Data Type
Cell Information
(514)
Citations
(4760)
FAQ
(307)
Culture Media
(45)
Category
Primary Cells and Media
(161)
Transfection
(106)
Bioassay
(31)
Mycoplasma Detection and Prevention
(16)
Laboratory Instrumentation
(14)
Serum-free and Speciality Media
(11)
Cell Lines and Primary Cancer Cells
(7)
3D Cell Culture
(5)
Classical Media and Reagents
(5)
Endotoxin and Pyrogen Testing
(4)
Cell Services
(3)
Bioprocess Containers
(2)
Live Cell Imaging
(1)
+ Show All
Research Area
Basic Research
(100)
Cancer Research/Cell Biology
(67)
Immunotherapy / Hematology
(54)
Gene Expression
(39)
Stem Cells
(34)
Respiratory Research
(26)
Cardiovascular
(21)
Neurobiology
(18)
Regenerative medicine
(18)
Uncategorized
(18)
Toxicology
(16)
Dermatology/Tissue Engineering
(8)
Drug Discovery
(6)
Molecular Biology
(5)
Endotoxin Testing
(3)
Gastroenterology
(1)
Parasitology
(1)
+ Show All
307 results sorted by
relevance
alphabetical
newest first
oldest first
How are Lonza's mononuclear
cell
s isolated?
Lonza's mononuclear
cells
are isolated from bone marrow, cord blood, or peripheral blood via density gradient centrifugation
Missing:
stable
lines
I thawed and plated my Clonetics™ embryonic rat or mouse neuronal
cell
s and there are a lot of dead
cell
s. What happened?
Cell
death will usually be observed during the first few days of growth, resulting in
cell
debris in the culture – this is normal.
Cell
cultivation should be continued and surviving
cells
should start to develop. By day 4, neurite networks...
Missing:
stable
lines
What is the difference between the different
cell
sources (bone marrow, cord blood, or fetal liver) for CD34+
cell
s?
Bone Marrow: provides a hematopoietic microenvironment and the highest number of different donors.Cord Blood: more naive
cells
useful in transplant and stem
cell
research.Fetal Liver: best for developmental studies and they are highly proliferative...
Missing:
stable
lines
What are useful methods to enrich/purify specific hematopoetic
cell
populations before Nucleofection™?
Specific blood
cell
populations can be enriched/purified for Nucleofection™ by MACS™ selection Kits (Miltenyi Biotec GmbH), RosetteSep™ Kits (Stem
Cell
Technologies Inc.) or Dynal™ beads (Invitrogen Corporation).
Missing:
stable
lines
I want to stimulate Human T
cell
s post Nucleofection®. Are there any precautions to keep in mind?
After Nucleofection®, wait at least four hours before stimulation. Stimulating immediately after Nucleofection® may lead to increased
cell
mortality and poor activation of
cells
. For more details on the stimulation of Human T
cells
post...
Missing:
stable
lines
Where are Lonza’s lung microvascular endothelial
cell
s (HMVEC-L) isolated from?
Lonza’s lung microvascular endothelial
cells
(HMVEC-L) are isolated from the tissue directly beneath the outer
lining
of the lung. All vessels isolated are smaller than can be seen with the naked eye.
Missing:
stable
Why do you have different Optimized Protocols for the Nucleofection® of unstimulated and stimulated Human T
cell
s?
We found that the stimulation of T
cells
not only changes the
cells
' function but also significantly alters the requirements for successful Nucleofection®. That's why Lonza developed two Optimized Protocols for transfection giving different...
Missing:
stable
lines
How are Lonza's CD34+
cell
s isolated?
Lonza's CD34+
cells
are isolated first by obtaining mononuclear
cells
from bone marrow or cord blood via density gradient centrifugation. The CD34+
cells
are then isolated form the mononuclear
cell
population using positive immunomagnetic selection.
Missing:
stable
lines
What is the purity of Lonza's CD34+
cell
s?
Cell
purity (as determined via FACS analysis for CD34 expression) is guaranteed to be 90% or greater for all Lonza CD34+
cell
products. For some Lonza CD34+
cell
products, a "budget" version of the product is available. The "budget" versions...
Missing:
stable
lines
How to maximize
cell
viability in a Nucleofection® experiment?
Pay careful attention to centrifugation speeds prior to Nucleofection®; be sure not to centrifuge the
cells
at higher than 90xg. Any trypsinization should use the minimal amount of reagent and time necessary in order to minimize stress to the
cells
...
Missing:
stable
lines
PREVIOUS
PAGE
3
PAGE
4
PAGE
5
PAGE
6
PAGE
7
PAGE
8
PAGE
9
PAGE
10
PAGE
11
NEXT