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Protocol for optimizing culture conditions for ex vivo activation during CRISPR-Cas9 gene editing in human hematopoietic stem and progenitor cells
Long-range correction strategies require ex vivo activation of hematopoietic stem and progenitor cells (HSPCs) to engage the homology-directed repair (HDR) mechanism, but prolonged culture causes harmful cellular responses, reducing the long-term...
Authors:
Della Volpe L, Vacca R, Di Micco R
SST Peer Reviewed
Human Stellate Cell Growth Media
MCST250 is a medium optimized for the culture of human hepatic stellate cells. Storage = - 20 ºC (shelf life is 6 month from date of manufacture)Storage = 2ºC to 8ºC (shelf life is 4 weeks after thawing)
Dental Pulp Stem Cell Media BulletKit
Dental Pulp Stem Cell Media Bulletkit was developped to Dental Pulp derived Mesenchymal Stem Cells without inducing differentiation.
A p38 MAPK-ROS axis fuels proliferation stress and DNA damage during CRISPR-Cas9 gene editing in hematopoietic stem and progenitor cells
Ex vivo activation is a prerequisite to reaching adequate levels of gene editing by homology-directed repair (HDR) for hematopoietic stem and progenitor cell (HSPC)-based clinical applications. Here, we show that shortening culture time mitigates the...
Authors:
Della Volpe L, Midena F, Vacca R, Tavella T, Alessandrini L, Farina G, Brandas C, Lo Furno E,...
SST Peer Reviewed
Hypoimmunogenic Human Pluripotent Stem Cells as a Powerful Tool for Liver Regenerative Medicine
Induced pluripotent stem cells (iPSC) have huge potential as cell therapy for various diseases, given their potential for unlimited self-renewal and capability to differentiate into a wide range of cell types. Although autologous iPSCs represents the...
Authors:
Piera Trionfini , Elena Romano , Marco Varinelli , Lorena Longaretti , Paola Rizzo , Roberta...
Near-perfect precise on-target editing of human hematopoietic stem and progenitor cells
Precision gene editing in primary hematopoietic stem and progenitor cells (HSPCs) would facilitate both curative treatments for monogenic disorders as well as disease modelling. Precise efficiencies even with the CRISPR/Cas system, however, remain...
Authors:
Cloarec-Ung FM, Beaulieu J, Suthananthan A, Lehnertz B, Sauvageau G, Sheppard HM, Knapp DJHF
SST Peer Reviewed
eCHO™ Medium and Feed
eCHO™ Basal Medium and Feed is a chemically defined, hydrolysate-free, serum-free, and non-animal origin medium for supporting high-density CHO cells in suspension.Improved cell growth and protein yield are achieved when adding the...
Efficient prime editing in mouse brain, liver and heart with dual AAVs
Realizing the promise of prime editing for the study and treatment of genetic disorders requires efficient methods for delivering prime editors (PEs) in vivo. Here we describe the identification of bottlenecks limiting adeno-associated virus...
Authors:
Jessie R. Davis, Samagya Banskota, Jonathan M. Levy, Gregory A. Newby, Xiao Wang, Andrew V....
TheraPeak® T-VIVO®
TheraPEAK® T-VIVO® Cell Culture Medium is a chemically-defined, non-animal origin (NAO) cell culture medium to culture and grow a variety of hematopoietic cells including:T cells: both dd (Gamma Delta) and aß (Alpha Beta) subpopulationsCAR-T...
TheraPro® CHO Media System
The TheraPRO® CHO Media System is a chemically defined, animal component-free formulation proven to optimize productivity and product quality when used in conjunction with the GS-CHO cell line.TheraPRO® CHO Media System is made of 4 components which...
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