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Automation, Monitoring, and Standardization of Cell Product Manufacturing

Although regenerative medicine products are at the forefront of scientific research, technological innovation, and clinical translation, their reproducibility and large-scale production are compromised by automation, monitoring, and standardization...

Authors: Meletios-Nikolaos Doulgkeroglou 1 2, Alessia Di Nubila 1 2, Bastian Niessing 3, Niels...

SST Peer Reviewed

A reference human induced pluripotent stem cell line for large-scale collaborative studies

Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research...

Authors: Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, Oguro H, Kanaujiya J, Zou J,...

SST Peer Reviewed

Long-term HBV infection of engineered cultures of induced pluripotent stem cell-derived hepatocytes

Background: HBV infects ~257 million people and can cause hepatocellular carcinoma. Since current drugs are not curative, novel therapies are needed. HBV infects chimpanzee and human livers. However, chimpanzee studies are severely restricted...

Authors: Yang Yuan , Vedant V Bodke , Christine Lin , Shang Gao , Jalees Rehman , Jisu Li , Salman R...

The NK cell checkpoint NKG2A maintains expansion capacity of human NK cells

Human natural killer (NK) cells are cytotoxic effector cells that are increasingly harnessed in cancer immunotherapy. NKG2A/CD94 is an inhibitory receptor on NK cells that has established regulatory functions in the direct interaction with target...

Authors: Kaulfuss M, Mietz J, Fabri A, Vom Berg J, Münz C, Chijioke O

SST Peer Reviewed

A microvascularized in vitro liver model for disease modeling and drug discovery

Drug discovery for complex liver diseases faces alarming attrition rates. The lack of non-clinical models that recapitulate key aspects of liver (patho)-physiology is likely contributing to the inefficiency of developing effective...

Authors: Flavio Bonanini, Roelof Dinkelberg, Manuel Caro Torregrosa , Nienke Kortekaas , Tessa M. S.

Protocol for the design, conduct, and evaluation of prime editing in human pluripotent stem cells

Prime editing introduces single-nucleotide polymorphism changes, small dele tions, or insertions at a specific genome site without double-stranded DNA breaks or the need for the donor template. Here, we present a protocol to design, conduct, and...

Authors: Wu Y, Sidharta M, Zhong A, Persily B, Li M, Zhou T

SST Peer Reviewed

An evolved AAV variant enables efficient genetic engineering of murine T cells

Precise targeting of large transgenes to T cells using homology-directed repair has been transformative for adoptive cell therapies and T cell biology. Delivery of DNA templates via adeno-associated virus (AAV) has greatly improved knockin...

Authors: Nyberg WA, Ark J, To A, Clouden S, Reeder G, Muldoon JJ, Chung JY, Xie WH, Allain V, Steinhart Z,...

SST Peer Reviewed

Naturally occurring T cell mutations enhance engineered T cell therapies

Adoptive T cell therapies have produced exceptional responses in a subset of patients with cancer. However, therapeutic efficacy can be hindered by poor T cell persistence and function1. In human T cell cancers, evolution of the disease positively...

Authors: Garcia J, Daniels J, Lee Y, Zhu I, Cheng K, Liu Q, Goodman D, Burnett C, Law C, Thienpont C, Alavi...

SST Peer Reviewed

Naïve Primary Mouse CD8+ T Cells Retain In Vivo Immune Responsiveness After Electroporation-Based CRISPR/Cas9 Genetic Engineering

CRISPR/Cas9 technology has revolutionized genetic engineering of primary cells. Although its use is gaining momentum in studies on CD8+ T cell biology, it remains elusive to what extent CRISPR/Cas9 affects in vivo function of...

Authors: Pfenninger P, Yerly L, Abe J

SST Peer Reviewed

Molecular and Physiological Effects of Browning Agents on White Adipocytes from Bone Marrow Mesenchymal Stromal Cells

Two different types of adipose depots can be observed in mammals: white adipose tissue (WAT) and brown adipose tissue (BAT). The primary role of WAT is to deposit surplus energy in the form of triglycerides, along with many metabolic and hormonal...

Authors: Girolamo Di Maio, Nicola Alessio, Gianfranco Peluso, Silverio Perrotta, Marcellino Monda, Giovanni...

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Topic

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Automation, Monitoring, and Standardization of Cell Product Manufacturing

A reference human induced pluripotent stem cell line for large-scale collaborative studies

Long-term HBV infection of engineered cultures of induced pluripotent stem cell-derived hepatocytes

The NK cell checkpoint NKG2A maintains expansion capacity of human NK cells

A microvascularized in vitro liver model for disease modeling and drug discovery

Protocol for the design, conduct, and evaluation of prime editing in human pluripotent stem cells

An evolved AAV variant enables efficient genetic engineering of murine T cells

Naturally occurring T cell mutations enhance engineered T cell therapies

Naïve Primary Mouse CD8+ T Cells Retain In Vivo Immune Responsiveness After Electroporation-Based CRISPR/Cas9 Genetic Engineering

Molecular and Physiological Effects of Browning Agents on White Adipocytes from Bone Marrow Mesenchymal Stromal Cells