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Do Lonza's NK cells proliferate in culture?

Publications indicate that IL-2 and IL-21 can stimulate NK cell proliferation in addition to using a human serum supplemented medium.
Missing: stable lines

I want to stimulate Human T cells post Nucleofection®. Are there any precautions to keep in mind?

After Nucleofection®, wait at least four hours before stimulation. Stimulating immediately after Nucleofection® may lead to increased cell mortality and poor activation of cells. For more details on the stimulation of Human T cells post...
Missing: stable lines

How do I cryopreserve Lonza’s primary cells? What media cocktail are Lonza’s primary cells cryopreserved in?

Please be aware that cryopreservation may compromise cell quality and performance. Lonza CANNOT guarantee performance of Clonetics™ & Poietics™ Cells that have been cryopreserved outside of Lonza. To avoid loss of cells and forfeiture of your...
Missing: stable lines

Why do you have different Optimized Protocols for the Nucleofection® of unstimulated and stimulated Human T cells?

We found that the stimulation of T cells not only changes the cells' function but also significantly alters the requirements for successful Nucleofection®. That's why Lonza developed two Optimized Protocols for transfection giving different...
Missing: stable lines

Where are Lonza’s lung microvascular endothelial cells (HMVEC-L) isolated from?

Lonza’s lung microvascular endothelial cells (HMVEC-L) are isolated from the tissue directly beneath the outer lining of the lung. All vessels isolated are smaller than can be seen with the naked eye.
Missing: stable

Where are Lonza’s HRE Human Renal Epithelial cells CC-2556 isolated from?

Lonza’s Human Renal Epithelial (HRE) cells are an expansion of HNK (human neonatal kidney) cells. The kidneys are homogenized and the mixed population of cells are cultured in Lonza REGM™ Renal Epithelial Cell Growth Medium for one passage prior...
Missing: stable lines

Why is the morphology of Lonza’s lung microvascular endothelial cells (HMVEC-L) CC-2527 different from most other endothelial cells?

While most endothelial cells will have a “paving stone” type morphology, Lonza’s lung microvascular endothelial cells (HMVEC-L) will generally appear more stretched and elongated, especially at low confluence. This is a normal morphological...
Missing: stable lines

What are the suggested culturing conditions for CD34+ cells?

Lonza's CD34+ cells should be cultured according to Lonza's "Technical information and Instructions for Culturing Human CD34+ Cells"Upon thaw, we typically suggest that CD34+ cells be established in culture at a density of 20,000 to 200,000...
Missing: stable lines

Where are Lonza’s dermal cells isolated from?

Lonza is offering different dermal cell types, which are either isolated from adult or from neonatal tissue.Lonza’s adult dermal cells (including keratinocytes, microvascular endothelial cells, fibroblasts, and melanocytes) are usually isolated from...
Missing: stable lines

If I use Miltenyi paramagnetic beads for positive selection of cells, i.e. beads remain on the cell surface, does that influence Nucleofection® results?

No, it seems that the beads bound to the cell surface do not have any influence on Nucleofection®. Larger magnetic beads (i.e. > 1µm) may disturb the Nucleofection® process and negatively influence cell viability and efficiency.
Missing: stable lines
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