Search Results

Integration of ?-deficient CARs into the CD3-zeta gene conveys potent cytotoxicity in T and NK cells

Chimeric antigen receptor (CAR)-redirected immune cells hold significant therapeutic potential for oncology, autoimmune diseases, transplant medicine, and infections. All approved CAR-T therapies rely on personalized manufacturing using...

Authors: Kath J, Franke C, Drosdek V, Du W, Glaser V, Fuster-Garcia C, Stein M, Zittel T, Schulenberg S,...

SST Peer Reviewed

THP-1 reference data: Proposal of an in vitro branched evolution model for cancer cell lines

THP-1 is a representative leukemia cell line and is registered with four different numbers in JCRB and RIKEN BRC cell banks. However, differences between these four lines remain unclear. In our study, these four THP-1 cell lines, JCRB0112, JCRB0112.1...

Authors: Fumio Kasai1,2 | Noriko Hirayama2 | Makoto Fukushima1 | Arihiro Kohara2 |Yukio Nakamura1

Variable characteristics overlooked in human K-562 leukemia cell lines with a common signature

K-562 is a well-known in vitro cellular model that represents human leukemia cell lines. Although the K-562 cells have been extensively characterized, there are inconsistencies in the data across publications, showing the presence of multiple K-562...

Authors: Fumio Kasai *, Kumiko Mizukoshi & Yukio Nakamura

Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers

Here, we present a protocol for isolating human hepatocytes and neural progenitor cells from normal and nonalcoholic steatohepatitis livers. We describe steps for perfusion for scaled-up liver cell isolation and optimization of chemical digestion to...

Authors: Xiao Liu , Kevin Lam , Huayi Zhao , Sadatsugu Sakane , Hyun Young Kim , Alvaro Eguileor , Karin...

Gene knock-outs in human CD34+hematopoietic stem and progenitor cells andin the human immune system of mice

Human CD34+ hematopoietic stem and progenitor cells (HSPCs) are a standard source of cells for clinical HSC transplantations as well as experimental xenotransplantation to generate “humanized mice”. To further extend the range of applications of...

Authors: Daniel A Kuppers, Jonathan Linton, Sergio Ortiz Espinosa, Kelly M McKenna, Anthony Rongvaux, Patrick...

Liver three-dimensional cellular models for high-throughput chemical testing

Drug-induced hepatotoxicity is a leading cause of drug withdrawal from the market. High-throughput screening utilizing in vitro liver models is critical for early-stage liver toxicity testing. Traditionally, monolayer human hepatocytes or...

Authors: Shu Yang , Masato Ooka , Ryan Jared Margolis , Menghang Xia

Mitigation of chromosome loss in clinical CRISPR Cas9-engineered T cells

CRISPR-Cas9 genome editing has enabled advanced T cell therapies, but occasional loss of the targeted chromosome remains a safety concern. To investigate whether Cas9-induced chromosome loss is a universal phenomenon and evaluate its clinical...

Authors: Tsuchida CA, Brandes N, Bueno R, Trinidad M, Mazumder T, Yu B, Hwang B, Chang C, Liu J, Sun Y,...

SST Peer Reviewed

CRISPR Screen Reveals BRD2/4 Molecular Glue-like Degrader via Recruitment of DCAF16

Molecular glues (MGs) are monovalent small molecules that induce an interaction between proteins (native or non-native partners) by altering the protein- protein interaction (PPI) interface toward a higher-affinity state. Enhancing the PPI between a...

Authors: Andrea G. Shergalis, Violeta L. Marin, David Y. Rhee, Sameera Senaweera, Rebecca L. McCloud,Judith...

SST Peer Reviewed

Large-scale manufacturing of base-edited chimeric antigen receptor T cells

Base editing is a revolutionary gene-editing technique enabling the introduction of point mutations into the genome without generating detrimental DNA double-stranded breaks. Base-editing enzymes are commonly delivered in the form...

Authors: Woodruff R, Parekh F, Lamb K, Mekkaoui L, Allen C, Smetanova K, Huang J, Williams A, Toledo GS,...

SST Peer Reviewed

Degradation of IKZF1 prevents epigenetic progression of T cell exhaustion in a novel antigen-specific assay

In cancer, chronic antigen stimulation drives effector T cells to exhaustion, limiting the efficacy of T cell therapies. Recent studies have demonstrated that epigenetic rewiring governs the transition of T cells from effector to exhausted states and...

Authors: Tristan Tay, Gayathri Bommakanti, Elizabeth Jaensch, Aparna Gorthi, Iswarya Karapa Reddy, Yan...

SST Peer Reviewed

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Integration of ?-deficient CARs into the CD3-zeta gene conveys potent cytotoxicity in T and NK cells

THP-1 reference data: Proposal of an in vitro branched evolution model for cancer cell lines

Variable characteristics overlooked in human K-562 leukemia cell lines with a common signature

Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers

Gene knock-outs in human CD34+hematopoietic stem and progenitor cells andin the human immune system of mice

Liver three-dimensional cellular models for high-throughput chemical testing

Mitigation of chromosome loss in clinical CRISPR Cas9-engineered T cells

CRISPR Screen Reveals BRD2/4 Molecular Glue-like Degrader via Recruitment of DCAF16

Large-scale manufacturing of base-edited chimeric antigen receptor T cells

Degradation of IKZF1 prevents epigenetic progression of T cell exhaustion in a novel antigen-specific assay