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The PCH Family Member Proline-Serine-Threonine Phosphatase-interacting Protein 1 Targets to the Leukocyte Uropod and Regulates Directed Cell Migration

PCH family members have emerged as important regulators of membrane-cytoskeletal interactions. Here we show that PSTPIP1, a PCH family member expressed in hematopoietic cells, regulates the motility of neutrophil-like cells and is a novel component...

Authors: Cooper KM, Bennin DA, Huttenlocher A

High-level transfection of primary rabbit T lymphocytes

Efficient gene delivery is essential for the assessment of transgene function in untransformed hematopoietic cells. Here, we explored the utility of different non-viral and viral gene delivery techniques for primary T cells from New Zealand White...

Authors: Tervo HM, Allespach I, Keppler OT

SST Peer Reviewed

Pim kinases promote cell cycle progression by phosphorylating and down-regulating p27Kip1 at the transcriptional and posttranscriptional levels

The serine/threonine kinase Pim is known to promote cell cycle progression and to inhibit apoptosis leading to tumorigenesis. However, the precise mechanisms remain unclear. We show, herein, that all the Pim family members (Pim1, Pim2, and Pim3) bind...

Authors: Morishita D, Katayama R, Sekimizu K, Tsuruo T, Fujita N

miR-206 Expression is down-regulated in estrogen receptor alpha-positive human breast cancer

Expression levels of estrogen receptor (ER) alpha govern estrogen-dependent growth, response to endocrine therapy, and prognosis in ERalpha-positive breast cancer. Multiple mechanisms involved in altering ERalpha gene expression in breast cancer have...

Authors: Kondo N, Toyama T, Sugiura H, Fujii Y, Yamashita H

The warburg effect in leukemia-stroma cocultures is mediated by mitochondrial uncoupling associated with uncoupling protein 2 activation

In 1956, Otto Warburg proposed that the origin of cancer cells was closely linked to a permanent respiratory defect that bypassed the Pasteur effect (i.e., the inhibition of anaerobic fermentation by oxygen). Since then, permanent defects in oxygen...

Authors: Samudio I, Fiegl M, McQueen T, Clise-Dwyer K, Andreeff M

RSV-induces RelA release from cytoplasmic 100 kDa NF-kappa B2 complexes via a novel retinoic acid inducible gene-I-NF-kappa B inducing kinase (NIK) signaling pathway

Respiratory Syncytial Virus (RSV) is a primary cause of severe lower respiratory tract infection in children worldwide. RSV infects airway epithelial cells, where it activates inflammatory genes via the NF-B pathway. NF-B is controlled by two...

Authors: Liu P, Li K, Garofalo RP, Brasier AR

Signaling through ephrin-A ligand leads to activation of Src-family kinases, Akt phosphorylation, and inhibition of antigen receptor-induced apoptosis

Eph receptor tyrosine kinases and ephrins play important roles in diverse biological processes such as migration, adhesion, and angiogenesis. Forward and reverse signaling has been reported in receptor- and ligand-bearing cells. The ligands can be...

Authors: Holen HL, Shadidi M, Narvhus K, KjÜ¸snes O, Tierens A, Aasheim HC

PKCdelta acts upstream of SPAK in the activation of NKCC1 by hyperosmomtic stress in airway epithelial cells

Airway epithelial Na-K-2Cl (NKCC1) cotransport is activated through hormonal stimulation and hyperosmotic stress via a PKCdelta-mediated intracellular signalling pathway. Downregulation of PKCdeltaprevents activation of NKCC1 expressed in Calu-3...

Authors: Smith L, Smallwood N, Altman A, Liedtke CM

Orphan nuclear receptor NR4A2 expressed in T cells from multiple sclerosis mediates production of inflammatory cytokines

Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS) mediated by Th17 and Th1 cells. DNA microarray analysis previously showed that NR4A2, an orphan nuclear receptor, is strongly up-regulated in the peripheral blood T...

Authors: Doi Y, Oki S, Ozawa T, Hohjoh H, Miyake S, Yamamura T

STAT5b, c-Src, and EGFR Signaling Play Integral Roles in Estrogen-Stimulated Proliferation of ER-Positive Breast Cancer Cells

17beta-estradiol (E2) acts through the estrogen receptor alpha (ERalpha) to stimulate breast cancer proliferation. Here, we investigated the functional relationship between ERalpha and STAT5b activity in ER+ MCF-7 and T47D human breast cancer cells...

Authors: Fox EM, Bernaciak TM, Wen J, Weaver AM, Shupnik MA, Silva CM

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