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An ShRNA Based Genetic Screen Identified Sesn2 as a Potential Tumor Suppressor in Lung Cancer via Suppression of Akt-mTOR-p70S6K Signaling.

BACKGROUND: Lung cancer is emerging rapidly as the leading death cause in Chinese cancer patients. The causal factors for Chinese lung cancer development remain largely unclear. Here we employed an shRNA library-based loss-of-function screen in a...

Authors: Xu H, Sun H, Zhang H, Liu J, Fan F, Li Y, Ning X, Sun Y, Dai S, Liu B, Gao M, Fu S, Zhou C

SST Peer Reviewed

Regulation of the Flt3 Gene in Haematopoietic Stem and Early Progenitor Cells

The MYB transcription factor plays critical roles in normal and malignant haematopoiesis. We previously showed that MYB was a direct activator of FLT3 expression within the context of acute myeloid leukaemia. During normal haematopoiesis, increasing...

Authors: Giacomo Volpe, Mary Clarke, Paloma Garcìa, David Scott Walton, Alexandros Vegiopoulos, Walter Del...

SST Peer Reviewed

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system

Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P....

Authors: Mehdi Ghorbal, Molly Gorman, Cameron Ross Macpherson, Rafael Miyazawa Martins, Artur Scherf,...

SST Peer Reviewed

Cytokine-Induced Loss of Glucocorticoid Function: Effect of Kinase Inhibitors, Long- Acting ß2-Adrenoceptor Agonist and Glucocorticoid Receptor Ligands

Acting on the glucocorticoid receptor (NR3C1), glucocorticoids are widely used to treat inflammatory diseases. However, glucocorticoid resistance often leads to suboptimal asthma control. Since glucocorticoid-induced gene expression contributes to...

Authors: Christopher F. Rider, Suharsh Shah, Anna Miller-Larsson, Mark A. Giembycz, Robert Newton1

SST Peer Reviewed

Current Protocols in Stem Cell Biology: CRISPR-Mediated Gene Targeting of Human Induced Pluripotent Stem Cells.

CRISPR/Cas9 nuclease systems can create double-stranded DNA breaks at specific sequences to efficiently and precisely disrupt, excise, mutate, insert, or replace genes. However, human embryonic stem cells and induced pluripotent stem cells (iPSCs)...

Authors: SM Byrne, GM Church

SST Peer Reviewed

Efficient generation of integration-free ips cells from human adult peripheral blood using BCL-XL together with Yamanaka factors.

The ability to efficiently generate integration-free induced pluripotent stem cells (iPSCs) from the most readily available source-peripheral blood-has the potential to expedite the advances of iPSC-based therapies. We have successfully generated...

Authors: Su RJ, Baylink DJ, Neises A, Kiroyan JB, Meng X, Payne KJ, Tschudy-Seney B, Duan Y, Appleby N,...

SST Peer Reviewed

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing.

Monomeric CRISPR-Cas9 nucleases are widely used for targeted genome editing but can induce unwanted off-target mutations with high frequencies. Here we describe dimeric RNA-guided FokI nucleases (RFNs) that can recognize extended sequences and edit...

Authors: Shengdar Q. Tsai, Nicolas Wyvekens, Cyd Khayter, Jennifer A. Foden, Vishal Thapar, Deepak Reyon,...

SST Peer Reviewed

Generation of a human airway epithelium derived basal cell line with multipotent differentiation capacity

BACKGROUND: As the multipotent progenitor population of the airway epithelium, human airway basal cells (BC) replenish the specialized differentiated cell populations of the mucociliated airway epithelium during physiological turnover and repair....

Authors: Walters MS, Gomi K, Ashbridge B, Moore MA, Arbelaez V, Heldrich J, Ding BS, Rafii S, Staudt MR,...

SST Peer Reviewed

Human lymphoblastoid B-cell lines reprogrammed to EBV-free induced pluripotent stem cells

Generation of patient-specific induced pluripotent cells (iPSCs) holds great promise for regenerative medicine. Epstein-Barr virus immortalized lymphoblastoid B-cell lines (LCLs) can be generated from a minimal amount of blood and are banked...

Authors: Deepika Rajesh, Sarah J. Dickerson, Junying Yu, Matthew E. Brown, James A. Thomson, and Nicholas J....

SST Peer Reviewed

Introduction of germline residues improves the stability of anti-HIV mAb 2G12-IgM

Immunoglobulins M (IgMs) are gaining increasing attention as biopharmaceuticals since their multivalent mode of binding can give rise to high avidity. Furthermore, IgMs are potent activators of the complement system. However, they are frequently...

Authors: Chromikova V, Mader A, Hofbauer S, Göbl C, Madl T, Gach JS, Bauernfried S, Furtmüller PG, Forthal...

SST Peer Reviewed

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An ShRNA Based Genetic Screen Identified Sesn2 as a Potential Tumor Suppressor in Lung Cancer via Suppression of Akt-mTOR-p70S6K Signaling.

Regulation of the Flt3 Gene in Haematopoietic Stem and Early Progenitor Cells

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system

Cytokine-Induced Loss of Glucocorticoid Function: Effect of Kinase Inhibitors, Long- Acting ß2-Adrenoceptor Agonist and Glucocorticoid Receptor Ligands

Current Protocols in Stem Cell Biology: CRISPR-Mediated Gene Targeting of Human Induced Pluripotent Stem Cells.

Efficient generation of integration-free ips cells from human adult peripheral blood using BCL-XL together with Yamanaka factors.

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing.

Generation of a human airway epithelium derived basal cell line with multipotent differentiation capacity

Human lymphoblastoid B-cell lines reprogrammed to EBV-free induced pluripotent stem cells

Introduction of germline residues improves the stability of anti-HIV mAb 2G12-IgM