Search Results

Protocol for genomic editing in human resting primary NK cells and NK-92 cells via CRISPR-Cas9 ribonucleoproteins

Here, we present a protocol to perform CRISPR-Cas9 genome editing in human resting primary natural killer (NK) and NK-92 cells. We describe steps for guide RNA selection, ribonucleoprotein (RNP) complex formation, delivery via Nucleofection, and...

Authors: Verhezen T, Lau HW, Van Audenaerde J, Wouters A, Smits E, De Waele J

SST Peer Reviewed

Advanced three-dimensional in vitro liver models to study the activity of anticancer drugs

The liver is one of the most important organs in the human body. It performs many important functions, including being responsible for the metabolism of most drugs, which is often associated with its drug-induced damage. Currently, there are no ideal...

Authors: Agnieszka Zuchowska , Sonia Frojdenfal , Maciej Trzaskowski , Elzbieta Jastrzebska

A BAFF ligand-based CAR-T cell targeting three receptors and multiple B cell cancers

B cell-activating factor (BAFF) binds the three receptors BAFF-R, BCMA, and TACI, predominantly expressed on mature B cells. Almost all B cell cancers are reported to express at least one of these receptors. Here we develop a BAFF ligand-based...

Authors: Derek P Wong , Nand K Roy , Keman Zhang , Anusha Anukanth, Abhishek Asthana , Nicole J...

GMP-compliant iPS cell lines show widespread plasticity in a new set of differentiation workflows for cell replacement and cancer immunotherapy

Cell therapeutic applications based on induced pluripotent stem cells (iPSCs) appear highly promising and challenging at the same time. Good manufacturing practice (GMP) regulations impose necessary yet demanding requirements for quality and...

Authors: Terheyden-Keighley D, Hühne M, Berger T, Hiller B, Martins S, Gamerschlag A, Sabour D, Meffert A,...

SST Peer Reviewed

Generation of Tetrafluoroethylene-Propylene Elastomer-Based Microfluidic Devices for Drug Toxicity and Metabolism Studies

Polydimethylsiloxane (PDMS) is widely used to fabricate microfluidic organs-on-chips. Using these devices (PDMS-based devices), the mechanical microenvironment of living tissues, such as pulmonary respiration and intestinal peristalsis, can be...

Authors: Emi Sano, Sayaka Deguchi , Naoki Matsuoka , Masahiro Tsuda, Mengyang Wang , Kaori Kosugi Chihiro...

AutoBioTech-A Versatile Biofoundry for Automated Strain Engineering

The inevitable transition from petrochemical production processes to renewable alternatives has sparked the emergence of biofoundries in recent years. Manual engineering of microbes will not be sufficient to meet the ever-increasing demand for novel...

Authors: Tobias Michael Rosch, Julia Tenhaef, Tim Stoltmann, Till Redeker, Dominic Kösters, Niels...

SST Peer Reviewed

In vitro characterization of 3D culture-based differentiation of human liver stem cells

Introduction: The lack of functional hepatocytes poses a significant challenge for drug safety testing and therapeutic applications due to the inability of mature hepatocytes to expand and their tendency to lose functionality in vitro....

Authors: Marta Tapparo , Gabriele Saccu , Chiara Pasquino , Valentina Fonsato , Claudio Medana , Valentina...

Establishment of Humanized Mice from Peripheral Blood Mononuclear Cells or Cord Blood CD34+ Hematopoietic Stem Cells for Immune-Oncology Studies Evaluating New Therapeutic Agents

The clinical success of immune checkpoint modulators and the development of next-generation immune-oncology (IO) agents underscore the need for robust preclinical models to evaluate novel IO therapeutics. Human immune system (HIS) mouse models enable...

Authors: Bhavna Verma , Amy Wesa

SST Peer Reviewed

Modeling the Effects of Protracted Cosmic Radiation in a Human Organ-on-Chip Platform

Galactic cosmic radiation (GCR) is one of the most serious risks posed to astronauts during missions to the Moon and Mars. Experimental models capable of recapitulating human physiology are critical to understanding the effects of radiation on human...

Authors: Daniel Naveed Tavakol , Trevor R Nash , Youngbin Kim Pamela L Graney , Martin Liberman , Sharon...

Long-term HBV infection of engineered cultures of induced pluripotent stem cell-derived hepatocytes

Background: HBV infects ~257 million people and can cause hepatocellular carcinoma. Since current drugs are not curative, novel therapies are needed. HBV infects chimpanzee and human livers. However, chimpanzee studies are severely restricted...

Authors: Yang Yuan , Vedant V Bodke , Christine Lin , Shang Gao , Jalees Rehman , Jisu Li , Salman R...

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Protocol for genomic editing in human resting primary NK cells and NK-92 cells via CRISPR-Cas9 ribonucleoproteins

Advanced three-dimensional in vitro liver models to study the activity of anticancer drugs

A BAFF ligand-based CAR-T cell targeting three receptors and multiple B cell cancers

GMP-compliant iPS cell lines show widespread plasticity in a new set of differentiation workflows for cell replacement and cancer immunotherapy

Generation of Tetrafluoroethylene-Propylene Elastomer-Based Microfluidic Devices for Drug Toxicity and Metabolism Studies

AutoBioTech-A Versatile Biofoundry for Automated Strain Engineering

In vitro characterization of 3D culture-based differentiation of human liver stem cells

Establishment of Humanized Mice from Peripheral Blood Mononuclear Cells or Cord Blood CD34+ Hematopoietic Stem Cells for Immune-Oncology Studies Evaluating New Therapeutic Agents

Modeling the Effects of Protracted Cosmic Radiation in a Human Organ-on-Chip Platform

Long-term HBV infection of engineered cultures of induced pluripotent stem cell-derived hepatocytes