Search Results

Harnessing noncanonical crRNA for highly efficient genome editing

The CRISPR-Cas12a system is more advantageous than the widely used CRISPR-Cas9 system in terms of specificity and multiplexibility. However, its on-target editing efficiency is typically much lower than that of the CRISPR-Cas9 system. Here we...

Authors: Xun G, Zhu Z, Singh N, Lu J, Jain PK, Zhao H

SST Peer Reviewed

Efficient hepatocyte differentiation of primary human hepatocyte-derived organoids using three dimensional nanofibers (HYDROX) and their possible application in hepatotoxicity research

Human liver organoids are in vitro three dimensionally (3D) cultured cells that have a bipotent stem cell phenotype. Translational research of human liver organoids for drug discovery has been limited by the challenge of their low hepatic function...

Authors: Yanran Tong, Yukiko Ueyama-Toba, Jumpei Yokota, Hayato Matsui, Masaki Kanai & Hiroyuki Mizuguchi

Transfection of choanoflagellates illuminates their cell biology and the ancestry of animal septins

As the closest living relatives of animals, choanoflagellates offer unique insights into animal origins and core mechanisms underlying animal cell biology. However, unlike traditional model organisms, such as yeast, flies, and worms,...

Authors: David S Booth , Heather Szmidt-Middleton , Nicole King

Targeting P4HA1 promotes CD8+ T cell progenitor expansion toward immune memory and systemic anti-tumor immunity

Successful immunotherapy relies on both intratumoral and systemic immunity, which is yet to be achieved for most patients with cancer. Here, we identify P4HA1, encoding prolyl 4-hydroxylase 1, as a crucial regulator of CD8+ T cell...

Authors: Ma S, Ong LT, Jiang Z, Lee WC, Lee PL, Yusuf M, Ditzel HJ, Wang Y, Chen Q, Wang W, Wu X, Tan EY, Yu...

SST Peer Reviewed

SICA-mediated cytoadhesion of Plasmodium knowlesi-infected red blood cells to human umbilical vein endothelial cells

Zoonotic malaria due to Plasmodium knowlesi infection in Southeast Asia is sometimes life-threatening. Post-mortem examination of human knowlesi malaria cases showed sequestration of P. knowlesi-infected red blood cells...

Authors: Huai Chuang, Miako Sakaguchi, Amuza Byaruhanga Lucky, Junya Yamagishi, Yuko Katakai, Satoru...

Automated CRISPR/Cas9-based genome editing of human pluripotent stem cells using the StemCellFactory

CRISPR/Cas9 genome editing is a rapidly advancing technology that has the potential to accelerate research and development in a variety of fields. However, manual genome editing processes suffer from limitations in scalability, efficiency, and...

Authors: Bastian Nießing, Yannik Breitkreuz , Andreas Elanzew , Marcelo A S de Toledo , Peter Vajs , Marina...

Genome editing enables reverse genetics of multicellular development in the choanoflagellate Salpingoeca rosetta

In a previous study, we established a forward genetic screen to identify genes required for multicellular development in the choanoflagellate, Salpingoeca rosetta (Levin et al., 2014). Yet, the paucity of reverse genetic tools for choanoflagellates...

Authors: David S Booth , Nicole King

Genome-wide CRISPR screens identify critical targets to enhance CAR-NK cell antitumor potency

Adoptive cell therapy using engineered natural killer (NK) cells is a promising approach for cancer treatment, with targeted gene editing offering the potential to further enhance their therapeutic efficacy. However, the spectrum of...

Authors: Biederstädt A, Basar R, Park JM, Uprety N, Shrestha R, Reyes Silva F, Dede M, Watts J, Acharya S,...

SST Peer Reviewed

STING mediates immune responses in the closest living relatives of animals

Animals have evolved unique repertoires of innate immune genes and pathways that provide their first line of defense against pathogens. To reconstruct the ancestry of animal innate immunity, we have developed the choanoflagellate Monosiga...

Authors: Arielle Woznica , Ashwani Kumar , Carolyn R Sturge , Chao Xing , Nicole King , Julie K Pfeiffer

CRISPR/Cas9 Ribonucleoprotein Nucleofection for Genome Editing in Primary Human Keratinocytes: Knockouts, Deletions, and Homology-Directed Repair Mutagenesis

Keratinocytes are the most abundant cell type in the human epidermis, the outermost layer of the skin. For years, primary human keratinocytes (HKs) have been used as a crucial tool for studying the pathogenesis of a wide range of skin-related...

Authors: Bamundo M, Palumbo S, D'Auria L, Missero C, Di Girolamo D

SST Peer Reviewed

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Harnessing noncanonical crRNA for highly efficient genome editing

Efficient hepatocyte differentiation of primary human hepatocyte-derived organoids using three dimensional nanofibers (HYDROX) and their possible application in hepatotoxicity research

Transfection of choanoflagellates illuminates their cell biology and the ancestry of animal septins

Targeting P4HA1 promotes CD8+ T cell progenitor expansion toward immune memory and systemic anti-tumor immunity

SICA-mediated cytoadhesion of Plasmodium knowlesi-infected red blood cells to human umbilical vein endothelial cells

Automated CRISPR/Cas9-based genome editing of human pluripotent stem cells using the StemCellFactory

Genome editing enables reverse genetics of multicellular development in the choanoflagellate Salpingoeca rosetta

Genome-wide CRISPR screens identify critical targets to enhance CAR-NK cell antitumor potency

STING mediates immune responses in the closest living relatives of animals

CRISPR/Cas9 Ribonucleoprotein Nucleofection for Genome Editing in Primary Human Keratinocytes: Knockouts, Deletions, and Homology-Directed Repair Mutagenesis