Search Results

A microvascularized in vitro liver model for disease modeling and drug discovery

Drug discovery for complex liver diseases faces alarming attrition rates. The lack of non-clinical models that recapitulate key aspects of liver (patho)-physiology is likely contributing to the inefficiency of developing effective...

Authors: Flavio Bonanini, Roelof Dinkelberg, Manuel Caro Torregrosa , Nienke Kortekaas , Tessa M. S.

Optimizing the procedure for manufacturing clinical-grade genetically manipulated natural killer cells for adoptive immunotherapy

Background aims: Ex vivo-expanded natural killer (NK) cells hold significant potential as antitumor effector cells for adoptive immunotherapy. However, producing clinical-grade, genetically modified NK cells in sufficient quantities presents a...

Authors: Lin H, Ye S, Zhang S, Ge T, Li D, Huang L, Zhu L, Mu W

SST Peer Reviewed

Naturally occurring T cell mutations enhance engineered T cell therapies

Adoptive T cell therapies have produced exceptional responses in a subset of patients with cancer. However, therapeutic efficacy can be hindered by poor T cell persistence and function1. In human T cell cancers, evolution of the disease positively...

Authors: Garcia J, Daniels J, Lee Y, Zhu I, Cheng K, Liu Q, Goodman D, Burnett C, Law C, Thienpont C, Alavi...

SST Peer Reviewed

Optimization of a DiCre recombinase system with reduced leakage for conditional genome editing of Cryptosporidium

The dimerizable Cre recombinase system (DiCre) exhibits increased leaky activity in Cryptosporidium, leading to unintended gene editing in the absence of induction. Therefore, optimization of the current DiCre technique is necessary for...

Authors: Yue Huang, Jinli Li, Shifeng Pei, Heng You, Huimin Liu, Yaqiong Guo, Rui Xu, Na Li, Yaoyu...

Human CD34+-derived plasmacytoid dendritic cells as surrogates for primary pDCs and potential cancer immunotherapy

Introduction: Plasmacytoid dendritic cells (pDCs) are capable of triggering broad immune responses, yet, their scarcity in blood coupled to their reduced functionality in cancer, makes their therapeutic use for in situ activation or...

Authors: Fiore G, Weckwarth W, Paetzold K, Albertí Servera L, Gies M, Rosenhauer J, Antoniolli M, Nassiri S,...

SST Peer Reviewed

Gene-editing in patient and humanized-mice primary muscle stem cells rescues dysferlin expression in dysferlin-deficient muscular dystrophy

Dystrophy-associated fer-1-like protein (dysferlin) conducts plasma membrane repair. Mutations in the DYSF gene cause a panoply of genetic muscular dystrophies. We targeted a frequent loss-of-function, DYSF exon 44, founder frameshift mutation with...

Authors: Escobar H, Di Francescantonio S, Smirnova J, Graf R, Müthel S, Marg A, Zhogov A, Krishna S, Metzler...

SST Peer Reviewed

Organoid-based in vitro system and reporter for the study of Cryptosporidium parvum sexual reproduction

Many advances have been made recently in our understanding of Cryptosporidium’s asexual cycle and sexual differentiation. However, the process of fertilization, which is required for transmission of infectious oocysts, is not well understood. Typical...

Authors: Bethany R. Korwin-Mihavics, Emmett A. Dews, Peter Miller, Alexandra Cameron, Bruno Martorelli di...

Molecular and Physiological Effects of Browning Agents on White Adipocytes from Bone Marrow Mesenchymal Stromal Cells

Two different types of adipose depots can be observed in mammals: white adipose tissue (WAT) and brown adipose tissue (BAT). The primary role of WAT is to deposit surplus energy in the form of triglycerides, along with many metabolic and hormonal...

Authors: Girolamo Di Maio, Nicola Alessio, Gianfranco Peluso, Silverio Perrotta, Marcellino Monda, Giovanni...

Manufacturing NKG2D CAR-T cells with piggyBac transposon vectors and K562 artificial antigen-presenting cells

Non-viral platforms can be applied rapidly and cost-effectively for chimeric antigen receptor (CAR)-T cell manufacturing. In the present paper, we describe in detail a clinically relevant manufacturing process for NKG2D CAR-T cells through...

Authors: Tay JCK, Wang J, Du Z, Ng YY, Li Z, Ren Y, Zhang C, Zhu J, Xu XH, Wang S

SST Peer Reviewed

Protocol to create phenotypic primary human hepatocyte cultures using the RASTRUM 3D cell model platform

To improve human hepatotoxicity prediction, in vitro liver cell models replicating hepatocyte function, drug metabolism, and toxicity are required. Here, we present a protocol for creating 3D primary human hepatocyte (PHH) cell models using the...

Authors: Christine Yee , Yik Lung Chan , Robert Utama , Marie Besnier , Martin Engel , Lisa Belfiore

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A microvascularized in vitro liver model for disease modeling and drug discovery

Optimizing the procedure for manufacturing clinical-grade genetically manipulated natural killer cells for adoptive immunotherapy

Naturally occurring T cell mutations enhance engineered T cell therapies

Optimization of a DiCre recombinase system with reduced leakage for conditional genome editing of Cryptosporidium

Human CD34+-derived plasmacytoid dendritic cells as surrogates for primary pDCs and potential cancer immunotherapy

Gene-editing in patient and humanized-mice primary muscle stem cells rescues dysferlin expression in dysferlin-deficient muscular dystrophy

Organoid-based in vitro system and reporter for the study of Cryptosporidium parvum sexual reproduction

Molecular and Physiological Effects of Browning Agents on White Adipocytes from Bone Marrow Mesenchymal Stromal Cells

Manufacturing NKG2D CAR-T cells with piggyBac transposon vectors and K562 artificial antigen-presenting cells

Protocol to create phenotypic primary human hepatocyte cultures using the RASTRUM 3D cell model platform