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I thawed and plated my Clonetics™ embryonic rat or mouse neuronal cells and there are a lot of dead cells. What happened?

Cell death will usually be observed during the first few days of growth, resulting in cell debris in the culture – this is normal. Cell cultivation should be continued and surviving cells should start to develop. By day 4, neurite networks...
Missing: stable lines

What is the difference between the different cell sources (bone marrow, cord blood, or fetal liver) for CD34+ cells?

Bone Marrow: provides a hematopoietic microenvironment and the highest number of different donors.Cord Blood: more naive cells useful in transplant and stem cell research.Fetal Liver: best for developmental studies and they are highly proliferative...
Missing: stable lines

What are useful methods to enrich/purify specific hematopoetic cell populations before Nucleofection™?

Specific blood cell populations can be enriched/purified for Nucleofection™ by MACS™ selection Kits (Miltenyi Biotec GmbH), RosetteSep™ Kits (StemCell Technologies Inc.) or Dynal™ beads (Invitrogen Corporation).
Missing: stable lines

Why do I need to store cell culture media in the dark?

...photosensitive than other cell types.Cell line sensitivity: Light-induced media cytotoxicity is a general phenomenon, but different cell lines have different tolerances to light-exposed media. For example, the human B-cell, RPMI 1788, required a sixfold higher...
Missing: stable

I want to stimulate Human T cells post Nucleofection®. Are there any precautions to keep in mind?

After Nucleofection®, wait at least four hours before stimulation. Stimulating immediately after Nucleofection® may lead to increased cell mortality and poor activation of cells. For more details on the stimulation of Human T cells post...
Missing: stable lines

Why do you have different Optimized Protocols for the Nucleofection® of unstimulated and stimulated Human T cells?

We found that the stimulation of T cells not only changes the cells' function but also significantly alters the requirements for successful Nucleofection®. That's why Lonza developed two Optimized Protocols for transfection giving different...
Missing: stable lines

Where are Lonza’s lung microvascular endothelial cells (HMVEC-L) isolated from?

Lonza’s lung microvascular endothelial cells (HMVEC-L) are isolated from the tissue directly beneath the outer lining of the lung. All vessels isolated are smaller than can be seen with the naked eye.
Missing: stable

How are Lonza's CD34+ cells isolated?

Lonza's CD34+ cells are isolated first by obtaining mononuclear cells from bone marrow or cord blood via density gradient centrifugation. The CD34+ cells are then isolated form the mononuclear cell population using positive immunomagnetic selection.
Missing: stable lines

What is the purity of Lonza's CD34+ cells?

Cell purity (as determined via FACS analysis for CD34 expression) is guaranteed to be 90% or greater for all Lonza CD34+ cell products. For some Lonza CD34+ cell products, a "budget" version of the product is available. The "budget" versions...
Missing: stable lines

How to maximize cell viability in a Nucleofection® experiment?

Pay careful attention to centrifugation speeds prior to Nucleofection®; be sure not to centrifuge the cells at higher than 90xg. Any trypsinization should use the minimal amount of reagent and time necessary in order to minimize stress to the cells...
Missing: stable lines
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