Search Results

Generation and maturation of bone marrow-derived DCs under serum-free conditions

Standard protocols for the generation of murine dendritic cells (DCs) employ medium supplemented with heat-inactivated fetalcalf serum (FCS). Recently, several attempts have been made to avoid serum exposure during DC culture. The impetus for...

Authors: Sung Jung Kim, Betty Diamond

Genome-wide CRISPR Screening to Identify Drivers of TGF-ß-Induced Liver Fibrosis in Human Hepatic Stellate Cells

Liver fibrosis progression in chronic liver disease leads to cirrhosis, liver failure, or hepatocellular carcinoma and often ends in liver transplantation. Even with an increased understanding of liver fibrogenesis and many attempts to generate...

Authors: Shan Yu,*† Matthew Ericson,† Andrea Fanjul,† Derek M. Erion,‡ Maria Paraskevopoulou,‡ Erin N....

SST Peer Reviewed

Point-of-use, automated fabrication of a 3D human liver model supplemented with human adipose microvessels

Advanced in vitro tissue models better reflect healthy and disease tissue conditions in the body. However, complex tissue models are often manufactured using custom solutions and can be challenging to manufacture to scale. Here, we describe the...

Authors: Sarah M Moss , Jillian Schilp, Maya Yaakov , Madison Cook , Erik Schuschke , Brandon Hanke , Hannah...

CARAMBA: a first-in-human clinical trial with SLAMF7 CAR-T cells prepared by virus-free Sleeping Beauty gene transfer to treat multiple myeloma

Clinical development of chimeric antigen receptor (CAR)-T-cell therapy has been enabled by advances in synthetic biology, genetic engineering, clinical-grade manufacturing, and complex logistics to distribute the drug product to treatment sites. A...

Authors: Sabrina Prommersberger , Michael Reiser , Julia Beckmann , Sophia Danhof , Maximilian Amberger ,...

SST Peer Reviewed

Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells

Directed differentiation of human induced pluripotent stem cells (iPSCs) into hepatocytes could provide an unlimited source of liver cells, and therefore holds great promise for regenerative medicine, disease modeling, drug screening and toxicology...

Authors: Rong Li , Yang Zhao , Jeffrey J Yourick , Robert L Sprando , Xiugong Gao

Isogenic cell models of cystic fibrosis-causing variants in natively expressing pulmonary epithelial cells.

Background: Assessment of approved drugs and developmental drug candidates for rare cystic fibrosis (CF)-causing variants of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) requires abundant material from relevant models.Methods:...

Authors: Valley HC, Bukis KM, Bell A, Cheng Y, Wong E, Jordan NJ, Allaire NE, Sivachenko A, Liang F, Bihler...

SST Peer Reviewed

Modeling, optimization, and comparable efficacy of T cell and hematopoietic stem cell gene editing for treating hyper-IgM syndrome

Precise correction of the CD40LG gene in T cells and hematopoietic stem/progenitor cells (HSPC) holds promise for treating X-linked hyper-IgM Syndrome (HIGM1), but its actual therapeutic potential remains elusive. Here, we developed a...

Authors: Valentina Vavassori , Elisabetta Mercuri , Genni E Marcovecchio , Maria C Castiello , Giulia...

Generation of Complex Syngeneic Liver Organoids from Induced Pluripotent Stem Cells to Model Human Liver Pathophysiology

The study of human liver pathophysiology has been hampered for decades by the lack of easily accessible, robust, and representative in vitro models. The discovery of induced pluripotent stem cells (iPSCs)—which can be generated from...

Authors: Claudia Raggi , Silvia Selleri , Marie-Agnes M'Callum , Massimiliano Paganelli

Preclinical Activity of Embryonic Annexin A2-Specific Chimeric Antigen Receptor T Cells Against Ovarian Cancer

Chimeric antigen receptors (CARs) have found clinical success in B cell malignancies, but a dearth of potential targets limits their wider clinical application, especially in solid tumours. Here, we describe the development of an anti-annexin A2 CAR,...

Authors: Leonard Leong , Heng Liang Tan , Simeon Cua , Kylie Su Mei Yong , Qingfeng Chen , Andre Choo

SST Peer Reviewed

Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media

Advances in organoid technology have broadened the number of target diseases and conditions in which human induced pluripotent stem cell (iPSC)-based regenerative medicine can be applied; however, mass production of organoids and the development of...

Authors: Keisuke Sekine , Shimpei Ogawa, Syusaku Tsuzuki, Tatsuya Kobayashi , Kazuki Ikeda, Noriko...

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Generation and maturation of bone marrow-derived DCs under serum-free conditions

Genome-wide CRISPR Screening to Identify Drivers of TGF-ß-Induced Liver Fibrosis in Human Hepatic Stellate Cells

Point-of-use, automated fabrication of a 3D human liver model supplemented with human adipose microvessels

CARAMBA: a first-in-human clinical trial with SLAMF7 CAR-T cells prepared by virus-free Sleeping Beauty gene transfer to treat multiple myeloma

Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells

Isogenic cell models of cystic fibrosis-causing variants in natively expressing pulmonary epithelial cells.

Modeling, optimization, and comparable efficacy of T cell and hematopoietic stem cell gene editing for treating hyper-IgM syndrome

Generation of Complex Syngeneic Liver Organoids from Induced Pluripotent Stem Cells to Model Human Liver Pathophysiology

Preclinical Activity of Embryonic Annexin A2-Specific Chimeric Antigen Receptor T Cells Against Ovarian Cancer

Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media