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307 results sorted by
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Do Lonza's NK
cell
s proliferate in culture?
Publications indicate that IL-2 and IL-21 can stimulate NK
cell
proliferation in addition to using a human serum supplemented medium.
Missing:
stable
lines
Where are Lonza’s lung microvascular endothelial
cell
s (HMVEC-L) isolated from?
Lonza’s lung microvascular endothelial
cells
(HMVEC-L) are isolated from the tissue directly beneath the outer
lining
of the lung. All vessels isolated are smaller than can be seen with the naked eye.
Missing:
stable
I want to stimulate Human T
cell
s post Nucleofection®. Are there any precautions to keep in mind?
After Nucleofection®, wait at least four hours before stimulation. Stimulating immediately after Nucleofection® may lead to increased
cell
mortality and poor activation of
cells
. For more details on the stimulation of Human T
cells
post...
Missing:
stable
lines
How do I cryopreserve Lonza’s primary
cell
s? What media cocktail are Lonza’s primary
cell
s cryopreserved in?
Please be aware that cryopreservation may compromise
cell
quality and performance. Lonza CANNOT guarantee performance of Clonetics™ & Poietics™
Cells
that have been cryopreserved outside of Lonza. To avoid loss of
cells
and forfeiture of your...
Missing:
stable
lines
Why do you have different Optimized Protocols for the Nucleofection® of unstimulated and stimulated Human T
cell
s?
We found that the stimulation of T
cells
not only changes the
cells
' function but also significantly alters the requirements for successful Nucleofection®. That's why Lonza developed two Optimized Protocols for transfection giving different...
Missing:
stable
lines
Where are Lonza’s HRE Human Renal Epithelial
cell
s CC-2556 isolated from?
Lonza’s Human Renal Epithelial (HRE)
cells
are an expansion of HNK (human neonatal kidney)
cells
. The kidneys are homogenized and the mixed population of
cells
are cultured in Lonza REGM™ Renal Epithelial
Cell
Growth Medium for one passage prior...
Missing:
stable
lines
Why is the morphology of Lonza’s lung microvascular endothelial
cell
s (HMVEC-L) CC-2527 different from most other endothelial
cell
s?
While most endothelial
cells
will have a “paving stone” type morphology, Lonza’s lung microvascular endothelial
cells
(HMVEC-L) will generally appear more stretched and elongated, especially at low confluence. This is a normal morphological...
Missing:
stable
lines
Which mobilization regime has been used for the CD34+
cell
s from mobilized peripheral blood (4Y-101)? Can I also have the
cell
s from donors treated with another mobilization regime?
The mobilized peripheral blood used for 4Y-101 is derived from peripheral blood mobilized with Neupogen® 10 mcg/kg/day x 5 days. The cryopreserved
cells
can be isolated from day 5 and/or day 6 collections. It is possible to request...
Missing:
stable
lines
If I use Miltenyi paramagnetic beads for positive selection of
cell
s, i.e. beads remain on the
cell
surface, does that influence Nucleofection® results?
No, it seems that the beads bound to the
cell
surface do not have any influence on Nucleofection®. Larger magnetic beads (i.e. > 1µm) may disturb the Nucleofection® process and negatively influence
cell
viability and efficiency.
Missing:
stable
lines
What are the suggested culturing conditions for CD34+
cell
s?
Lonza's CD34+
cells
should be cultured according to Lonza's "Technical information and Instructions for Culturing Human CD34+
Cells
"Upon thaw, we typically suggest that CD34+
cells
be established in culture at a density of 20,000 to 200,000...
Missing:
stable
lines
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