Search Results

Generating and maintaining transgenic Cryptosporidium parvum parasites

The apicomplexan parasite Cryptosporidium is a leading cause of diarrheal disease and an important contributor to overall global child mortality. We currently lack effective treatment and immune prophylaxis. Recent advances now permit...

Authors: Mattie C. Pawlowic, Sumiti Vinayak, Adam Sateriale, Carrie F. Brooks, Boris Striepen

Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms

The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a grave threat to public health and the global economy. SARS-CoV-2 is closely related to the more lethal but less transmissible coronaviruses SARS-CoV-1...

Authors: David E. Gordon*, Joseph Hiatt*, Mehdi Bouhaddou*, Veronica V. Rezelj*, Svenja Ulferts*, Hannes...

SST Peer Reviewed

A Genetically Tractable, Natural Mouse Model of Cryptosporidiosis Offers Insights into Host Protective Immunity

Cryptosporidium is a leading cause of diarrheal disease and an important contributor to early childhood mortality, malnutrition, and growth faltering. Older children in high endemicity regions appear resistant to infection, while...

Authors: Adam Sateriale, Jan Šlapeta, Rodrigo Baptista, Julie B. Engiles, Jodi A. Gullicksrud,...

Protocol for genomic editing in human resting primary NK cells and NK-92 cells via CRISPR-Cas9 ribonucleoproteins

Here, we present a protocol to perform CRISPR-Cas9 genome editing in human resting primary natural killer (NK) and NK-92 cells. We describe steps for guide RNA selection, ribonucleoprotein (RNP) complex formation, delivery via Nucleofection, and...

Authors: Verhezen T, Lau HW, Van Audenaerde J, Wouters A, Smits E, De Waele J

SST Peer Reviewed

Advanced three-dimensional in vitro liver models to study the activity of anticancer drugs

The liver is one of the most important organs in the human body. It performs many important functions, including being responsible for the metabolism of most drugs, which is often associated with its drug-induced damage. Currently, there are no ideal...

Authors: Agnieszka Zuchowska , Sonia Frojdenfal , Maciej Trzaskowski , Elzbieta Jastrzebska

Multiple essential functions of Plasmodium falciparum actin-1 during malaria blood-stage development

The phylum Apicomplexa includes intracellular parasites causing immense global disease burden, the deadliest of them being the human malaria parasite Plasmodium falciparum, which invades and replicates within erythrocytes. The cytoskeletal...

Authors: Sujaan Das, Leandro Lemgruber, Chwen L. Tay, Jake Baum, Markus Meissner

GMP-compliant iPS cell lines show widespread plasticity in a new set of differentiation workflows for cell replacement and cancer immunotherapy

Cell therapeutic applications based on induced pluripotent stem cells (iPSCs) appear highly promising and challenging at the same time. Good manufacturing practice (GMP) regulations impose necessary yet demanding requirements for quality and...

Authors: Terheyden-Keighley D, Hühne M, Berger T, Hiller B, Martins S, Gamerschlag A, Sabour D, Meffert A,...

SST Peer Reviewed

A 39-Amino-Acid C-Terminal Truncation of GDV1 Disrupts Sexual Commitment in Plasmodium falciparum

Malaria is a mosquito-borne disease caused by apicomplexan parasites of the genus Plasmodium. Completion of the parasite's life cycle depends on the transmission of sexual stages, the gametocytes, from an infected human host to the mosquito...

Authors: Marta Tibúrcio, Eva Hitz, Igor Niederwieser, Gavin Kelly, Heledd Davies, Christian Doerig, Oliver Bi...

Liver organoids: updates on generation strategies and biomedical applications

The liver is the most important metabolic organ in the body. While mouse models and cell lines have further deepened our understanding of liver biology and related diseases, they are flawed in replicating key aspects of human liver tissue,...

Authors: Sen Liu , Chuanliang Cheng , Liuyang Zhu , Tianyu Zhao , Ze Wang , Xiulin Yi , Fengying...

AutoBioTech-A Versatile Biofoundry for Automated Strain Engineering

The inevitable transition from petrochemical production processes to renewable alternatives has sparked the emergence of biofoundries in recent years. Manual engineering of microbes will not be sufficient to meet the ever-increasing demand for novel...

Authors: Tobias Michael Rosch, Julia Tenhaef, Tim Stoltmann, Till Redeker, Dominic Kösters, Niels...

SST Peer Reviewed

Data Type

Category

Research Area

Platform

Topic

Species

Characteristic

Format

Origin

Generating and maintaining transgenic Cryptosporidium parvum parasites

Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms

A Genetically Tractable, Natural Mouse Model of Cryptosporidiosis Offers Insights into Host Protective Immunity

Protocol for genomic editing in human resting primary NK cells and NK-92 cells via CRISPR-Cas9 ribonucleoproteins

Advanced three-dimensional in vitro liver models to study the activity of anticancer drugs

Multiple essential functions of Plasmodium falciparum actin-1 during malaria blood-stage development

GMP-compliant iPS cell lines show widespread plasticity in a new set of differentiation workflows for cell replacement and cancer immunotherapy

A 39-Amino-Acid C-Terminal Truncation of GDV1 Disrupts Sexual Commitment in Plasmodium falciparum

Liver organoids: updates on generation strategies and biomedical applications

AutoBioTech-A Versatile Biofoundry for Automated Strain Engineering