Search Results

Production and characterization of virus-free, CRISPR-CAR T cells capable of inducing solid tumor regression

Background Chimeric antigen receptor (CAR) T cells have demonstrated high clinical response rates against hematological malignancies (e.g., CD19+ cancers) buthave shown limited activity in patients with solid tumors. Recent work showed that precise...

Authors: Mueller KP, Piscopo NJ, Forsberg MH, Saraspe LA, Das A, Russell B, Smerchansky M, Cappabianca D, Shi...

SST Peer Reviewed

Standard operating protocol of hepatic organoid differentiation from human induced pluripotent stem cells

Mature liver organoids are promising cell sources for research to understand the pathology underlying a variety of conditions affecting the liver, including end-stage chronic liver disease. Although several methods exist for the differentiation of...

Authors: Min Jung Kim, Jaeseo Lee , Seon Ju Mun, Myung Jin Son, Jung-Hyun Kim

a2-HS Glycoprotein in Plasma Extracellular Vesicles Inhibits the Osteogenic Differentiation of Human Mesenchymal Stromal Cells In Vitro

Extracellular vesicles (Evs) contain diverse functional proteins, mRNAs, miRNAs, and DNA fragments, are secreted by various types of cells, and play important roles in cellular communication. Here, we show for the first time that plasma Evs inhibited...

Authors: Xiaohua Wu , Mengjun Ma , Peng Wang , Zhongyu Xie , Shan Wang , Hongjun Su , Wen Deng , Pei...

Orthotopic T-cell receptor replacement in primary human T cells using CRISPR-Cas9-mediatedhomology-directed repair

Adoptive T cell therapy using T-cell receptor (TCR)-engineered T cells allows to redirect T cell specificity and to target any antigen of interest. Here, we apply advanced genetic engineering using clustered regularly interspaced short palindromic...

Authors: Carolin Moosmann,* Thomas R. Mueller, Dirk H. Busch, and Kilian Schober

Targeted T cell receptor gene editing provides predictable T cell product function for immunotherapy

Adoptive transfer of T cells expressing a transgenic T cell receptor (TCR) has the potential to revolutionize immunotherapy of infectious diseases and cancer. However, the generation of defined TCR-transgenic T cell medicinal products with...

Authors: Thomas R Müller , Sebastian Jarosch 1, Monika Hammel , Justin Leube , Simon Grassmann , Bettina...

SST Peer Reviewed

Decoy exosomes as a novel biologic reagent to antagonize inflammation

Background: Exosomes are ubiquitous naturally secreted stable nanovesicles that can be engineered to target and deliver novel therapeutics to treat a host of human diseases. Methods: We engineered the surfaces of cell-derived...

Authors: Natalie Duong, Kevin Curley, Annie Brown , Alexander Campanelli , Mai Anh Do , Daniel Levy , Adarsh...

RNAi Transfection Optimized in Primary Naïve B Cells for the Targeted Analysis of Human Plasma Cell Differentiation

Upon antigen recognition, naïve B cells undergo rapid proliferation followed by differentiation to specialized antibody secreting cells (ASCs), called plasma cells. Increased circulating plasma cells are reported in patients with B cell-associated...

Authors: Shih T, De S, Barnes BJ

Modeling retinitis pigmentosa through patient-derived retinal organoids

Human-induced pluripotent stem cells (hiPSCs) can be differentiated into well-structured retinal organoids. In this protocol, we successfully established 3D retinae from patient-derived hiPSCs and built the retinitis pigmentosa model in vitro....

Authors: Yan-Ping Li , Wen-Li Deng , Zi-Bing Jin

SST Peer Reviewed

Human marrow mesenchymal stem cellculture: serum-free medium allows betterexpansion than classical a-MEM medium

The expansion of mesenchymal stem cells (MSCs) strongly depends on the culture conditions and requires medium supplemented with 10-20% fetal calf serum (FCS) to generate relevant numbers of cells. However, the presence of FCS is a major obstacle for...

Authors: Meuleman N, Tondreau T, Delforge A, Dejeneffe M, Massy M,Libertalis M, Bron D, Lagneaux L.

A functional genetic toolbox for human tissue-derived organoids

Human organoid systems recapitulate key features of organs offering platforms for modelling developmental biology and disease. Tissue-derived organoids have been widely used to study the impact of extrinsic niche factors on stem cells. However, they...

Authors: Dawei Sun , Lewis Evans , Francesca Perrone , Vanesa Sokleva , Kyungtae Lim , Saba Rezakhani ,...

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Production and characterization of virus-free, CRISPR-CAR T cells capable of inducing solid tumor regression

Standard operating protocol of hepatic organoid differentiation from human induced pluripotent stem cells

a2-HS Glycoprotein in Plasma Extracellular Vesicles Inhibits the Osteogenic Differentiation of Human Mesenchymal Stromal Cells In Vitro

Orthotopic T-cell receptor replacement in primary human T cells using CRISPR-Cas9-mediatedhomology-directed repair

Targeted T cell receptor gene editing provides predictable T cell product function for immunotherapy

Decoy exosomes as a novel biologic reagent to antagonize inflammation

RNAi Transfection Optimized in Primary Naïve B Cells for the Targeted Analysis of Human Plasma Cell Differentiation

Modeling retinitis pigmentosa through patient-derived retinal organoids

Human marrow mesenchymal stem cellculture: serum-free medium allows betterexpansion than classical a-MEM medium

A functional genetic toolbox for human tissue-derived organoids