Search Results

Generation of Tetrafluoroethylene-Propylene Elastomer-Based Microfluidic Devices for Drug Toxicity and Metabolism Studies

Polydimethylsiloxane (PDMS) is widely used to fabricate microfluidic organs-on-chips. Using these devices (PDMS-based devices), the mechanical microenvironment of living tissues, such as pulmonary respiration and intestinal peristalsis, can be...

Authors: Emi Sano, Sayaka Deguchi , Naoki Matsuoka , Masahiro Tsuda, Mengyang Wang , Kaori Kosugi Chihiro...

Highly efficient CRISPR-Cas9-mediated geneknockout in primary human B cells forfunctional genetic studies of Epstein-Barrvirus infection

Gene editing is now routine in all prokaryotic and metazoan cells but has not received much attention in immune cells when the CRISPR-Cas9 technology was introduced in the field of mammalian cell biology less than ten years ago. This versatile...

Authors: Akidil E, Albanese M, Buschle A, Ruhle A, Pich D, Keppler OT, Hammerschmidt W.

Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair

CRISPR-Cas proteins are RNA-guided nucleases used to introduce double-stranded breaks (DSBs) at targeted genomic loci. DSBs are repaired by endogenous cellular pathways such as non-homologous end joining (NHEJ) and homology-directed repair (HDR)....

Authors: Schubert MS, Thommandru B, Woodley J, Turk R, Yan S, Kurgan G, McNeill MS, Rettig GR.

Engineering of potent CAR NK cells using non-viral Sleeping Beauty transposition from minimalistic DNA vectors

Natural Killer (NK) cells have high intrinsic cytotoxic capacity, and clinical trials have demonstrated their safety and efficacy for adoptive cancer therapy. Expression of chimeric antigen receptors (CARs) enhances NK cell target-specificity, with...

Authors: Bexte T, Botezatu L, Miskey C, Gierschek F, Moter A, Wendel P, Reindl LM, Campe J, Villena-Ossa JF,...

SST Peer Reviewed

Addressing the ADME Challenges of Compound Loss in a PDMS-Based Gut-on-Chip Microphysiological System

Microphysiological systems (MPSs) are promising in vitro technologies for physiologically relevant predictions of the human absorption, distribution, metabolism, and excretion (ADME) properties of drug candidates. However, polydimethylsiloxane...

Authors: Patrick Carius , Ferdinand Anton Weinelt , Chris Cantow , Markus Holstein , Aaron M...

Structural surfaceomics reveals an AML-specific conformation of integrin ß2 as a CAR T cellular therapy target

Safely expanding indications for cellular therapies has been challenging given a lack of highly cancer-specific surface markers. Here we explore the hypothesis that tumor cells express cancer-specific surface protein conformations that are invisible...

Authors: Mandal K, Wicaksono G, Yu C, Adams JJ, Hoopmann MR, Temple WC, Izgutdina A, Escobar BP, Gorelik M,...

SST Peer Reviewed

Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism

Therapeutic prime editing of hematopoietic stem and progenitor cells (HSPCs) holds great potential to remedy blood disorders. Quiescent cells have low nucleotide levels and resist retroviral infection, and it is possible that nucleotide metabolism...

Authors: Sébastien Levesque , Andrea Cosentino, Archana Verma , Pietro Genovese , Daniel E Bauer

Modular Pooled Discovery of Synthetic Knockin Sequences to Program Durable Cell Therapies

Chronic stimulation can cause T cell dysfunction and limit efficacy of cellular immunotherapies. CRISPR screens have nominated gene targets for engineered T cells, but improved methods are required to compare large numbers of synthetic knockin...

Authors: F. Blaeschke, Y. Y. Chen, R. Apathy, Z. Li, C. T. Mowery, W.A. Nyberg, A. To, R. Yu, R. Bueno,...

SST Peer Reviewed

Prediction of tumor-reactive T cell receptors from scRNA-seq data for personalized T cell therapy

The identification of patient-derived, tumor-reactive T cell receptors (TCRs) as a basis for personalized transgenic T cell therapies remains a time- and cost-intensive endeavor. Current approaches to identify tumor-reactive TCRs analyze tumor...

Authors: C L Tan , K Lindner, T Boschert , Z Meng , A Rodriguez Ehrenfried , A De Roia , G Haltenhof , A...

Adenine base editing efficiently restores the function of Fanconi anemia hematopoietic stem and progenitor cells

Fanconi Anemia (FA) is a debilitating genetic disorder with a wide range of severe symptoms including bone marrow failure and predisposition to cancer. CRISPR-Cas genome editing manipulates genotypes by harnessing DNA repair and has been proposed as...

Authors: Siegner SM, Ugalde L, Clemens A, Garcia-Garcia L, Bueren JA, Rio P, Karasu ME, Corn JE.

Data Type

Category

Research Area

Platform

Topic

Species

Characteristic

Format

Origin

Generation of Tetrafluoroethylene-Propylene Elastomer-Based Microfluidic Devices for Drug Toxicity and Metabolism Studies

Highly efficient CRISPR-Cas9-mediated geneknockout in primary human B cells forfunctional genetic studies of Epstein-Barrvirus infection

Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair

Engineering of potent CAR NK cells using non-viral Sleeping Beauty transposition from minimalistic DNA vectors

Addressing the ADME Challenges of Compound Loss in a PDMS-Based Gut-on-Chip Microphysiological System

Structural surfaceomics reveals an AML-specific conformation of integrin ß2 as a CAR T cellular therapy target

Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism

Modular Pooled Discovery of Synthetic Knockin Sequences to Program Durable Cell Therapies

Prediction of tumor-reactive T cell receptors from scRNA-seq data for personalized T cell therapy

Adenine base editing efficiently restores the function of Fanconi anemia hematopoietic stem and progenitor cells