Search Results

Variant surface protein GP60 contributes to host infectivity of Cryptosporidium parvum

Biological studies of the determinants of Cryptosporidium infectivity are lacking despite the fact that cryptosporidiosis is a major public health problem. Recently, the 60-kDa glycoprotein (GP60) has received attention because of its high...

Authors: Muxiao Li, Fuxian Yang, Tianyi Hou, Xiaoqing Gong, Na Li, L. David Sibley, Yaoyu Feng, Lihua...

Systematic screens for fertility genes essential for malaria parasite transmission reveal conserved aspects of sex in a divergent eukaryote

Sexual reproduction in malaria parasites is essential for their transmission to mosquitoes and offers a divergent eukaryote model to understand the evolution of sex. Through a panel of genetic screens in Plasmodium berghei, we identify 348 sex...

Authors: Claire Sayers, Vikash Pandey, Arjun Balakrishnan, Katharine Michie, Dennis Svedberg, Mirjam...

A CRISPR homing screen finds a chloroquine resistance transporter-like protein of the Plasmodium oocyst essential for mosquito transmission of malaria

Genetic screens with barcoded PlasmoGEM vectors have identified thousands of Plasmodium berghei gene functions in haploid blood stages, gametocytes and liver stages. However, the formation of diploid cells by fertilisation has hindered...

Authors: Arjun Balakrishnan, Mirjam Hunziker, Puja Tiwary, Vikash Pandey, David Drew , Oliver Billker

A High-Throughput Microphysiological Liver Chip System to Model Drug-Induced Liver Injury Using Human Liver Organoids

Background and AimsDrug-induced liver injury (DILI) is a major failure mode in pharmaceutical development. This study aims to address the limitations of existing preclinical models by assessing a high-throughput, microfluidic liver-on-a-chip system,...

Authors: Sophia R. Meyer , Charles J. Zhang , Max A. Garcia, Megan C. Procario , Sanghee Yoo , Amber...

An optimized SpCas9 high-fidelity variant for direct protein delivery

Electroporation of the Cas9 ribonucleoprotein (RNP) complex offers the advantage of preventing off-target cleavages and potential immune responses produced by long-term expression of the nuclease. Nevertheless, the majority of engineered...

Authors: Pedrazzoli E, Bianchi A, Umbach A, Amistadi S, Brusson M, Frati G, Ciciani M, Badowska KA, Arosio D,...

SST Peer Reviewed

Human iPSC-derived liver co-culture spheroids to model liver fibrosis

The lack of adequate humanin vitromodels that recapitulate the cellular composition and response of the human liver to injury hampers the development of anti-fibrotic drugs. The goal of this study was to develop a human spheroid culture model to...

Authors: Laura Cools , Mina Kazemzadeh Dastjerd , Ayla Smout , Vincent Merens , Yuwei Yang , Hendrik...

Phosphoglycolate phosphatase is a metabolic proofreading enzyme essential for cellular function in Plasmodium berghei

Plasmodium falciparum (Pf) 4-nitrophenylphosphatase has been shown previously to be involved in vitamin B1 metabolism. Here, conducting a BLASTp search, we found that 4-nitrophenylphosphatase from Pf has significant homology with...

Authors: Lakshmeesha Kempaiah Nagappa, Pardhasaradhi Satha, Thimmaiah Govindaraju, Hemalatha Balaram

Orthogonal transcriptional modulation and gene editing using multiple CRISPR-Cas systems

CRISPR-Cas-based transcriptional activation (CRISPRa) and interference (CRISPRi) enable transient programmable gene regulation by recruitment or fusion of transcriptional regulators to nuclease-deficient Cas (dCas). Here, we expand on...

Authors: Broksø AD, Bendixen L, Fammé S, Mikkelsen K, Jensen TI, Bak RO

SST Peer Reviewed

Efficient hepatocyte differentiation of primary human hepatocyte-derived organoids using three dimensional nanofibers (HYDROX) and their possible application in hepatotoxicity research

Human liver organoids are in vitro three dimensionally (3D) cultured cells that have a bipotent stem cell phenotype. Translational research of human liver organoids for drug discovery has been limited by the challenge of their low hepatic function...

Authors: Yanran Tong, Yukiko Ueyama-Toba, Jumpei Yokota, Hayato Matsui, Masaki Kanai & Hiroyuki Mizuguchi

Merozoite surface protein 1 paralog is involved in the human erythrocyte invasion of a zoonotic malaria, Plasmodium knowlesi

The zoonotic malaria parasite Plasmodium knowlesi is an important public health concern in Southeast Asia. Invasion of host erythrocytes is essential for parasite growth, and thus, understanding the repertoire of parasite proteins that...

Authors: Seong-Kyun Lee, Tuyet Kha Nguyen, Franziska Mohring, Jin-Hee Han, Egy Rahman Firdaus, Sung-Hun...

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