Search Results

Global organelle profiling reveals subcellular localization and remodeling at proteome scale

Defining the subcellular distribution of all human proteins and their remodeling across cellular states remains a central goal in cell biology. Here, we present a high-resolution strategy to map subcellular organization using organelle immunocapture...

Authors: Hein MY, Peng D, Todorova V, McCarthy F, Kim K, Liu C, Savy L, Januel C, Baltazar-Nunez R, Sekhar M,...

SST Peer Reviewed

T cell gene targeting and selection using non-viral intron knockins

The ability to insert new DNA sequences into defined sites in the genome of human T cells has facilitated more powerful basic research technologies and the engineering of clinical cell therapies. However, current methods target exonic regions, which...

Authors: Roth TL, Lu J, McClellan A, Kernick C, Takacsi-Nagy O, Satpathy AT

SST Peer Reviewed

An efficient, non-viral arrayed CRISPR screening platform for iPSC-derived myeloid and microglia models

Here, we developed a CRISPR-Cas9 arrayed screen to investigate lipid handling pathways in human induced pluripotent stem cell (iPSC)-derived microglia. We established a robust method for the nucleofection of CRISPR-Cas9 ribonucleoprotein complexes...

Authors: Sonja Meier, Anne Sofie Gry Larsen, Florian Wanke, Nicolas Mercado, Arianna Mei, Livia Takacs, Eva...

SST Peer Reviewed

Improving prime editing with an endogenous small RNA-binding protein

Prime editing enables the precise modification of genomes through reverse transcription of template sequences appended to the 3' ends of CRISPR-Cas guide RNAs1. To identify cellular determinants of prime editing, we developed scalable prime editing...

Authors: Yan J, Oyler-Castrillo P, Ravisankar P, Ward CC, Levesque S, Jing Y, Simpson D, Zhao A, Li H, Yan W,...

SST Peer Reviewed

Engineering tandem CD33xCD146 CAR CIK (cytokine-induced killer) cells to target the acute myeloid leukemia niche

In acute myeloid leukemia (AML), malignant stem cells hijack the normal bone marrow niche where they are largely protected from the current therapeutic approaches. Thus, eradicating these progenitors is the ultimate challenge in the treatment of this...

Authors: Gaia Alberti, Corinne Arsuffi, Alice Pievani, Domenico Salerno, Francesco Mantegazza, Francesco...

DNA replication dynamics during erythrocytic schizogony in the malaria parasites Plasmodium falciparum and Plasmodium knowlesi

Malaria parasites are unusual, early-diverging protozoans with non-canonical cell cycles. They do not undergo binary fission, but divide primarily by schizogony. This involves the asynchronous production of multiple nuclei within the same cytoplasm,...

Authors: Jennifer McDonald, Catherine J. Merrick

Fate induction in CD8 CAR T cells through asymmetric cell division

Early expansion and long-term persistence predict efficacy of chimeric antigen receptor T cells (CARTs)1-7, but mechanisms governing effector versus memory CART differentiation and whether asymmetric cell division induces differential fates in human...

Authors: Lee CS, Chen S, Berry CT, Kelly AR, Herman PJ, Oh S, O'Connor RS, Payne AS, Ellebrecht CT

SST Peer Reviewed

Validation of the monocyte activation test with three therapeutic monoclonal antibodies

Pharmaceutical products intended for parenteral use must be free from pyrogenic (fever-inducing) contamination. Pyrogens comprise endotoxins from Gram-negative bacteria and non-endotoxin pyrogens from Gram-positive bacteria, viruses, and fungi. The...

Authors: Ruth Daniels, Wim Van der Elst, Nele Dieltjens, Tinne Appels, Chi K So, Thomas Nys, Liesbeth...

SST Peer Reviewed

Generation of a Transgenic Plasmodium cynomolgi Parasite Expressing Plasmodium vivax Circumsporozoite Protein for Testing P. vivax CSP-Based Malaria Vaccines in Non-Human Primates

Background/Objectives: Malaria, caused by infection with Plasmodium parasites, exacts a heavy toll worldwide. There are two licensed vaccines for malaria as well as two monoclonal antibodies that have shown promising efficacy in field...

Authors: Maya Aleshnick, Shreeya Hegde, Charlie Jennison, Sebastian A. Mikolajczak, Ashley M....

Brain-Wide Transgene Expression in Mice by Systemic Injection of Genetically Engineered Exosomes: CAP-Exosomes

The bottleneck in drug discovery for central nervous system diseases is the absence of effective systemic drug delivery technology for delivering therapeutic drugs into the brain. Despite the advances in the technology used in drug discovery, such as...

Authors: Sarkar SN, Corbin D, Simpkins JW

SST Peer Reviewed

Data Type

Category

Research Area

Platform

Topic

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Characteristic

Format

Origin

Global organelle profiling reveals subcellular localization and remodeling at proteome scale

T cell gene targeting and selection using non-viral intron knockins

An efficient, non-viral arrayed CRISPR screening platform for iPSC-derived myeloid and microglia models

Improving prime editing with an endogenous small RNA-binding protein

Engineering tandem CD33xCD146 CAR CIK (cytokine-induced killer) cells to target the acute myeloid leukemia niche

DNA replication dynamics during erythrocytic schizogony in the malaria parasites Plasmodium falciparum and Plasmodium knowlesi

Fate induction in CD8 CAR T cells through asymmetric cell division

Validation of the monocyte activation test with three therapeutic monoclonal antibodies

Generation of a Transgenic Plasmodium cynomolgi Parasite Expressing Plasmodium vivax Circumsporozoite Protein for Testing P. vivax CSP-Based Malaria Vaccines in Non-Human Primates

Brain-Wide Transgene Expression in Mice by Systemic Injection of Genetically Engineered Exosomes: CAP-Exosomes