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Which mobilization regime has been used for the CD34+ cells from mobilized peripheral blood (4Y-101)? Can I also have the cells from donors treated with another mobilization regime?

The mobilized peripheral blood used for 4Y-101 is derived  from peripheral blood mobilized with Neupogen® 10 mcg/kg/day x 5 days. The cryopreserved cells can be isolated from day 5 and/or day 6 collections. It is possible to request...
Missing: stable linesGuideline

What are the suggested culturing conditions for CD34+ cells?

Lonza's CD34+ cells should be cultured according to Lonza's "Technical information and Instructions for Culturing Human CD34+ Cells"Upon thaw, we typically suggest that CD34+ cells be established in culture at a density of 20,000 to 200,000...
Missing: stable linesGuideline

If I use Miltenyi paramagnetic beads for positive selection of cells, i.e. beads remain on the cell surface, does that influence Nucleofection® results?

No, it seems that the beads bound to the cell surface do not have any influence on Nucleofection®. Larger magnetic beads (i.e. > 1µm) may disturb the Nucleofection® process and negatively influence cell viability and efficiency.
Missing: stable linesGuideline

Where are Lonza’s dermal cells isolated from?

Lonza is offering different dermal cell types, which are either isolated from adult or from neonatal tissue.Lonza’s adult dermal cells (including keratinocytes, microvascular endothelial cells, fibroblasts, and melanocytes) are usually isolated from...
Missing: stable linesGuideline

Are Lonza’s Smooth Muscle Cells contractile or synthetic?

Lonza’s Smooth Muscle Cells are synthetic in their proliferating state. As the cells begin to differentiate, they become contractile.
Missing: stable linesGuideline

Approximately what size are the Coronary Artery Smooth Muscle Cells (CASMCs)?

Lonza’s R&D estimates that the CASMC’s are around 30 µm in diameter when rounded/in suspension.
Missing: stable linesGuideline

What is HEPES? Why is it added to cell culture media?

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH...
Missing: stable linesGuideline

Are Lonza's Natural Killer (NK) cells adherent in culture?

Resting Natural Killer cells grow in suspension, however, when IL-2 is added to the culture, they will adhere.
Missing: stable linesGuideline

What donor characteristics are available for the diseased airway cells?

Most Clonetics™ Primary Cells have donor information on the Certificate of Analysis (CoA). This information usually includes age, sex, and race. Sometimes additional information may be obtained by contacting Lonza Scientific Support...
Missing: stable linesGuideline

Do you have a transfection protocol for cells from clouded leopard or fishing cat?

...of Thailand supports many projects for reproduction and wild life conservation. Now researchers from the Burapho University in Thailand published a protocol for generation of induced pluripotent stem cells (iPSCs) from the clouded leopard. With a few somatic...
Missing: stable linesGuideline
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