Search Results

Evaluation of the Biological Activity of a GH Mutant (R77C) and its Impact on GH Responsiveness and Stature

Context and Objective: A single missense mutation in the GH-1 gene converting codon 77 from arginine (R) to cysteine (C) yields a mutant GH-R77C peptide, which was described as natural growth hormone antagonist. Design, Setting, Patients:...

Authors: Petkovic V, Besson A, Thevis M, Lochmatter D, Eblé A, Flück CE, Mullis PE

Bcl-2 cleavages at two adjacent sites by different caspases promote cisplatin-induced apoptosis

The protein encoded by bcl-2 proto-oncogene plays an important role in the mitochondria-mediated apoptotic pathway. Although the general role of Bcl-2 is anti-apoptotic, previous work showed that Bcl-2 fragments cleaved by caspases could promote...

Authors: Zhu J, Yang Y, Wu J

Investigation of the dimerization of proteins from the epidermal growth factor receptor family by single wavelength fluorescence cross-correlation spectroscopy

Single wavelength fluorescence cross-correlation spectroscopy (SW-FCCS), introduced to study biomolecular interactions, has recently been reported to monitor enzyme activity by using a newly developed fluorescent protein variant together with cyan...

Authors: Liu P, Sudhaharan T, Koh RM, Hwang LC, Ahmed S, Maruyama IN, Wohland T

The localization and activity of sphingosine kinase 1 are coordinately-regulated with actin cytoskeletal dynamics in macrophages

The physiologic and pathologic functions of sphingosine kinase (SK)1 require translocation to specific membrane compartments. We tested the hypothesis that interactions with actin filaments regulate the localization of SK1 to membrane surfaces;...

Authors: Kusner DJ, Thompson CR, Melrose NA, Pitson SM, Obeid LM, Iyer SS

SST Peer Reviewed

Sequential Activation of Poly(ADP-Ribose) Polymerase 1, Calpains, and Bax Is Essential in Apoptosis-Inducing Factor-Mediated Programmed Necrosis

Alkylating DNA damage induces a necrotic type of programmed cell death through the poly(ADP-ribose) polymerases (PARP) and apoptosis-inducing factor (AIF). Following PARP activation, AIF is released from mitochondria and translocates to the nucleus,...

Authors: Moubarak RS, Yuste VJ, Artus C, Bouharrour A, Greer PA, Menissier-de Murcia J, Susin SA

Vaccination with NY-ESO-1 protein and CpG in Montanide induces integrated antibody/Th1 responses and CD8 T cells through cross-priming

The use of recombinant tumor antigen proteins is a realistic approach for the development of generic cancer vaccines, but the potential of this type of vaccines to induce specific CD8(+) T cell responses, through in vivo cross-priming, has remained...

Authors: Valmori D, Souleimanian NE, Tosello V, Bhardwaj N, Adams S, O'neill D, Pavlick A, Escalon JB, Cruz...

In vitro models of TGF-beta-induced fibrosis suitable for high-throughput screening of antifibrotic agents

Progressive fibrosis is a cause of progressive organ dysfunction. Lack of quantitative in vitro models of fibrosis accounts, at least partially, for the slow progress in developing effective anti-fibrotic drugs. Here we report two complementary in...

Authors: Xu Q, Norman JT, Shrivastav S, Lucio-Cazana J, Kopp JB

SST Peer Reviewed

NRAS mutation causes a human autoimmune lymphoproliferative syndrome

The p21 RAS subfamily of small GTPases, including KRAS, HRAS, and NRAS, regulates cell proliferation, cytoskeletal organization, and other signaling networks, and is the most frequent target of activating mutations in cancer. Activating germline...

Authors: Oliveira JB, Bidère N, Niemela JE, Zheng L, Sakai K, Nix CP, Danner RL, Barb J, Munson PJ, Puck JM,...

Involvement of integrin-induced activation of protein kinase C in the formation of adherens junctions

In epithelial cells, tight junctions (TJs) and adherens junctions (AJs) form junctional complexes. At AJs, cadherins and nectins are the major cell-cell adhesion molecules. Nectins first form cell-cell adhesions and then recruit cadherins to the...

Authors: Ozaki M, Ogita H, Takai Y

Vascular endothelial growth factor can signal through platelet-derived growth factor receptors

Vascular endothelial growth factor (VEGF-A) is a crucial stimulator of vascular cell migration and proliferation. Using bone marrow-derived human adult mesenchymal stem cells (MSCs) that did not express VEGF receptors, we provide evidence that VEGF-A...

Authors: Ball SG, Shuttleworth CA, Kielty CM

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