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How can I improve Human Hepatocyte viability post Nucleofection™ on the 96-well Shuttle™ device?

...in the Nucleocuvette™ plate after Nucleofection. This can be done by adding to each well, 180ul Ficoll solution (25% Ficoll in Plating medium Lonza Basal Medium (Cat.No.:CC-3198) supplemented with BulletKit) and centrifuging the cells in the Nucleocuvette for 4...
Missing: mycoalert

When can I start induction studies on Human Hepatocytes transfected with the Nucleofector®?

We recommend allowing the transfected hepatocytes to recover in Plating medium for 72 hours post-Nucleofection®.For induction studies (e.g. CYP3A4), the Plating medium is replaced with maintenance medium (William's E without FCS and EGF...
Missing: mycoalert

What cell number do I need per well when using the 96-well Shuttle® Device or the 4D-Nucleocuvette® Stripes?

...cell number per well ranges from 104 and 106 cells. The lower limits are often dictated by the minimum amount of cells required for optimal plating density on 96-well cell culture plates so that one may expect that even lower cell numbers might work...
Missing: mycoalert

How should I properly heat my Lonza QCL-1000™ Endpoint LAL Bacterial Endotoxin Test (BET) Assay?

...and checked before each use of the dry bath. The rapid rate of the LAL reaction requires quick and uniform heat transfer. A warm air incubator is not as efficient at heat transfer as a dry bath. In a microtiter plate, edge effects can cause the different...
Missing: mycoalert

PyroTec Pro: How long does the assay take, and can an assay be run overnight?

Typical runtime from start of the automated microplate setup to completion of WinKQCL™ Software reading the plate for a single assay is 1.5 hours – 2.5 hours, depending on the number of samples and the complexity of the dilutions. Therefore...
Missing: mycoalert

What equipment do I need to run my Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay?

...Kinetic-QCL™ or PYROGENT™-5000, an incubating kinetic plate reader and software is required. This plate reader should have a shaking function, be able to incubate at 37°C for the duration of the assay, and take kinetic readings at 405 nm (for Kinetic-QCL...
Missing: mycoalert

I want to stimulate human primary T-cells with anti-CD3 and anti-CD28. Do you have a detailed protocol for this?

Yes, the detailed protocol is part of our 4D-Nucleofector® Protocol for stimulated Human T Cells.For optimal stimulation of the transfected cells we recommend coating 96-wells plates for stimulation [Nunc Immuno™ Plate C96 Maxi Sorp™, Cat. No. 430...
Missing: mycoalert

What media should be used for spheroid formation with hepatocytes?

Lonza's QC uses HCM (CC-3198) supplemented with 20% FBS and 25 mM Hepes. No plating media is necessary when seeding for spheroids. 
Missing: mycoalert

How many culture/trans-wells does your B-ALI™ Media Kit kit provide?

1 Ampoule of Normal Human Bronchial Epithelial Cells (NHBE) + 1 B-ALI™ Media Kit will make one 24-well plate for at least 1 month of culture & cells will display beating cilia within 25 days.
Missing: mycoalert

How do I promote keratinocyte terminal differentiation towards corneocytes?

Keratinocyte differentiation can be induced by increasing the concentration of calcium and/or by adding serum to the media. Cells should be initially plated using the standard culture media and conditions and allowed ample time to recover from...
Missing: mycoalert
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