Search Results

High cell density transient transfection of CHO cellsfor TGF-beta1 expression

High cell densities for transient transfection with polyethyleneimine (PEI) can be usedfor rapid and maximal production of recombinant proteins. High cell densities can beobtained by different cultivation systems, such as batch or perfusion systems....

Authors: Abdalla A. Elshereef, André Jochums Antonina Lavrentieva,Lena Stuckenberg, Thomas Scheper Dörte...

A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing

Bacterial toxins represent a vast reservoir of biochemical diversity that can be repurposed for biomedical applications. Such proteins include a group of predictedinterbacterial toxins of the deaminase superfamily, members of which have found...

Authors: Mok BY, de Moraes MH, Zeng J, Bosch DE, Kotrys AV, Raguram A, Hsu F, Radey MC, Peterson SB, Mootha...

SST Peer Reviewed

Identify Host Factors for SARS-CoV-2 and Common Cold Coronaviruses

The Coronaviridae are a family of viruses that cause disease in humans ranging from mild respiratory infection to potentially lethal acute respiratory distress syndrome. Finding host factors common to multiple coronaviruses could facilitate the...

Authors: Wang R, Simoneau CR, Kulsuptrakul J, Bouhaddou M, Travisano KA, Hayashi JM, Carlson-Stevermer J,...

Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells

Directed differentiation of human induced pluripotent stem cells (iPSCs) into hepatocytes could provide an unlimited source of liver cells, and therefore holds great promise for regenerative medicine, disease modeling, drug screening and toxicology...

Authors: Rong Li , Yang Zhao , Jeffrey J Yourick , Robert L Sprando , Xiugong Gao

Sialic Acid-Engineered IL4–10 Fusion Protein is Bioactiveand Rapidly Cleared from the Circulation

Purpose Modulating sialylation of therapeutic glycoproteinsmay be used to influence their clearance and systemic exposure.We studied the effect of low and high sialylated IL4–10fusion protein (IL4–10 FP) on in vitro and in vivo bioactivity...

Authors: Cristine Steen-Louws & Peter Boross1 & Judith Prado & Jan Meeldijk1 & Jurgen B. Langenhorst &Alwin...

Generation of Complex Syngeneic Liver Organoids from Induced Pluripotent Stem Cells to Model Human Liver Pathophysiology

The study of human liver pathophysiology has been hampered for decades by the lack of easily accessible, robust, and representative in vitro models. The discovery of induced pluripotent stem cells (iPSCs)—which can be generated from...

Authors: Claudia Raggi , Silvia Selleri , Marie-Agnes M'Callum , Massimiliano Paganelli

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

CRISPR-guided DNA cytosine and adenine base editors are widely used for many applications1–4 but primarily create DNA base transitions (that is, pyrimidine-to-pyrimidine or purine-to-purine). Here we describe the engineering of two base editor...

Authors: Kurt IC, Zhou R, Iyer S, Garcia SP, Miller BR, Langner LM, Grünewald J, Joung JK

Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media

Advances in organoid technology have broadened the number of target diseases and conditions in which human induced pluripotent stem cell (iPSC)-based regenerative medicine can be applied; however, mass production of organoids and the development of...

Authors: Keisuke Sekine , Shimpei Ogawa, Syusaku Tsuzuki, Tatsuya Kobayashi , Kazuki Ikeda, Noriko...

Candidate Markers for Stratification and Classification in Rheumatoid Arthritis

Rheumatoid arthritis (RA) is a chronic autoimmune, inflammatory disease, characterizedby synovitis in small- and medium-sized joints and, if not treated early and efficiently, jointdamage, and destruction. RA is a heterogeneous disease with a...

Authors: Lucius Bader, Stein-Erik Gullaksen, Nello Blaser, Morten Brun,Gerd Haga Bringeland, André Sulen,...

Treatment-Induced Tumor Dormancy through YAP-Mediated Transcriptional Reprogramming of the Apoptotic Pathway

Eradicating tumor dormancy that develops following epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) treatment of EGFR-mutant non-small cell lung cancer, is an attractive therapeutic strategy but the mechanisms governing this...

Authors: Kurppa KJ, Liu Y, To C, Zhang T, Fan M, Vajdi A, Knelson EH, Xie Y, Lim K, Cejas P, Portell A,...

Data Type

Category

Research Area

Platform

Topic

Species

Characteristic

Format

Origin