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High cell density transient transfection of CHO cellsfor TGF-beta1 expression

High cell densities for transient transfection with polyethyleneimine (PEI) can be usedfor rapid and maximal production of recombinant proteins. High cell densities can beobtained by different cultivation systems, such as batch or perfusion systems....

Authors: Abdalla A. Elshereef, André Jochums Antonina Lavrentieva,Lena Stuckenberg, Thomas Scheper Dörte...

SARS-CoV-2 launches a unique transcriptional signature from in vitro, ex vivo, and in vivo systems

One of the greatest threats to humanity is the emergence of a pandemic virus. Among those with the greatest potential for such an event include influenza viruses and coronaviruses. In the last century alone, we have observed four major influenza A...

Authors: Daniel Blanco-Melo, Benjamin E. Nilsson-Payant, Wen-Chun Liu, Rasmus Møller, Maryline Panis, David...

SST Peer Reviewed

Quantitative analysis of the CD4+ T cell response to therapeutic antibodies in healthy donors using a novel T cell:PBMC assay.

Many biopharmaceuticals (BPs) are known to be immunogenic in the clinic, which can result in modified pharmacokinetics, reduced efficacy, allergic reactions and anaphylaxis. During recent years, several technologies to predict immunogenicity have...

Authors: Schultz HS1, Reedtz-Runge SL2, Bäckström BT1, Lamberth K1, Pedersen CR3, Kvarnhammar AM1; ABIRISK...

SST Peer Reviewed

A p53-dependent response limits the viability of mammalian haploid cells.

The recent development of haploid cell lines has facilitated forward genetic screenings in mammalian cells. These lines include near-haploid human cell lines isolated from a patient with chronic myelogenous leukemia (KBM7 and HAP1), as well as...

Authors: Olbrich T, Mayor-Ruiz C, Vega-Sendino M, Gomez C, Ortega S, Ruiz S, Fernandez-Capetillo O.

Virological Assessment of Hospitalized Patients With COVID-2019

Coronavirus disease 2019 (COVID-19) is an acute respiratory tract infection that emerged in late 20191,2. Initial outbreaks in China involved 13.8% cases with severe, and 6.1% with critical courses3. This severe presentation corresponds to the usage...

Authors: Roman Wölfel 1 , Victor M Corman 2 , Wolfgang...

SST Peer Reviewed

Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity.

We recently developed base editing, the programmable conversion of target C:G base pairs to T:A without inducing double-stranded DNA breaks (DSBs) or requiring homology-directed repair using engineered fusions of Cas9 variants and cytidine...

Authors: Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW, Kim YB, Badran AH, Liu DR.

Sialic Acid-Engineered IL4–10 Fusion Protein is Bioactiveand Rapidly Cleared from the Circulation

Purpose Modulating sialylation of therapeutic glycoproteinsmay be used to influence their clearance and systemic exposure.We studied the effect of low and high sialylated IL4–10fusion protein (IL4–10 FP) on in vitro and in vivo bioactivity...

Authors: Cristine Steen-Louws & Peter Boross1 & Judith Prado & Jan Meeldijk1 & Jurgen B. Langenhorst &Alwin...

Candidate Markers for Stratification and Classification in Rheumatoid Arthritis

Rheumatoid arthritis (RA) is a chronic autoimmune, inflammatory disease, characterizedby synovitis in small- and medium-sized joints and, if not treated early and efficiently, jointdamage, and destruction. RA is a heterogeneous disease with a...

Authors: Lucius Bader, Stein-Erik Gullaksen, Nello Blaser, Morten Brun,Gerd Haga Bringeland, André Sulen,...

Adeno-Associated Virus (AAV) as a Vector for Gene Therapy

Abstract There has been a resurgence in gene therapyefforts that is partly fueled by the identification andunderstanding of new gene delivery vectors. Adeno-associatedvirus (AAV) is a non-enveloped virus that can beengineered to deliver DNA to target...

Authors: Michael F. Naso, Brian Tomkowicz, William L. Perry III, William R. Strohl

Gene-edited stem cells enable CD33-directed immune therapy for myeloid malignancies.

Antigen-directed immunotherapies for acute myeloid leukemia (AML), such as chimeric antigen receptor T cells (CAR-Ts) or antibody-drug conjugates (ADCs), are associated with severe toxicities due to the lack of unique targetable antigens that can...

Authors: Borot F, Wang H, Ma Y, Jafarov T, Raza A, Ali AM, Mukherjee S.

SST Peer Reviewed

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High cell density transient transfection of CHO cellsfor TGF-beta1 expression

SARS-CoV-2 launches a unique transcriptional signature from in vitro, ex vivo, and in vivo systems

Quantitative analysis of the CD4+ T cell response to therapeutic antibodies in healthy donors using a novel T cell:PBMC assay.

A p53-dependent response limits the viability of mammalian haploid cells.

Virological Assessment of Hospitalized Patients With COVID-2019

Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity.

Sialic Acid-Engineered IL4–10 Fusion Protein is Bioactiveand Rapidly Cleared from the Circulation

Candidate Markers for Stratification and Classification in Rheumatoid Arthritis

Adeno-Associated Virus (AAV) as a Vector for Gene Therapy

Gene-edited stem cells enable CD33-directed immune therapy for myeloid malignancies.