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What are the size of CD34+ Cells?

When resting, they are about 8-10 microns.
Missing: stable linesGuideline

Do Lonza's NK cells proliferate in culture?

Publications indicate that IL-2 and IL-21 can stimulate NK cell proliferation in addition to using a human serum supplemented medium.
Missing: stable linesGuideline

I want to stimulate Human T cells post Nucleofection®. Are there any precautions to keep in mind?

After Nucleofection®, wait at least four hours before stimulation. Stimulating immediately after Nucleofection® may lead to increased cell mortality and poor activation of cells. For more details on the stimulation of Human T cells post...
Missing: stable linesGuideline

How do I cryopreserve Lonza’s primary cells? What media cocktail are Lonza’s primary cells cryopreserved in?

Please be aware that cryopreservation may compromise cell quality and performance. Lonza CANNOT guarantee performance of Clonetics™ & Poietics™ Cells that have been cryopreserved outside of Lonza. To avoid loss of cells and forfeiture of your...
Missing: stable linesGuideline

Why do you have different Optimized Protocols for the Nucleofection® of unstimulated and stimulated Human T cells?

We found that the stimulation of T cells not only changes the cells' function but also significantly alters the requirements for successful Nucleofection®. That's why Lonza developed two Optimized Protocols for transfection giving different...
Missing: stable linesGuideline

What should I pay attention to when transferring my reporter gene set up from a cell line to a primary cell?

In general the overall expression level differs between different cell types. Don't expect the same level of gene induction when working with primary cells. You might need to change to other DNA amounts or ratios.
Missing: stable linesGuideline

Where are Lonza’s HRE Human Renal Epithelial cells CC-2556 isolated from?

Lonza’s Human Renal Epithelial (HRE) cells are an expansion of HNK (human neonatal kidney) cells. The kidneys are homogenized and the mixed population of cells are cultured in Lonza REGM™ Renal Epithelial Cell Growth Medium for one passage prior...
Missing: stable linesGuideline

When nucleofecting cancer cell lines, do I need to be concerned about passage number?

For the most efficient gene transfer, we recommend using cells that are in logarithmic growth phase and at a passage number of 10-15 (from the time of thaw). This is because some cell lines differentiate and change their features after many passages.
Missing: stable linesGuideline

Why is the morphology of Lonza’s lung microvascular endothelial cells (HMVEC-L) CC-2527 different from most other endothelial cells?

While most endothelial cells will have a “paving stone” type morphology, Lonza’s lung microvascular endothelial cells (HMVEC-L) will generally appear more stretched and elongated, especially at low confluence. This is a normal morphological...
Missing: stable linesGuideline

Which mobilization regime has been used for the CD34+ cells from mobilized peripheral blood (4Y-101)? Can I also have the cells from donors treated with another mobilization regime?

The mobilized peripheral blood used for 4Y-101 is derived  from peripheral blood mobilized with Neupogen® 10 mcg/kg/day x 5 days. The cryopreserved cells can be isolated from day 5 and/or day 6 collections. It is possible to request...
Missing: stable linesGuideline
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