Search Results

Modeling of Blood–Brain Barrier (BBB) Dysfunction and Immune Cell Migration Using Human BBB-on-a-Chip for Drug Discovery Research

Blood–brain barrier (BBB) dysfunction is a key feature in neuroimmunological and neurodegenerative diseases. In this study, we developed a microfluidic human BBB-on-a-chip to model barrier dysfunction and immune cell migration using immortalized TY10...

Authors: Masato Ohbuchi , Mayu Shibuta , Kazuhiro Tetsuka , Haruna Sasaki-Iwaoka , Masayo Oishi , Fumitaka...

Liver three-dimensional cellular models for high-throughput chemical testing

Drug-induced hepatotoxicity is a leading cause of drug withdrawal from the market. High-throughput screening utilizing in vitro liver models is critical for early-stage liver toxicity testing. Traditionally, monolayer human hepatocytes or...

Authors: Shu Yang , Masato Ooka , Ryan Jared Margolis , Menghang Xia

p63 controls metabolic activation of hepatic stellate cells and fibrosis via an HER2-ACC1 pathway

The p63 protein has pleiotropic functions and, in the liver, participates in the progression of nonalcoholic fatty liver disease (NAFLD). However, its functions in hepatic stellate cells (HSCs) have not yet been explored. TAp63 is induced in HSCs...

Authors: Marcos F Fondevila , Eva Novoa , Maria J Gonzalez-Rellan , Uxia Fernandez , Violeta Heras , Begoña...

CRISPR Screen Reveals BRD2/4 Molecular Glue-like Degrader via Recruitment of DCAF16

Molecular glues (MGs) are monovalent small molecules that induce an interaction between proteins (native or non-native partners) by altering the protein- protein interaction (PPI) interface toward a higher-affinity state. Enhancing the PPI between a...

Authors: Andrea G. Shergalis, Violeta L. Marin, David Y. Rhee, Sameera Senaweera, Rebecca L. McCloud,Judith...

SST Peer Reviewed

Degradation of IKZF1 prevents epigenetic progression of T cell exhaustion in a novel antigen-specific assay

In cancer, chronic antigen stimulation drives effector T cells to exhaustion, limiting the efficacy of T cell therapies. Recent studies have demonstrated that epigenetic rewiring governs the transition of T cells from effector to exhausted states and...

Authors: Tristan Tay, Gayathri Bommakanti, Elizabeth Jaensch, Aparna Gorthi, Iswarya Karapa Reddy, Yan...

SST Peer Reviewed

Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms

The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a grave threat to public health and the global economy. SARS-CoV-2 is closely related to the more lethal but less transmissible coronaviruses SARS-CoV-1...

Authors: David E. Gordon*, Joseph Hiatt*, Mehdi Bouhaddou*, Veronica V. Rezelj*, Svenja Ulferts*, Hannes...

SST Peer Reviewed

Protocol for genomic editing in human resting primary NK cells and NK-92 cells via CRISPR-Cas9 ribonucleoproteins

Here, we present a protocol to perform CRISPR-Cas9 genome editing in human resting primary natural killer (NK) and NK-92 cells. We describe steps for guide RNA selection, ribonucleoprotein (RNP) complex formation, delivery via Nucleofection, and...

Authors: Verhezen T, Lau HW, Van Audenaerde J, Wouters A, Smits E, De Waele J

SST Peer Reviewed

A microfluidic platform integrating functional vascularized organoids-on-chip

The development of vascular networks in microfluidic chips is crucial for the long-term culture of three-dimensional cell aggregates such as spheroids, organoids, tumoroids, or tissue explants. Despite rapid advancement in microvascular network...

Authors: Clément Quintard, Emily Tubbs , Gustav Jonsson , Jie Jiao , Jun Wang , Nicolas Werschler , Camille...

Advanced three-dimensional in vitro liver models to study the activity of anticancer drugs

The liver is one of the most important organs in the human body. It performs many important functions, including being responsible for the metabolism of most drugs, which is often associated with its drug-induced damage. Currently, there are no ideal...

Authors: Agnieszka Zuchowska , Sonia Frojdenfal , Maciej Trzaskowski , Elzbieta Jastrzebska

GMP-compliant iPS cell lines show widespread plasticity in a new set of differentiation workflows for cell replacement and cancer immunotherapy

Cell therapeutic applications based on induced pluripotent stem cells (iPSCs) appear highly promising and challenging at the same time. Good manufacturing practice (GMP) regulations impose necessary yet demanding requirements for quality and...

Authors: Terheyden-Keighley D, Hühne M, Berger T, Hiller B, Martins S, Gamerschlag A, Sabour D, Meffert A,...

SST Peer Reviewed

Data Type

Category

Research Area

Platform

Topic

Species

Characteristic

Format

Origin

Modeling of Blood–Brain Barrier (BBB) Dysfunction and Immune Cell Migration Using Human BBB-on-a-Chip for Drug Discovery Research

Liver three-dimensional cellular models for high-throughput chemical testing

p63 controls metabolic activation of hepatic stellate cells and fibrosis via an HER2-ACC1 pathway

CRISPR Screen Reveals BRD2/4 Molecular Glue-like Degrader via Recruitment of DCAF16

Degradation of IKZF1 prevents epigenetic progression of T cell exhaustion in a novel antigen-specific assay

Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms

Protocol for genomic editing in human resting primary NK cells and NK-92 cells via CRISPR-Cas9 ribonucleoproteins

A microfluidic platform integrating functional vascularized organoids-on-chip

Advanced three-dimensional in vitro liver models to study the activity of anticancer drugs

GMP-compliant iPS cell lines show widespread plasticity in a new set of differentiation workflows for cell replacement and cancer immunotherapy