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What instrumentation is needed to run the ViaLight™ Plus Assay?  

...are not available the assay can still be performed by adding all reagents manually with a multichannel pipette prior to loading the plate into the luminometer. It is also possible to perform the ViaLight™ Plus Assays in cuvette based luminometers, but this does slow...
Missing: mycoalert

What kind of protocol you recommend for stimulation of human T cells?

For getting the results we present in our datasheet, we recommend the follwing strategy: Cells should be stimulated and cultivated for 3 days before Nucleofection, preferentially on plates coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibody...
Missing: mycoalert

Which cell numbers should I use when running the ToxiLight™ Non-Destructive Cytotoxicity BioAssay

Toxilight works much better at low cell numbers, for adherent cell lines < 10,000 in one well of a 96well plate and < 50,000 for suspension.We would normally seed 10,000 cells/well when using adherent cells to allow enough room in the well...
Missing: mycoalert

Can Lonza’s PrEC cells be co-cultured with Lonza’s PrSC cells?

While Lonza has never attempted to co-culture Prostate Epithelial Cells (PrEC) and Prostate Stromal Cells (PrSC) together in-house, the recommendation of Lonza Research & Development would be to plate and culture the PrEC and PrSC cells together...
Missing: mycoalert

What is the difference between Lonza’s BEGM™ medium and the Growth Media portion of Lonza’s B-ALI BulletKit™ Medium?

...for thawing, plating, and expanding the cells through one passage prior to differentiation (the maximum amount of expansion that is guaranteed prior to differentiation), hence, if using Lonza’s ALI screened cells and ALI differentiation media, there should...
Missing: mycoalert

What is the difference between Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium?

...for thawing, plating, and expanding the cells through one passage prior to differentiation (the maximum amount of expansion that is guaranteed prior to differentiation), hence, if using Lonza’s ALI screened cells and ALI differentiation media, there should...
Missing: mycoalert

How do I prevent keratinocyte terminal differentiation towards corneocytes? Can Lonza’s keratinocytes be cultured without calcium?

...offers most of our keratinocyte media in calcium free varieties, it is still required to add calcium. Without calcium, keratinocytes will not attach or proliferate. To retain good plating and survival of keratinocytes, Lonza suggests adding calcium...
Missing: mycoalert

How do I handle my Lonza Control Standard Endotoxin (CSE) for Bacterial Endotoxin Testing (BET)?

...they are kept because endotoxin can “plate out” on irregular or dirty glass surfaces. Since some types of plastic have been shown to have an affinity for endotoxin, the use of plastic containers to prepare and store endotoxin must be approached with caution...
Missing: mycoalert

PyroTec Pro: Is it possible to reload the instrument during a run with reagents, consumables, samples? Can you continuously load? Is an automatic retest supported?

After the last microplate is placed in the reader and control is transferred to the WinKQCL™ Software to begin reading the plate, the instrument’s work is complete. At that time, it would be possible to start preparing the deck for the next run...
Missing: mycoalert

Why am I not able to detect my fluorescent labeled siRNA (e.g. FITC) after 24 or 48 hours post-Nucleofection?

...analysis. Some labels are also subject to photo bleaching, pH changes, and may exhibit cell type specific effects as well. To minimize FITC-bleaching it may help to wrap the culture plates in aluminum foil immediately after Nucleofection™. So...
Missing: mycoalert
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