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Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

The CRISPR/Cas9 system is a robust genome editing technology that works in human cells, animals and plants based on the RNA-programmed DNA cleaving activity of the Cas9 enzyme. Building on previous work (Jinek et al., 2013), we show here that new...

Authors: Lin S, Staahl BT, Alla RK, Doudna JA.

The miR-424(322)/503 cluster orchestrates remodeling of the epithelium in the involuting mammary gland

The mammary gland is a very dynamic organ that undergoes continuous remodeling. The critical regulators of this process are not fully understood. Here we identify the microRNA cluster miR-424(322)/503 as an important regulator of epithelial...

Authors: Llobet-Navas D, Rodríguez-Barrueco R, Castro V, Ugalde AP, Sumazin P, Jacob-Sendler D, Demircan B,...

Heterologous viral promoters incorporated into the human cytomegalovirus genome are silenced during experimental latency.

Human cytomegalovirus (HCMV) lytic phase gene expression is repressed upon entry into myeloid lineage cells where the virus establishes latency. Lytic infection is not initiated because the tegument-delivered transactivator protein pp71 fails to...

Authors: Qin Q, Penkert RR, Kalejta RF

SST Peer Reviewed

In vitro antitumor activity of stellettin B, a triterpene from marine sponge Jaspis stellifera, on human glioblastoma cancer SF295 cells

Stellettin B was isolated from marine sponge Jaspis stellifera. In vitro antitumor activities were investigated on 39 human cancer cell lines. Stellettin B exhibited highly potent inhibition against the growth of a human glioblastoma cell line SF295,...

Authors: Tang SA, Zhou Q, Guo WZ, Qiu Y, Wang R, Jin M, Zhang W, Li K, Yamori T, Dan S, Kong D

Cell-state transitions regulated by SLUG are critical for tissue regeneration and tumor initiation.

Perturbations in stem cell activity and differentiation can lead to developmental defects and cancer. We use an approach involving a quantitative model of cell-state transitions in vitro to gain insights into how SLUG/SNAI2, a key developmental...

Authors: Phillips S1, Prat A2, Sedic M1, Proia T3, Wronski A1, Mazumdar S4, Skibinski A1, Shirley SH5, Perou...

Iron deprivation blocks multilineage haematopoietic differentiation by inhibiting induction of p21(WAF1/CIP1).

Iron is required for the differentiation of HL-60 cells along the monocyte lineage in vitro, reflecting a requirement for iron in the transcriptional induction of the p21(WAF1/CIP1) gene. To determine if the same requirement holds true for...

Authors: Alcantara O, Boldt DH

SST Peer Reviewed

Possible Mechanism of Low Endotoxin Recovery

Authors: M.Tsuchiya

Resveratrol ameliorates TNFa-mediated suppression of erythropoiesis in human CD34(+) cells via modulation of NF-?B signalling.

Overexpression of pro-inflammatory cytokines, including tumour necrosis factor alpha (TNFa), has been implicated in the pathogenesis of anaemia of inflammation. TNFa suppresses erythroid colony formation via both direct and indirect effects on...

Authors: Jeong JY, Silver M, Parnes A, Nikiforow S, Berliner N, Vanasse GJ.

SST Peer Reviewed

SOX4 mediates TGF-ß-induced expression of mesenchymal markers during mammary cell epithelial to mesenchymal transition.

The epithelial to mensenchymal transition program regulates various aspects of embryonic development and tissue homeostasis, but aberrant activation of this pathway in cancer contributes to tumor progression and metastasis. TGF-b potently induces an...

Authors: Vervoort SJ, Lourenço AR, van Boxtel R, Coffer PJ.

Carbon nanotube fibers are compatible with Mammalian cells and neurons.

We demonstrate the biocompatibility of carbon nanotube fibers (CNFs) fabricated from single-wall carbon nanotubes. Produced by a particle-coagulation spinning process, CNFs are "hair-like" conductive microwires, which uniquely combine properties of...

Authors: Dubin RA, Callegari G, Kohn J, Neimark A.

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Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

The miR-424(322)/503 cluster orchestrates remodeling of the epithelium in the involuting mammary gland

Heterologous viral promoters incorporated into the human cytomegalovirus genome are silenced during experimental latency.

In vitro antitumor activity of stellettin B, a triterpene from marine sponge Jaspis stellifera, on human glioblastoma cancer SF295 cells

Cell-state transitions regulated by SLUG are critical for tissue regeneration and tumor initiation.

Iron deprivation blocks multilineage haematopoietic differentiation by inhibiting induction of p21(WAF1/CIP1).

Possible Mechanism of Low Endotoxin Recovery

Resveratrol ameliorates TNFa-mediated suppression of erythropoiesis in human CD34(+) cells via modulation of NF-?B signalling.

SOX4 mediates TGF-ß-induced expression of mesenchymal markers during mammary cell epithelial to mesenchymal transition.

Carbon nanotube fibers are compatible with Mammalian cells and neurons.