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When can I start induction studies on Human Hepatocytes transfected with the Nucleofector®?

We recommend allowing the transfected hepatocytes to recover in Plating medium for 72 hours post-Nucleofection®.For induction studies (e.g. CYP3A4), the Plating medium is replaced with maintenance medium (William's E without FCS and EGF...
Missing: mycoalert

What cell number do I need per well when using the 96-well Shuttle® Device or the 4D-Nucleocuvette® Stripes?

...cell number per well ranges from 104 and 106 cells. The lower limits are often dictated by the minimum amount of cells required for optimal plating density on 96-well cell culture plates so that one may expect that even lower cell numbers might work...
Missing: mycoalert

How should I properly heat my Lonza QCL-1000™ Endpoint LAL Bacterial Endotoxin Test (BET) Assay?

...and checked before each use of the dry bath. The rapid rate of the LAL reaction requires quick and uniform heat transfer. A warm air incubator is not as efficient at heat transfer as a dry bath. In a microtiter plate, edge effects can cause the different...
Missing: mycoalert

PyroTec Pro: How long does the assay take, and can an assay be run overnight?

Typical runtime from start of the automated microplate setup to completion of WinKQCL™ Software reading the plate for a single assay is 1.5 hours – 2.5 hours, depending on the number of samples and the complexity of the dilutions. Therefore...
Missing: mycoalert

What equipment do I need to run my Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay?

...Kinetic-QCL™ or PYROGENT™-5000, an incubating kinetic plate reader and software is required. This plate reader should have a shaking function, be able to incubate at 37°C for the duration of the assay, and take kinetic readings at 405 nm (for Kinetic-QCL...
Missing: mycoalert

I want to stimulate human primary T-cells with anti-CD3 and anti-CD28. Do you have a detailed protocol for this?

Yes, the detailed protocol is part of our 4D-Nucleofector® Protocol for stimulated Human T Cells.For optimal stimulation of the transfected cells we recommend coating 96-wells plates for stimulation [Nunc Immuno™ Plate C96 Maxi Sorp™, Cat. No. 430...
Missing: mycoalert

What media should be used for spheroid formation with hepatocytes?

Lonza's QC uses HCM (CC-3198) supplemented with 20% FBS and 25 mM Hepes. No plating media is necessary when seeding for spheroids. 
Missing: mycoalert

How many culture/trans-wells does your B-ALI™ Media Kit kit provide?

1 Ampoule of Normal Human Bronchial Epithelial Cells (NHBE) + 1 B-ALI™ Media Kit will make one 24-well plate for at least 1 month of culture & cells will display beating cilia within 25 days.
Missing: mycoalert

How do I promote keratinocyte terminal differentiation towards corneocytes?

Keratinocyte differentiation can be induced by increasing the concentration of calcium and/or by adding serum to the media. Cells should be initially plated using the standard culture media and conditions and allowed ample time to recover from...
Missing: mycoalert

Can I take the ToxiLight™ Non-Destructive Cytotoxicity BioAssay Kit to look at the kinetics of cell death?

Yes, by taking a sample of the culture media (usually 20µl) into a fresh luminometer plate this can be assayed with the Adenylate Kinase Detection Reagent supplied in the Toxilight kit to look for increased levels of AK over the control. The rest...
Missing: mycoalert
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