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Liver organoids: from basic research to therapeutic applications

Organoid cultures have emerged as an alternative in vitro system to recapitulate tissues in a dish. While mouse models and cell lines have furthered our understanding of liver biology and associated diseases, they suffer in replicating key aspects of...

Authors: Nicole Prior , Patricia Inacio , Meritxell Huch

Apicoplast ribosomal protein S10-V127M enhances artemisinin resistance of a Kelch13 transgenic Plasmodium falciparum

Background: The resistance of Plasmodium falciparum to artemisinin-based (ART) drugs, the front-line drug family used in artemisinin-based combination therapy (ACT) for treatment of malaria, is of great concern. Mutations in the kelch13 (k13)...

Authors: Tanyaluck Kampoun, Somdet Srichairatanakool, Parichat Prommana, Philip J Shaw, Judith L...

In vitro characterization of 3D culture-based differentiation of human liver stem cells

Introduction: The lack of functional hepatocytes poses a significant challenge for drug safety testing and therapeutic applications due to the inability of mature hepatocytes to expand and their tendency to lose functionality in vitro....

Authors: Marta Tapparo , Gabriele Saccu , Chiara Pasquino , Valentina Fonsato , Claudio Medana , Valentina...

Deletion of Plasmodium falciparum ubc13 increases parasite sensitivity to the mutagen, methyl methanesulfonate and dihydroartemisinin

The inducible Di-Cre system was used to delete the putative ubiquitin-conjugating enzyme 13 gene (ubc13) of Plasmodium falciparum to study its role in ubiquitylation and the functional consequence during the parasite asexual blood...

Authors: Supawadee Maneekesorn, Ellen Knuepfer, Judith L. Green, Parichat Prommana, Chairat...

Adaptation of Plasmodium falciparum to humans involved the loss of an ape-specific erythrocyte invasion ligand

Plasmodium species are frequently host-specific, but little is currently known about the molecular factors restricting host switching. This is particularly relevant for P. falciparum, the only known human-infective species of...

Authors: William R. Proto, Sasha V. Siegel, Selasi Dankwa, Weimin Liu, Alison Kemp, Sarah Marsden,Zenon A....

Long-term HBV infection of engineered cultures of induced pluripotent stem cell-derived hepatocytes

Background: HBV infects ~257 million people and can cause hepatocellular carcinoma. Since current drugs are not curative, novel therapies are needed. HBV infects chimpanzee and human livers. However, chimpanzee studies are severely restricted...

Authors: Yang Yuan , Vedant V Bodke , Christine Lin , Shang Gao , Jalees Rehman , Jisu Li , Salman R...

PfFBXO1 is essential for inner membrane complex formation in Plasmodium falciparum during both asexual and transmission stages

lasmodium species replicate via schizogony, which involves asynchronous nuclear divisions followed by semi-synchronous segmentation and cytokinesis. Successful segmentation requires a double-membranous structure known as the inner membrane...

Authors: Sreelakshmi K. Sreenivasamurthy, Carlos Gustavo Baptista, Christopher M. West, Ira J....

A solution for highly efficient electroporation of primary cytotoxic T lymphocytes

Background: Cytotoxic T lymphocytes (CTLs) are central players in the adaptive immune response. Their functional characterization and clinical research depend on efficient and reliable transfection. Although various methods have been utilized,...

Authors: Alawar N, Schirra C, Hohmann M, Becherer U

SST Peer Reviewed

C-JUN overexpressing CAR-T cells in acute myeloid leukemia: preclinical characterization and phase I trial

Chimeric antigen receptor (CAR) T cells show suboptimal efficacy in acute myeloid leukemia (AML). We find that CAR T cells exposed to myeloid leukemia show impaired activation and cytolytic function, accompanied by impaired antigen receptor...

Authors: Zuo S, Li C, Sun X, Deng B, Zhang Y, Han Y, Ling Z, Xu J, Duan J, Wang Z, Yu X, Zheng Q, Xu X, Zong...

SST Peer Reviewed

A scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite Plasmodium berghei

Many Plasmodium genes remain uncharacterized due to low genetic tractability. Previous large-scale knockout screens have only been able to target about half of the genome in the more genetically tractable rodent malaria...

Authors: Thorey K Jonsdottir, Martina S Paoletta, Takahiro Ishizaki, Sophia Hernandez, Maria Ivanova, Alicia...

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