Search Results

Search-and-replace genome editing without double-strand breaks or donor DNA.

Most genetic variants that contribute to disease1 are challenging to correct efficiently and without excess byproducts2-5. Here we describe prime editing, a versatile and precise genome editing method that directly writes new genetic information into...

Authors: Anzalone AV1,2,3, Randolph PB1,2,3, Davis JR1,2,3, Sousa AA1,2,3, Koblan LW1,2,3, Levy JM1,2,3, Chen...

SST Peer Reviewed

Cancer invasion regulates vascular complexity in a three-dimensional biomimetic model.

INTRODUCTION:There is a growing appreciation for including a complex, vascularised stroma in three-dimensional (3D) tumour models to recapitulate the native tumour microenvironment in situ.METHODS:Using a compartmentalised, biomimetic, 3D cancer...

Authors: Pape J1, Magdeldin T1, Ali M2, Walsh C2, Lythgoe M2, Emberton M3, Cheema U4.

SST Peer Reviewed

Phosphatidylethanolamine and Phosphatidylcholine Biosynthesis by the Kennedy Pathway Occurs at Different Sites in Trypanosoma Brucei

Phosphatidylethanolamine (PE) and phosphatidylcholine (PC) are among the most abundant phospholipids in biological membranes. In many eukaryotes, the CDP-ethanolamine and CDP-choline branches of the Kennedy pathway represent major and often essential...

Authors: Luce Farine 1 , Moritz Niemann 3 , André...

SST Peer Reviewed

Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo

During the outbreak of SARS in 2002/3, a prototype virus was isolated from a patient in Frankfurt/Germany (strain Frankfurt-1). As opposed to all other SARS-Coronavirus strains, Frankfurt-1 has a 45-nucleotide deletion in the transmembrane domain of...

Authors: Susanne Pfefferle, Verena Krähling, Vanessa Ditt, Klaus Grywna, Elke Mühlberger, Christian Drosten

SST Peer Reviewed

Triptolide mitigates radiation-induced pneumonitis via inhibition of alveolar macrophages and related inflammatory molecules

Ionizing radiation-induced pulmonary injury is a major limitation of radiotherapy for thoracic tumors. We have demonstrated that triptolide (TPL) could alleviate IR-induced pneumonia and pulmonary fibrosis. In this study, we explored the underlying...

Authors: Chun Chen, Shanmin Yang, Mei Zhang, Zhenhuan Zhang, Steven B Zhang, Bing Wu, Jinsheng Hong, Weijian...

A 3D Heterotypic Multicellular Tumor Spheroid Assay Platform to Discriminate Drug Effects on Stroma versus Cancer Cells.

Three-dimensional (3D) cell culture models are thought to mimic the physiological and pharmacological properties of tissues in vivo more accurately than two-dimensional cultures on plastic dishes. For the development of cancer therapies, 3D spheroid...

Authors: Weydert Z, Lal-Nag M, Mathews-Greiner L, Thiel C, Cordes H, Küpfer L, Guye P, Kelm JM, Ferrer M.

SST Peer Reviewed

Methylation in HT22 cells and primary hippocampal neurons with and without isoflurane exposure

Background: Epigenetic modulation may play a role in anesthesia related phenotypes, such as cognitive impairment or memory loss, especially with exposure to anesthetics in the vulnerable phase of brain development. While isoflurane anesthesia...

Authors: Stefanie Klenke , Christian Specking , Maike Stegen , Andrea Engler , Jürgen Peters

Enolate-forming compounds provide protection from platinum neurotoxicity

Cisplatin (CisPt) and other platinum (Pt)-based antineoplastic drugs (e.g., carboplatin, oxaliplatin) are highly effective and widely used in the treatment of solid tumors in both pediatric and adult patients. Although considered to be life-saving as...

Authors: Geohagen BC, Weiser DA, Loeb DM, Nordstroem LU, LoPachin RM

Central IRAK-4 kinase inhibition for the treatment of pain following nerve injury in rats

There is ample evidence for the role of the immune system in developing chronic pain following peripheral nerve injury. Especially Toll-like receptors (TLRs) and their associated signaling components and pro-inflammatory cytokines such as IL-1ß,...

Authors: Katrien Pletinckx , Duygu Krings , André Welbers , David A Rider , Torsten R Dunkern

Pharmacological or genetic depletion of senescent astrocytes prevents whole brain irradiation-induced impairment of neurovascular coupling responses protecting cognitive function in mice

Whole brain irradiation (WBI, also known as whole brain radiation therapy or WBRT) is a mainstream therapy for patients with identifiable brain metastases and as a prophylaxis for microscopic malignancies. WBI accelerates brain...

Authors: Yabluchanskiy A, Tarantini S, Balasubramanian P, Kiss T, Csipo T, Fülöp GA, Lipecz A, Ahire...

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Search-and-replace genome editing without double-strand breaks or donor DNA.

Cancer invasion regulates vascular complexity in a three-dimensional biomimetic model.

Phosphatidylethanolamine and Phosphatidylcholine Biosynthesis by the Kennedy Pathway Occurs at Different Sites in Trypanosoma Brucei

Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo

Triptolide mitigates radiation-induced pneumonitis via inhibition of alveolar macrophages and related inflammatory molecules

A 3D Heterotypic Multicellular Tumor Spheroid Assay Platform to Discriminate Drug Effects on Stroma versus Cancer Cells.

Methylation in HT22 cells and primary hippocampal neurons with and without isoflurane exposure

Enolate-forming compounds provide protection from platinum neurotoxicity

Central IRAK-4 kinase inhibition for the treatment of pain following nerve injury in rats

Pharmacological or genetic depletion of senescent astrocytes prevents whole brain irradiation-induced impairment of neurovascular coupling responses protecting cognitive function in mice