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Efficient hepatocyte differentiation of primary human hepatocyte-derived organoids using three dimensional nanofibers (HYDROX) and their possible application in hepatotoxicity research

Human liver organoids are in vitro three dimensionally (3D) cultured cells that have a bipotent stem cell phenotype. Translational research of human liver organoids for drug discovery has been limited by the challenge of their low hepatic function...

Authors: Yanran Tong, Yukiko Ueyama-Toba, Jumpei Yokota, Hayato Matsui, Masaki Kanai & Hiroyuki Mizuguchi

An evolved AAV variant enables efficient genetic engineering of murine T cells

Precise targeting of large transgenes to T cells using homology-directed repair has been transformative for adoptive cell therapies and T cell biology. Delivery of DNA templates via adeno-associated virus (AAV) has greatly improved knockin...

Authors: Nyberg WA, Ark J, To A, Clouden S, Reeder G, Muldoon JJ, Chung JY, Xie WH, Allain V, Steinhart Z,...

SST Peer Reviewed

Efficient hepatocyte differentiation of primary human hepatocyte-derived organoids using three dimensional nanofibers (HYDROX) and their possible application in hepatotoxicity research

Human liver organoids are in vitro three dimensionally (3D) cultured cells that have a bipotent stem cell phenotype. Translational research of human liver organoids for drug discovery has been limited by the challenge of their low hepatic function...

Authors: Yanran Tong, Yukiko Ueyama-Toba, Jumpei Yokota, Hayato Matsui, Masaki Kanai & Hiroyuki Mizuguchi

Transfection of choanoflagellates illuminates their cell biology and the ancestry of animal septins

As the closest living relatives of animals, choanoflagellates offer unique insights into animal origins and core mechanisms underlying animal cell biology. However, unlike traditional model organisms, such as yeast, flies, and worms,...

Authors: David S Booth , Heather Szmidt-Middleton , Nicole King

Naïve Primary Mouse CD8+ T Cells Retain In Vivo Immune Responsiveness After Electroporation-Based CRISPR/Cas9 Genetic Engineering

CRISPR/Cas9 technology has revolutionized genetic engineering of primary cells. Although its use is gaining momentum in studies on CD8+ T cell biology, it remains elusive to what extent CRISPR/Cas9 affects in vivo function of...

Authors: Pfenninger P, Yerly L, Abe J

SST Peer Reviewed

Genome editing enables reverse genetics of multicellular development in the choanoflagellate Salpingoeca rosetta

In a previous study, we established a forward genetic screen to identify genes required for multicellular development in the choanoflagellate, Salpingoeca rosetta (Levin et al., 2014). Yet, the paucity of reverse genetic tools for choanoflagellates...

Authors: David S Booth , Nicole King

The Inhibitory Receptor Siglec-8 Interacts With FceRI and Globally Inhibits Intracellular Signaling in Primary Mast Cells Upon Activation

Immunomodulation of mast cell (MC) activity is warranted in allergic and inflammatory diseases where MCs have a central role in pathogenesis. Targeting Siglec-8, an inhibitory receptor on MCs and eosinophils, has shown promising activity in...

Authors: Korver W, Wong A, Gebremeskel S, Negri GL, Schanin J, Chang K, Leung J, Benet Z, Luu T, Brock EC,...

STING mediates immune responses in the closest living relatives of animals

Animals have evolved unique repertoires of innate immune genes and pathways that provide their first line of defense against pathogens. To reconstruct the ancestry of animal innate immunity, we have developed the choanoflagellate Monosiga...

Authors: Arielle Woznica , Ashwani Kumar , Carolyn R Sturge , Chao Xing , Nicole King , Julie K Pfeiffer

Engineering tandem CD33xCD146 CAR CIK (cytokine-induced killer) cells to target the acute myeloid leukemia niche

In acute myeloid leukemia (AML), malignant stem cells hijack the normal bone marrow niche where they are largely protected from the current therapeutic approaches. Thus, eradicating these progenitors is the ultimate challenge in the treatment of this...

Authors: Gaia Alberti, Corinne Arsuffi, Alice Pievani, Domenico Salerno, Francesco Mantegazza, Francesco...

Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair

CRISPR-Cas proteins are RNA-guided nucleases used to introduce double-stranded breaks (DSBs) at targeted genomic loci. DSBs are repaired by endogenous cellular pathways such as non-homologous end joining (NHEJ) and homology-directed repair (HDR)....

Authors: Schubert MS, Thommandru B, Woodley J, Turk R, Yan S, Kurgan G, McNeill MS, Rettig GR.

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Efficient hepatocyte differentiation of primary human hepatocyte-derived organoids using three dimensional nanofibers (HYDROX) and their possible application in hepatotoxicity research

An evolved AAV variant enables efficient genetic engineering of murine T cells

Efficient hepatocyte differentiation of primary human hepatocyte-derived organoids using three dimensional nanofibers (HYDROX) and their possible application in hepatotoxicity research

Transfection of choanoflagellates illuminates their cell biology and the ancestry of animal septins

Naïve Primary Mouse CD8+ T Cells Retain In Vivo Immune Responsiveness After Electroporation-Based CRISPR/Cas9 Genetic Engineering

Genome editing enables reverse genetics of multicellular development in the choanoflagellate Salpingoeca rosetta

The Inhibitory Receptor Siglec-8 Interacts With FceRI and Globally Inhibits Intracellular Signaling in Primary Mast Cells Upon Activation

STING mediates immune responses in the closest living relatives of animals

Engineering tandem CD33xCD146 CAR CIK (cytokine-induced killer) cells to target the acute myeloid leukemia niche

Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair