Search Results

Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism

Therapeutic prime editing of hematopoietic stem and progenitor cells (HSPCs) holds great potential to remedy blood disorders. Quiescent cells have low nucleotide levels and resist retroviral infection, and it is possible that nucleotide metabolism...

Authors: Sébastien Levesque , Andrea Cosentino, Archana Verma , Pietro Genovese , Daniel E Bauer

THP-1 reference data: Proposal of an in vitro branched evolution model for cancer cell lines

THP-1 is a representative leukemia cell line and is registered with four different numbers in JCRB and RIKEN BRC cell banks. However, differences between these four lines remain unclear. In our study, these four THP-1 cell lines, JCRB0112, JCRB0112.1...

Authors: Fumio Kasai1,2 | Noriko Hirayama2 | Makoto Fukushima1 | Arihiro Kohara2 |Yukio Nakamura1

Prediction of tumor-reactive T cell receptors from scRNA-seq data for personalized T cell therapy

The identification of patient-derived, tumor-reactive T cell receptors (TCRs) as a basis for personalized transgenic T cell therapies remains a time- and cost-intensive endeavor. Current approaches to identify tumor-reactive TCRs analyze tumor...

Authors: C L Tan , K Lindner, T Boschert , Z Meng , A Rodriguez Ehrenfried , A De Roia , G Haltenhof , A...

Variable characteristics overlooked in human K-562 leukemia cell lines with a common signature

K-562 is a well-known in vitro cellular model that represents human leukemia cell lines. Although the K-562 cells have been extensively characterized, there are inconsistencies in the data across publications, showing the presence of multiple K-562...

Authors: Fumio Kasai *, Kumiko Mizukoshi & Yukio Nakamura

Liver three-dimensional cellular models for high-throughput chemical testing

Drug-induced hepatotoxicity is a leading cause of drug withdrawal from the market. High-throughput screening utilizing in vitro liver models is critical for early-stage liver toxicity testing. Traditionally, monolayer human hepatocytes or...

Authors: Shu Yang , Masato Ooka , Ryan Jared Margolis , Menghang Xia

Breast cancer-on-chip for patient-specific efficacy and safety testing of CAR-T cells

Physiologically relevant human models that recapitulate the challenges of solid tumors and the tumor microenvironment (TME) are highly desired in the chimeric antigen receptor (CAR)-T cell field. We developed a breast cancer-on-chip model with an...

Authors: Tengku Ibrahim Maulana , Claudia Teufel, Madalena Cipriano , Julia Roosz , Lisa...

Immunocompetent Intestine-on-Chip Model for Analyzing Gut Mucosal Immune Responses

An advanced intestine-on-chip model recreating epithelial 3D organotypic villus-like and crypt-like structures has been developed. The immunocompetent model includes Human Umbilical Vein Endothelial Cells (HUVEC), Caco-2 intestinal epithelial cells,...

Authors: Adrian Feile , Valentin D Wegner , Martin Raasch , Alexander S Mosig

CRISPR Screen Reveals BRD2/4 Molecular Glue-like Degrader via Recruitment of DCAF16

Molecular glues (MGs) are monovalent small molecules that induce an interaction between proteins (native or non-native partners) by altering the protein- protein interaction (PPI) interface toward a higher-affinity state. Enhancing the PPI between a...

Authors: Andrea G. Shergalis, Violeta L. Marin, David Y. Rhee, Sameera Senaweera, Rebecca L. McCloud,Judith...

SST Peer Reviewed

Degradation of IKZF1 prevents epigenetic progression of T cell exhaustion in a novel antigen-specific assay

In cancer, chronic antigen stimulation drives effector T cells to exhaustion, limiting the efficacy of T cell therapies. Recent studies have demonstrated that epigenetic rewiring governs the transition of T cells from effector to exhausted states and...

Authors: Tristan Tay, Gayathri Bommakanti, Elizabeth Jaensch, Aparna Gorthi, Iswarya Karapa Reddy, Yan...

SST Peer Reviewed

Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms

The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a grave threat to public health and the global economy. SARS-CoV-2 is closely related to the more lethal but less transmissible coronaviruses SARS-CoV-1...

Authors: David E. Gordon*, Joseph Hiatt*, Mehdi Bouhaddou*, Veronica V. Rezelj*, Svenja Ulferts*, Hannes...

SST Peer Reviewed

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Research Area

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Topic

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Characteristic

Format

Origin

Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism

THP-1 reference data: Proposal of an in vitro branched evolution model for cancer cell lines

Prediction of tumor-reactive T cell receptors from scRNA-seq data for personalized T cell therapy

Variable characteristics overlooked in human K-562 leukemia cell lines with a common signature

Liver three-dimensional cellular models for high-throughput chemical testing

Breast cancer-on-chip for patient-specific efficacy and safety testing of CAR-T cells

Immunocompetent Intestine-on-Chip Model for Analyzing Gut Mucosal Immune Responses

CRISPR Screen Reveals BRD2/4 Molecular Glue-like Degrader via Recruitment of DCAF16

Degradation of IKZF1 prevents epigenetic progression of T cell exhaustion in a novel antigen-specific assay

Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms