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I thawed and plated my Clonetics™ embryonic rat or mouse neuronal
cell
s and there are a lot of dead
cell
s. What happened?
Cell
death will usually be observed during the first few days of growth, resulting in
cell
debris in the culture – this is normal.
Cell
cultivation should be continued and surviving
cells
should start to develop. By day 4, neurite networks...
Missing:
stable
lines
What is the difference between the different
cell
sources (bone marrow, cord blood, or fetal liver) for CD34+
cell
s?
Bone Marrow: provides a hematopoietic microenvironment and the highest number of different donors.Cord Blood: more naive
cells
useful in transplant and stem
cell
research.Fetal Liver: best for developmental studies and they are highly proliferative...
Missing:
stable
lines
Pyro
Cell
® MAT System Kit: Can I use a different
cell
culture supplement during the stimulation of pMAT
cell
s?
Alternative supplements may impact the reactivity of the pMAT
cells
towards endotoxin and Non-endotoxin Pyrogens (NEPs). If an alternative supplement is used, a new qualification of the MAT test is required. The optimized MAT
cell
culture...
Missing:
stable
lines
What are the size of CD34+
Cell
s?
When resting, they are about 8-10 microns.
Missing:
stable
lines
How are Lonza's CD34+
cell
s isolated?
Lonza's CD34+
cells
are isolated first by obtaining mononuclear
cells
from bone marrow or cord blood via density gradient centrifugation. The CD34+
cells
are then isolated form the mononuclear
cell
population using positive immunomagnetic selection.
Missing:
stable
lines
What is the purity of Lonza's CD34+
cell
s?
Cell
purity (as determined via FACS analysis for CD34 expression) is guaranteed to be 90% or greater for all Lonza CD34+
cell
products. For some Lonza CD34+
cell
products, a "budget" version of the product is available. The "budget" versions...
Missing:
stable
lines
What are useful methods to enrich/purify specific hematopoetic
cell
populations before Nucleofection™?
Specific blood
cell
populations can be enriched/purified for Nucleofection™ by MACS™ selection Kits (Miltenyi Biotec GmbH), RosetteSep™ Kits (Stem
Cell
Technologies Inc.) or Dynal™ beads (Invitrogen Corporation).
Missing:
stable
lines
How to maximize
cell
viability in a Nucleofection® experiment?
Pay careful attention to centrifugation speeds prior to Nucleofection®; be sure not to centrifuge the
cells
at higher than 90xg. Any trypsinization should use the minimal amount of reagent and time necessary in order to minimize stress to the
cells
...
Missing:
stable
lines
How can
cell
s be cured of mycoplasma contamination?
Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™ Mycoplasma Elimination Reagent has been optimized to eliminate mycoplasma with minimal toxic effects on the
cells
. The MycoZap™ Reagent eliminates...
Missing:
stable
lines
How can
cell
s be cured of mycoplasma contamination?
Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™ Mycoplasma Elimination Reagent has been optimized to eliminate mycoplasma with minimal toxic effects on the
cells
.The MycoZap™ Reagent eliminates...
Missing:
stable
lines
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