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Efficient and versatile CRISPR engineering of human neurons in culture to model neurological disorders.

The recent identification of multiple new genetic causes of neurological disorders highlights the need for model systems that give experimental access to the underlying biology. In particular, the ability to couple disease-causing mutations with...

Authors: Shah RR1, Cholewa-Waclaw J1, Davies FCJ2, Paton KM1, Chaligne R3, Heard E3, Abbott CM2, Bird AP1.

SST Peer Reviewed

CRISPR-Cas9 genome engineering of primary CD4+ T cells for the interrogation of HIV-host factor interactions.

CRISPR-Cas9 gene-editing strategies have revolutionized our ability to engineer the human genome for robust functional interrogation of complex biological processes. We have recently adapted this technology for use in primary human CD4+ T cells to...

Authors: Hultquist JF, Hiatt J, Schumann K, McGregor MJ, Roth TL, Haas P, Doudna JA, Marson A, Krogan NJ.

SST Peer Reviewed

Human marrow mesenchymal stem cellculture: serum-free medium allows betterexpansion than classical a-MEM medium

The expansion of mesenchymal stem cells (MSCs) strongly depends on the culture conditions and requires medium supplemented with 10-20% fetal calf serum (FCS) to generate relevant numbers of cells. However, the presence of FCS is a major obstacle for...

Authors: Meuleman N, Tondreau T, Delforge A, Dejeneffe M, Massy M,Libertalis M, Bron D, Lagneaux L.

The Replication of a Mouse Adapted SARS-CoV in a Mouse Cell Line Stably Expressing the Murine SARS-CoV Receptor mACE2 Efficiently Induces the Expression of Proinflammatory Cytokines

Infection of conventional mice with a mouse adapted (MA15) severe acute respiratory syndrome (SARS) coronavirus (CoV) reproduces many aspects of human SARS such as pathological changes in lung, viremia, neutrophilia, and lethality. However,...

Authors: Jose A Regla-Nava 1 , Jose M Jimenez-Guardeño, Jose L Nieto-Torres, Thomas...

SST Peer Reviewed

HDAC inhibitor AR-42 decreases CD44 expression and sensitizes myeloma cells to lenalidomide.

Multiple myeloma (MM) is a hematological malignancy of plasma cells in the bone marrow. Despite multiple treatment options, MM is inevitably associated with drug resistance and poor outcomes. Histone deacetylase inhibitors (HDACi's) are promising...

Authors: Canella A, Cordero Nieves H, Sborov DW, Cascione L, Radomska HS, Smith E, Stiff A, Consiglio J,...

Liraglutide, a glucagon-like peptide-1 analog, inhibits high glucose-induced oxidative stress and apoptosis in neonatal rat cardiomyocytes

Cardiomyocyte apoptosis serves an important role in diabetic cardiomyopathy. Liraglutide, a glucagon-like peptide-1 analog, has been indicated to exert a cardioprotective effect. However, the role of liraglutide on cardiomyocyte apoptosis in...

Authors: LIHUI ZHANG, CAIGE LI, QIUXIAO ZHU, NA LI and HONG ZHOU

Enhanced transfection of cell lines from Atlantic salmon through nucoleofection and antibiotic selection.

BACKGROUND:Cell lines from Atlantic salmon kidney have made it possible to culture and study infectious salmon anemia virus (ISAV), an aquatic orthomyxovirus affecting farmed Atlantic salmon. However, transfection of these cells using calcium...

Authors: Schiøtz BL1, Rosado EG, Baekkevold ES, Lukacs M, Mjaaland S, Sindre H, Grimholt U, Gjøen T.

SST Peer Reviewed

Targeted delivery of lysosomalenzymes to the endocyticcompartment in human cells usingengineered extracellular vesicles

Targeted delivery of lysosomal enzymes to the endocytic compartment of human cells represents a transformative technology for treating a large family of lysosomal storage diseases (LSDs). Gaucher disease is one of the most common types of LSDs caused...

Authors: Mai Anh Do , Daniel Levy , Annie Brown , Gerard Marriott , Biao Lu

Cell wall hydrolase as a surface-associated protein target for the specific detection of Lactobacillus rhamnosus using flow cytometry

Flow cytometry (FC) is a very popular technique for to the study of different eukaryotic cell phenotypes. In addition, FC has many applications in the field of food microbiology, although the few number of specific molecules that can be potentially...

Authors: Raquel Marcos-Fernández, Lorena Ruiz, Aitor Blanco-Míguez, Abelardo Margolles, Borja Sánchez

Optimization of Normal Human Bronchial Epithelial (NHBE) Cell 3D Cultures for in vitro Lung Model Studies.

Robust in vitro lung models are required for risk assessment to measure key events leading to respiratory diseases. Primary normal human bronchial epithelial cells (NHBE) represent a good lung model but obtaining well-differentiated 3D cultures can...

Authors: Rayner RE, Makena P, Prasad GL, Cormet-Boyaka E.

SST Peer Reviewed

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Efficient and versatile CRISPR engineering of human neurons in culture to model neurological disorders.

CRISPR-Cas9 genome engineering of primary CD4+ T cells for the interrogation of HIV-host factor interactions.

Human marrow mesenchymal stem cellculture: serum-free medium allows betterexpansion than classical a-MEM medium

The Replication of a Mouse Adapted SARS-CoV in a Mouse Cell Line Stably Expressing the Murine SARS-CoV Receptor mACE2 Efficiently Induces the Expression of Proinflammatory Cytokines

HDAC inhibitor AR-42 decreases CD44 expression and sensitizes myeloma cells to lenalidomide.

Liraglutide, a glucagon-like peptide-1 analog, inhibits high glucose-induced oxidative stress and apoptosis in neonatal rat cardiomyocytes

Enhanced transfection of cell lines from Atlantic salmon through nucoleofection and antibiotic selection.

Targeted delivery of lysosomalenzymes to the endocyticcompartment in human cells usingengineered extracellular vesicles

Cell wall hydrolase as a surface-associated protein target for the specific detection of Lactobacillus rhamnosus using flow cytometry

Optimization of Normal Human Bronchial Epithelial (NHBE) Cell 3D Cultures for in vitro Lung Model Studies.