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What is the difference between the different cell sources (bone marrow, cord blood, or fetal liver) for CD34+ cells?

Bone Marrow: provides a hematopoietic microenvironment and the highest number of different donors.Cord Blood: more naive cells useful in transplant and stem cell research.Fetal Liver: best for developmental studies and they are highly proliferative...
Missing: stable lines

PyroCell® MAT System Kit: Can I use a different cell culture supplement during the stimulation of pMAT cells?

Alternative supplements may impact the reactivity of the pMAT cells towards endotoxin and Non-endotoxin Pyrogens (NEPs). If an alternative supplement is used, a new qualification of the MAT test is required. The optimized MAT cell culture...
Missing: stable lines

Is it possible to use frozen primary CD34+ hematopoietic stem cells for Nucleofection®?

Yes. For cryopreserved PBMCs or enriched CD34 populations, we recommend culturing the thawed cells 1-2 hours in culture medium before Nucleofection®. Any further enrichment procedure after thawing is not recommended. Viability of frozen nucleofected...
Missing: stable lines

Can calcium influx issues affect viability or differentiation in Stem Cells after Nucleofection®?

When cells are transfected by Nucleofection®, transient pores are generated in the cell membrane. Generally, these pores disappear within 15 minutes of transfection, but if cells are plated immediately after Nucleofection® in medias...
Missing: stable lines

Why do I need to store cell culture media in the dark?

...photosensitive than other cell types.Cell line sensitivity: Light-induced media cytotoxicity is a general phenomenon, but different cell lines have different tolerances to light-exposed media. For example, the human B-cell, RPMI 1788, required a sixfold higher...
Missing: stable

Where are Lonza’s lung microvascular endothelial cells (HMVEC-L) isolated from?

Lonza’s lung microvascular endothelial cells (HMVEC-L) are isolated from the tissue directly beneath the outer lining of the lung. All vessels isolated are smaller than can be seen with the naked eye.
Missing: stable

What are the size of CD34+ Cells?

When resting, they are about 8-10 microns.
Missing: stable lines

How are Lonza's CD34+ cells isolated?

Lonza's CD34+ cells are isolated first by obtaining mononuclear cells from bone marrow or cord blood via density gradient centrifugation. The CD34+ cells are then isolated form the mononuclear cell population using positive immunomagnetic selection.
Missing: stable lines

What is the purity of Lonza's CD34+ cells?

Cell purity (as determined via FACS analysis for CD34 expression) is guaranteed to be 90% or greater for all Lonza CD34+ cell products. For some Lonza CD34+ cell products, a "budget" version of the product is available. The "budget" versions...
Missing: stable lines

How to maximize cell viability in a Nucleofection® experiment?

Pay careful attention to centrifugation speeds prior to Nucleofection®; be sure not to centrifuge the cells at higher than 90xg. Any trypsinization should use the minimal amount of reagent and time necessary in order to minimize stress to the cells...
Missing: stable lines
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