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Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms

The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a grave threat to public health and the global economy. SARS-CoV-2 is closely related to the more lethal but less transmissible coronaviruses SARS-CoV-1...

Authors: David E. Gordon*, Joseph Hiatt*, Mehdi Bouhaddou*, Veronica V. Rezelj*, Svenja Ulferts*, Hannes...

SST Peer Reviewed

Comparison of two lab-scaleprotocols for enhanced mRNA-based CAR-T cell generationand functionality

Process development for transferring lab-scale research workflows to automated manufacturing procedures is critical for chimeric antigen receptor (CAR)-T cell therapies. Therefore, the key factor for cell viability, expansion, modification, and...

Authors: von Auw N, Serfling R, Kitte R, Hilger N, Zhang C, Gebhardt C, Duenkel A, Franz P, Koehl U, Fricke...

SST Peer Reviewed

Protocol for genomic editing in human resting primary NK cells and NK-92 cells via CRISPR-Cas9 ribonucleoproteins

Here, we present a protocol to perform CRISPR-Cas9 genome editing in human resting primary natural killer (NK) and NK-92 cells. We describe steps for guide RNA selection, ribonucleoprotein (RNP) complex formation, delivery via Nucleofection, and...

Authors: Verhezen T, Lau HW, Van Audenaerde J, Wouters A, Smits E, De Waele J

SST Peer Reviewed

A novel Cas9 fusion protein promotes targeted genome editing with reduced mutational burden in primary human cells

Precise genome editing requires the resolution of nuclease-induced DNA double strand breaks (DSBs) via the homology-directed repair (HDR) pathway. In mammals, this is typically outcompeted by non-homologous end-joining (NHEJ) that can generate...

Authors: Carusillo A, Haider S, Schäfer R, Rhiel M, Türk D, Chmielewski KO, Klermund J, Mosti L, Andrieux G,...

SST Peer Reviewed

Advanced three-dimensional in vitro liver models to study the activity of anticancer drugs

The liver is one of the most important organs in the human body. It performs many important functions, including being responsible for the metabolism of most drugs, which is often associated with its drug-induced damage. Currently, there are no ideal...

Authors: Agnieszka Zuchowska , Sonia Frojdenfal , Maciej Trzaskowski , Elzbieta Jastrzebska

Automated production of gene-modified chimeric antigen receptor T cells using the Cocoon Platform

Autologous cell-based therapeutics have gained increasing attention in recent years because of their efficacy at treating diseases with limited therapeutic options. Chimeric antigen receptor (CAR) T-cell therapy has demonstrated clinical success in...

Authors: Trainor N, Purpura KA, Middleton K, Fargo K, Hails L, Vicentini-Hogan M, McRobie C, Daniels R,...

SST Peer Reviewed

GMP-compliant iPS cell lines show widespread plasticity in a new set of differentiation workflows for cell replacement and cancer immunotherapy

Cell therapeutic applications based on induced pluripotent stem cells (iPSCs) appear highly promising and challenging at the same time. Good manufacturing practice (GMP) regulations impose necessary yet demanding requirements for quality and...

Authors: Terheyden-Keighley D, Hühne M, Berger T, Hiller B, Martins S, Gamerschlag A, Sabour D, Meffert A,...

SST Peer Reviewed

Modulating mutational outcomes and improving precise gene editing at CRISPR-Cas9-induced breaks by chemical inhibition of end-joining pathways

Gene editing through repair of CRISPR-Cas9-induced chromosomal breaks offers a means to correct a wide range of genetic defects. Directing repair to produce desirable outcomes by modulating DNA repair pathways holds considerable promise to increase...

Authors: Schimmel J, Muñoz-Subirana N, Kool H, van Schendel R, van der Vlies S, Kamp JA, de Vrij FMS, Kushner...

SST Peer Reviewed

AutoBioTech-A Versatile Biofoundry for Automated Strain Engineering

The inevitable transition from petrochemical production processes to renewable alternatives has sparked the emergence of biofoundries in recent years. Manual engineering of microbes will not be sufficient to meet the ever-increasing demand for novel...

Authors: Tobias Michael Rosch, Julia Tenhaef, Tim Stoltmann, Till Redeker, Dominic Kösters, Niels...

SST Peer Reviewed

In vitro characterization of 3D culture-based differentiation of human liver stem cells

Introduction: The lack of functional hepatocytes poses a significant challenge for drug safety testing and therapeutic applications due to the inability of mature hepatocytes to expand and their tendency to lose functionality in vitro....

Authors: Marta Tapparo , Gabriele Saccu , Chiara Pasquino , Valentina Fonsato , Claudio Medana , Valentina...

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Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms

Comparison of two lab-scaleprotocols for enhanced mRNA-based CAR-T cell generationand functionality

Protocol for genomic editing in human resting primary NK cells and NK-92 cells via CRISPR-Cas9 ribonucleoproteins

A novel Cas9 fusion protein promotes targeted genome editing with reduced mutational burden in primary human cells

Advanced three-dimensional in vitro liver models to study the activity of anticancer drugs

Automated production of gene-modified chimeric antigen receptor T cells using the Cocoon Platform

GMP-compliant iPS cell lines show widespread plasticity in a new set of differentiation workflows for cell replacement and cancer immunotherapy

Modulating mutational outcomes and improving precise gene editing at CRISPR-Cas9-induced breaks by chemical inhibition of end-joining pathways

AutoBioTech-A Versatile Biofoundry for Automated Strain Engineering

In vitro characterization of 3D culture-based differentiation of human liver stem cells