2527) is tested for the percentage of LEC (double-positive for CD31+ and Podoplanin) and BEC (positive...
Missing:
stable
lines
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