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(320)
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(16)
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(14)
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(14)
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(14)
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(7)
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(6)
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(101)
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(68)
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(39)
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(26)
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320 results sorted by
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How can
cell
s be cured of mycoplasma contamination?
Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™ Mycoplasma Elimination Reagent has been optimized to eliminate mycoplasma with minimal toxic effects on the
cells
. The MycoZap™ Reagent eliminates...
Missing:
stable
lines
How can
cell
s be cured of mycoplasma contamination?
Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™ Mycoplasma Elimination Reagent has been optimized to eliminate mycoplasma with minimal toxic effects on the
cells
.The MycoZap™ Reagent eliminates...
Missing:
stable
lines
Do Lonza's NK
cell
s proliferate in culture?
Publications indicate that IL-2 and IL-21 can stimulate NK
cell
proliferation in addition to using a human serum supplemented medium.
Missing:
stable
lines
What are useful methods to enrich/purify specific hematopoetic
cell
populations before Nucleofection™?
Specific blood
cell
populations can be enriched/purified for Nucleofection™ by MACS™ selection Kits (Miltenyi Biotec GmbH), RosetteSep™ Kits (Stem
Cell
Technologies Inc.) or Dynal™ beads (Invitrogen Corporation).
Missing:
stable
lines
How do I cryopreserve Lonza’s primary
cell
s? What media cocktail are Lonza’s primary
cell
s cryopreserved in?
Please be aware that cryopreservation may compromise
cell
quality and performance. Lonza CANNOT guarantee performance of Clonetics™ & Poietics™
Cells
that have been cryopreserved outside of Lonza. To avoid loss of
cells
and forfeiture of your...
Missing:
stable
lines
I want to stimulate Human T
cell
s post Nucleofection®. Are there any precautions to keep in mind?
After Nucleofection®, wait at least four hours before stimulation. Stimulating immediately after Nucleofection® may lead to increased
cell
mortality and poor activation of
cells
. For more details on the stimulation of Human T
cells
post...
Missing:
stable
lines
Why do you have different Optimized Protocols for the Nucleofection® of unstimulated and stimulated Human T
cell
s?
We found that the stimulation of T
cells
not only changes the
cells
' function but also significantly alters the requirements for successful Nucleofection®. That's why Lonza developed two Optimized Protocols for transfection giving different...
Missing:
stable
lines
Where are Lonza’s HRE Human Renal Epithelial
cell
s CC-2556 isolated from?
Lonza’s Human Renal Epithelial (HRE)
cells
are an expansion of HNK (human neonatal kidney)
cells
. The kidneys are homogenized and the mixed population of
cells
are cultured in Lonza REGM™ Renal Epithelial
Cell
Growth Medium for one passage prior...
Missing:
stable
lines
Why is the morphology of Lonza’s lung microvascular endothelial
cell
s (HMVEC-L) CC-2527 different from most other endothelial
cell
s?
While most endothelial
cells
will have a “paving stone” type morphology, Lonza’s lung microvascular endothelial
cells
(HMVEC-L) will generally appear more stretched and elongated, especially at low confluence. This is a normal morphological...
Missing:
stable
lines
If I use Miltenyi paramagnetic beads for positive selection of
cell
s, i.e. beads remain on the
cell
surface, does that influence Nucleofection® results?
No, it seems that the beads bound to the
cell
surface do not have any influence on Nucleofection®. Larger magnetic beads (i.e. > 1µm) may disturb the Nucleofection® process and negatively influence
cell
viability and efficiency.
Missing:
stable
lines
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