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Molecular and Functional Analyses of a Human Parvovirus B19 Infectious Clone Demonstrates Essential Roles for NS1, VP1, and the 11-Kilodalton Protein in Virus Replication and Infectivity

In an attempt to experimentally define the roles of viral proteins encoded by the B19 genome in the viral life cycle, we utilized the B19 infectious clone constructed in our previous study to create two groups of B19 mutant genomes: (i) null mutants,...

Authors: Zhi N, Mills IP, Lu J, Wong S, Filippone C, Brown KE

High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei

This protocol describes a method of genetic transformation for the rodent malaria parasite Plasmodium berghei with a high transfection efficiency of 10(-3)-10(-4). It provides methods for: (i) in vitro cultivation and purification of the schizont...

Authors: Janse CJ, Ramesar J, Waters AP

SST Peer Reviewed

Selection by flow-sorting of genetically transformed, GFP-expressing blood stages of the rodent malaria parasite, Plasmodium berghei

This protocol describes a methodology for the genetic transformation of the rodent malaria parasite Plasmodium berghei and the subsequent selection of transformed parasites expressing green fluorescent protein (GFP) by flow-sorting. It provides...

Authors: Janse CJ, Franke-Fayard B, Waters AP

SST Peer Reviewed

Anti-MCP-1 Gene Therapy Inhibits Vascular Smooth Muscle Cells Proliferation and Attenuates Vein Graft Thickening Both In Vitro and In Vivo

OBJECTIVE: Because late vein graft failure is caused by intimal hyperplasia (IH) and accelerated atherosclerosis, and these processes are thought to be inflammation driven, influx of monocytes is one of the first phenomena seen in IH, we would like...

Authors: Schepers A, Eefting D, Bonta PI, Grimbergen JM, de Vries MR, van Weel V, de Vries CJ, Egashira K,...

A common ankyrin-G-based mechanism retains KCNQ and NaV channels at electrically active domains of the axon

KCNQ (KV7) potassium channels underlie subthreshold M-currents that stabilize the neuronal resting potential and prevent repetitive firing of action potentials. Here, antibodies against four different KCNQ2 and KCNQ3 polypeptide epitopes show these...

Authors: Pan Z, Kao T, Horvath Z, Lemos J, Sul JY, Cranstoun SD, Bennett V, Scherer SS, Cooper EC

MITF Interactions with 14-3-3 Modulate Differentiation of Committed Myeloid Precursors

The microphthalmia-associated transcription factor (MITF) is required for terminal osteoclast differentiation and is a target for signaling pathways engaged by colony stimulating factor (CSF)-1 and receptor-activator of nuclear factor-kappaB ligand...

Authors: Bronisz A, Sharma SM, Hu R, Godlewski J, Tzivion G, Mansky KC, Ostrowski MC

Protein phosphatase 2A is required for mesalazine-dependent inhibition of Wnt/-catenin pathway activity

The rising incidence and poor prognosis of colorectal cancer have aroused substantial interest in novel chemopreventive strategies. Interestingly, treatment of ulcerative colitis with mesalazine, which displays few side effects during long-term...

Authors: Bos CL, Diks SH, Hardwick JC, Walburg KV, Peppelenbosch MP, Richel DJ

CIB1, a ubiquitously-expressed Ca2+-binding protein-ligand of the InsP3 receptor Ca2+-release channel

A family of Ca2+ binding proteins (CaBPs) was shown to bind to the inositol 1,4,5-trisphosphate receptor (InsP3R) Ca2+ release channel and gate it in the absence of InsP3, establishing them as protein ligands (Yang et al. (2002) Identification of a...

Authors: White C, Yang J, Monteiro MJ, Foskett JK

Eph receptors inactivate R-Ras through different mechanisms to achieve cell repulsion

Eph receptor tyrosine kinases regulate the spatial organization of cells within tissues. Central to this function is their ability to modulate cell shape and movement in response to stimulation by the ephrin ligands. The EphB2 receptor was reported...

Authors: Dail M, Richter M, Godement P, Pasquale EB

Reduction in ABCG1 in type 2 diabetic mice increases macrophage foam cell formation

Atherosclerosis development is accelerated several-fold in patients with Type 2 diabetes. In the initial stages of disease, monocytes transmigrate into the subendothelial space and differentiate into foam cells. Scavenger receptors and ATP Binding...

Authors: Mauldin JP, Srinivasan S, Mulya A, Gebre A, Parks JS, Daugherty A, Hedrick CC

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