Search Results

Non-viral expression of chimeric antigen receptors with multiplex gene editing in primary T cells

Efficient engineering of T cells to express exogenous tumor-targeting receptors such as chimeric antigen receptors (CARs) or T-cell receptors (TCRs) is a key requirement of effective adoptive cell therapy for cancer. Genome editing technologies, such...

Authors: Cappabianca Dan, Li Jingling, Zheng Yueting, Tran Cac, Kasparek Kassandra, Mendez Pedro, Thu Ricky,...

SST Peer Reviewed

EFFICIENT COST-EFFECTIVE MANUFACTURE OF A NON-VIRAL TRANSPOSON BASED NOVEL BAFF-CART FOR TREATMENT OF B-CELL CANCERS

CD19 CART cell therapies have shown remarkable efficacy in non-Hodgkin lymphoma. However, durable response has been observed in less than half of treated patients due to epitope loss and T cell exhaustion. To overcome this limitation, we developed a...

Authors: A.Lawrence, S.Kleinsorge-Block, L.Jonart, P.Caimi, R.Parameswaran, J.Reese

SST Peer Reviewed

The PP2A-Integrator-CDK9 axis fine-tunes transcription and can be targeted therapeutically in cancer

Gene expression by RNA polymerase II (RNAPII) is tightly controlled by cyclin-dependent kinases (CDKs) at discrete checkpoints during the transcription cycle. The pausing checkpoint following transcription initiation is primarily controlled by CDK9....

Authors: Vervoort SJ, Welsh SA, Devlin JR, Barbieri E, Knight DA, Offley S, Bjelosevic S, Costacurta M,...

SST Peer Reviewed

Gravity-perfused airway-on-a-chip optimized for quantitative BSL-3 studies of SARS-CoV-2 infection: barrier permeability, cytokine production, immunohistochemistry, and viral load assays

Human microphysiological systems, such as organs on chips, are an emerging technology for modeling human physiology in a preclinical setting to understand the mechanism of action of drugs, to evaluate the efficacy of treatment options for human...

Authors: Shannon L Faley , Niloufar A Boghdeh , David K Schaffer , Eric C Spivey , Farhang Alem , Aarthi...

Integration of ?-deficient CARs into the CD3-zeta gene conveys potent cytotoxicity in T and NK cells

Chimeric antigen receptor (CAR)-redirected immune cells hold significant therapeutic potential for oncology, autoimmune diseases, transplant medicine, and infections. All approved CAR-T therapies rely on personalized manufacturing using...

Authors: Kath J, Franke C, Drosdek V, Du W, Glaser V, Fuster-Garcia C, Stein M, Zittel T, Schulenberg S,...

SST Peer Reviewed

Breast cancer-on-chip for patient-specific efficacy and safety testing of CAR-T cells

Physiologically relevant human models that recapitulate the challenges of solid tumors and the tumor microenvironment (TME) are highly desired in the chimeric antigen receptor (CAR)-T cell field. We developed a breast cancer-on-chip model with an...

Authors: Tengku Ibrahim Maulana , Claudia Teufel, Madalena Cipriano , Julia Roosz , Lisa...

Robust differentiation of NK cells from MSLN.CAR-IL-15–engineered human iPSCs with enhanced antitumor efficacy against solid tumors

Human induced pluripotent stem cells (iPSCs) offer a promising source for chimeric antigen receptor (CAR)–engineered natural killer (NK) products. However, complex iPSC-NK (iNK) manufacturing challenges clinical use. Here,...

Authors: Jiang Q, Yu W, Ma J, Zhao M, Zou J, Mir S, Zhang J, Germain RN, Hassan R.

SST Peer Reviewed

IRF8 regulates efficacy of therapeutic anti-CD20 monoclonal antibodies

Anti-CD20 monoclonal antibodies such as Rituximab, Ofatumumab, and Obinutuzumab are widely used to treat lymphomas and autoimmune diseases. They act by depleting B cells, mainly through Fc-dependent effectors functions. Some patients develop...

Authors: Grzelak L, Roesch F, Vaysse A, Biton A, Legendre R, Porrot F, Commère P-H, Olanchais C, Mouquet H,...

Immunocompetent Intestine-on-Chip Model for Analyzing Gut Mucosal Immune Responses

An advanced intestine-on-chip model recreating epithelial 3D organotypic villus-like and crypt-like structures has been developed. The immunocompetent model includes Human Umbilical Vein Endothelial Cells (HUVEC), Caco-2 intestinal epithelial cells,...

Authors: Adrian Feile , Valentin D Wegner , Martin Raasch , Alexander S Mosig

THP-1 reference data: Proposal of an in vitro branched evolution model for cancer cell lines

THP-1 is a representative leukemia cell line and is registered with four different numbers in JCRB and RIKEN BRC cell banks. However, differences between these four lines remain unclear. In our study, these four THP-1 cell lines, JCRB0112, JCRB0112.1...

Authors: Fumio Kasai1,2 | Noriko Hirayama2 | Makoto Fukushima1 | Arihiro Kohara2 |Yukio Nakamura1

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Non-viral expression of chimeric antigen receptors with multiplex gene editing in primary T cells

EFFICIENT COST-EFFECTIVE MANUFACTURE OF A NON-VIRAL TRANSPOSON BASED NOVEL BAFF-CART FOR TREATMENT OF B-CELL CANCERS

The PP2A-Integrator-CDK9 axis fine-tunes transcription and can be targeted therapeutically in cancer

Gravity-perfused airway-on-a-chip optimized for quantitative BSL-3 studies of SARS-CoV-2 infection: barrier permeability, cytokine production, immunohistochemistry, and viral load assays

Integration of ?-deficient CARs into the CD3-zeta gene conveys potent cytotoxicity in T and NK cells

Breast cancer-on-chip for patient-specific efficacy and safety testing of CAR-T cells

Robust differentiation of NK cells from MSLN.CAR-IL-15–engineered human iPSCs with enhanced antitumor efficacy against solid tumors

IRF8 regulates efficacy of therapeutic anti-CD20 monoclonal antibodies

Immunocompetent Intestine-on-Chip Model for Analyzing Gut Mucosal Immune Responses

THP-1 reference data: Proposal of an in vitro branched evolution model for cancer cell lines