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Genome-wide CRISPR Screening to Identify Drivers of TGF-ß-Induced Liver Fibrosis in Human Hepatic Stellate Cells

Liver fibrosis progression in chronic liver disease leads to cirrhosis, liver failure, or hepatocellular carcinoma and often ends in liver transplantation. Even with an increased understanding of liver fibrogenesis and many attempts to generate...

Authors: Shan Yu,*† Matthew Ericson,† Andrea Fanjul,† Derek M. Erion,‡ Maria Paraskevopoulou,‡ Erin N....

SST Peer Reviewed

A microvascularized in vitro liver model for disease modeling and drug discovery

Drug discovery for complex liver diseases faces alarming attrition rates. The lack of non-clinical models that recapitulate key aspects of liver (patho)-physiology is likely contributing to the inefficiency of developing effective...

Authors: Flavio Bonanini, Roelof Dinkelberg, Manuel Caro Torregrosa , Nienke Kortekaas , Tessa M. S.

Point-of-use, automated fabrication of a 3D human liver model supplemented with human adipose microvessels

Advanced in vitro tissue models better reflect healthy and disease tissue conditions in the body. However, complex tissue models are often manufactured using custom solutions and can be challenging to manufacture to scale. Here, we describe the...

Authors: Sarah M Moss , Jillian Schilp, Maya Yaakov , Madison Cook , Erik Schuschke , Brandon Hanke , Hannah...

Naturally occurring T cell mutations enhance engineered T cell therapies

Adoptive T cell therapies have produced exceptional responses in a subset of patients with cancer. However, therapeutic efficacy can be hindered by poor T cell persistence and function1. In human T cell cancers, evolution of the disease positively...

Authors: Garcia J, Daniels J, Lee Y, Zhu I, Cheng K, Liu Q, Goodman D, Burnett C, Law C, Thienpont C, Alavi...

SST Peer Reviewed

Liver organoids: updates on generation strategies and biomedical applications

The liver is the most important metabolic organ in the body. While mouse models and cell lines have further deepened our understanding of liver biology and related diseases, they are flawed in replicating key aspects of human liver tissue,...

Authors: Sen Liu , Chuanliang Cheng , Liuyang Zhu , Tianyu Zhao , Ze Wang , Xiulin Yi , Fengying...

Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells

Directed differentiation of human induced pluripotent stem cells (iPSCs) into hepatocytes could provide an unlimited source of liver cells, and therefore holds great promise for regenerative medicine, disease modeling, drug screening and toxicology...

Authors: Rong Li , Yang Zhao , Jeffrey J Yourick , Robert L Sprando , Xiugong Gao

Generation of Complex Syngeneic Liver Organoids from Induced Pluripotent Stem Cells to Model Human Liver Pathophysiology

The study of human liver pathophysiology has been hampered for decades by the lack of easily accessible, robust, and representative in vitro models. The discovery of induced pluripotent stem cells (iPSCs)—which can be generated from...

Authors: Claudia Raggi , Silvia Selleri , Marie-Agnes M'Callum , Massimiliano Paganelli

Liver organoids: from basic research to therapeutic applications

Organoid cultures have emerged as an alternative in vitro system to recapitulate tissues in a dish. While mouse models and cell lines have furthered our understanding of liver biology and associated diseases, they suffer in replicating key aspects of...

Authors: Nicole Prior , Patricia Inacio , Meritxell Huch

Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media

Advances in organoid technology have broadened the number of target diseases and conditions in which human induced pluripotent stem cell (iPSC)-based regenerative medicine can be applied; however, mass production of organoids and the development of...

Authors: Keisuke Sekine , Shimpei Ogawa, Syusaku Tsuzuki, Tatsuya Kobayashi , Kazuki Ikeda, Noriko...

Molecular and Physiological Effects of Browning Agents on White Adipocytes from Bone Marrow Mesenchymal Stromal Cells

Two different types of adipose depots can be observed in mammals: white adipose tissue (WAT) and brown adipose tissue (BAT). The primary role of WAT is to deposit surplus energy in the form of triglycerides, along with many metabolic and hormonal...

Authors: Girolamo Di Maio, Nicola Alessio, Gianfranco Peluso, Silverio Perrotta, Marcellino Monda, Giovanni...

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