Search Results

Can cell free supernatants ( NOT lysed) be stored for later testing with ViaLight™ Plus BioAssay?

For supernatants there will be exogenous ATPases present that will degrade ATP so it should really be treated as if it were cells. Freeze as quickly as possible and store at -80C, for no more than 4 weeks. The ATP will be OK with the freezing.

What factors interfere with the ViaLight™ Plus Assay?

Certain compounds will quench the light emission in bioluminescent reactions. Typically these are strong red dyes (however, the ViaLight ™ Plus Assay has been designed to function in all phenol red containing media commonly used in...

For Lonza’s MycoAlert® Mycoplasma Detection Assays is it possible to convert the B/A value of positive control included in the MycoAlert® Assay Control Set to CFU of mycoplasma?

The MycoAlert® and MycoAlert® PLUS Assay are not quantitative so you cannot relate the ratio to an actual value. Different mycoplasma species will give different ratios due to different levels of expression of the enzymes we measure -...

What are inhibitors of the MycoAlert® Mycoplasma Detection Kit

The main inhibitors of the MycoAlert® Reaction would be things that affect the luciferase detection. Agents which potentially quench the light signal include high levels of salt, EDTA and other chelating agents, very high levels of sera,...

Do you have recommendations for the type of samples used for the MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Detection Kit? And do you have recommendations related to sample storage?

The MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Assay require the use of cell free supernatant only! Cells MUST be removed at 200xg for 5 mins.For optimal assay performance, supernatant should be tested...

Do antibiotics effect the MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits?

Gentamycin and all commonly used antiboitics do not effect MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Assay. We have tested amphotericin, ampicillin, cephalothin, kanamycin, lincomycin, in addition to gentamycin, penicillin and streptomycin...

Can I store my cryopreserved primary cell ampule at –80°C?

No, cryopreserved cells should be stored in liquid or vapor phase nitrogen. Storing the cells at higher temperatures can irreversibly damage the cells.

What is the effect of pH on the Luciferase-based BioAssays (ViaLight™ Plus, MycoAlert®, ToxiLight®) Lonza is offering?

A low pH can certainly affect the luciferase system used in all of these products. The luciferase will not work if the pH is below 6.5 and performance will rapidly decay below pH 7.2. The reagents in these kits will buffer back to some degree but...

How do you calculate a population doubling?

A population doubling is the total number of population doublings of a cell type since its initiation in vitro. The number of population doubling is calculated using the following equations:Cell count at day of passage /Cell count 24 hours after...

How can cells be cured of mycoplasma contamination?

Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™ Mycoplasma Elimination Reagent has been optimized to eliminate mycoplasma with minimal toxic effects on the cells.The MycoZap™ Reagent eliminates mycoplasma...

Data Type

Category

Research Area

Platform

Topic

Species

Characteristic

Format

Origin