Search Results

CRISPR/Cas9-Based Gene Engineering of Human Natural Killer Cells: Protocols for Knockout and Readouts to Evaluate Their Efficacy

Natural killer (NK) cells are cytotoxic lymphocytes of our immune system with the ability to identify and kill certain virally infected and tumor-transformed cells. During the past 15 years, it has become increasingly clear that NK cells are involved...

Authors: Mélanie Lambert , Caroline Leijonhufvud , Filip Segerberg , J Joseph Melenhorst , Mattias Carlsten

HDAC inhibitor AR-42 decreases CD44 expression and sensitizes myeloma cells to lenalidomide.

Multiple myeloma (MM) is a hematological malignancy of plasma cells in the bone marrow. Despite multiple treatment options, MM is inevitably associated with drug resistance and poor outcomes. Histone deacetylase inhibitors (HDACi's) are promising...

Authors: Canella A, Cordero Nieves H, Sborov DW, Cascione L, Radomska HS, Smith E, Stiff A, Consiglio J,...

Sialic Acid-Engineered IL4–10 Fusion Protein is Bioactiveand Rapidly Cleared from the Circulation

Purpose Modulating sialylation of therapeutic glycoproteinsmay be used to influence their clearance and systemic exposure.We studied the effect of low and high sialylated IL4–10fusion protein (IL4–10 FP) on in vitro and in vivo bioactivity...

Authors: Cristine Steen-Louws & Peter Boross1 & Judith Prado & Jan Meeldijk1 & Jurgen B. Langenhorst &Alwin...

PU.1 Controls Fibroblast Polarization and Tissue Fibrosis

Fibroblasts are polymorphic cells with pleiotropic roles in organ morphogenesis, tissue homeostasis and immune responses. In fibrotic diseases, fibroblasts synthesize abundant amounts of extracellular matrix, which induces scarring and organ failure....

Authors: Thomas Wohlfahrt , Simon Rauber , Steffen Uebe , Markus Luber , Alina Soare , Arif Ekici , Stefanie...

Enhanced transfection of cell lines from Atlantic salmon through nucoleofection and antibiotic selection.

BACKGROUND:Cell lines from Atlantic salmon kidney have made it possible to culture and study infectious salmon anemia virus (ISAV), an aquatic orthomyxovirus affecting farmed Atlantic salmon. However, transfection of these cells using calcium...

Authors: Schiøtz BL1, Rosado EG, Baekkevold ES, Lukacs M, Mjaaland S, Sindre H, Grimholt U, Gjøen T.

SST Peer Reviewed

microRNA-184 Is Induced by Store-Operated Calcium Entry and Regulates Early Keratinocyte Differentiation

Extracellular calcium (Ca2+ ) and store-operated Ca2+ entry (SOCE) govern homoeostasis in the mammalian epidermis. Multiple microRNAs (miRNA) also regulate epidermal differentiation, and raised external Ca2+ modulates the expression of several such...

Authors: Adam Richardson , Andrew K Powell , Darren W Sexton , Jason L Parsons , Nick J Reynolds , Kehinde...

Optimization of Normal Human Bronchial Epithelial (NHBE) Cell 3D Cultures for in vitro Lung Model Studies.

Robust in vitro lung models are required for risk assessment to measure key events leading to respiratory diseases. Primary normal human bronchial epithelial cells (NHBE) represent a good lung model but obtaining well-differentiated 3D cultures can...

Authors: Rayner RE, Makena P, Prasad GL, Cormet-Boyaka E.

SST Peer Reviewed

Successfully Transfected Primary Peripherally Mobilized Human CD34+ Hematopoietic Stem and Progenitor Cells (HSPCs) Demonstrate Increased Susceptibility to Retroviral Infection

Transfection, the process of introducing purified nucleic acids into cells, and viral transduction, viral-mediated nucleic acid transfer, are two commonly utilized techniques for gene delivery in the research setting. Transfection allows purified...

Authors: Jeffrey Sebrow , Stephen P Goff, Daniel O Griffin

SST Peer Reviewed

Candidate Markers for Stratification and Classification in Rheumatoid Arthritis

Rheumatoid arthritis (RA) is a chronic autoimmune, inflammatory disease, characterizedby synovitis in small- and medium-sized joints and, if not treated early and efficiently, jointdamage, and destruction. RA is a heterogeneous disease with a...

Authors: Lucius Bader, Stein-Erik Gullaksen, Nello Blaser, Morten Brun,Gerd Haga Bringeland, André Sulen,...

A pneumonia outbreak associated with a new coronavirus of probable bat origin

Since the outbreak of severe acute respiratory syndrome (SARS) 18 years ago, a large number of SARS-related coronaviruses (SARSr-CoVs) have been discovered in their natural reservoir host, bats1–4. Previous studies have shown that some bat...

Authors: Zhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W, Si HR, Zhu Y, Li B, Huang CL, Chen HD, Chen J, Luo...

SST Peer Reviewed

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CRISPR/Cas9-Based Gene Engineering of Human Natural Killer Cells: Protocols for Knockout and Readouts to Evaluate Their Efficacy

HDAC inhibitor AR-42 decreases CD44 expression and sensitizes myeloma cells to lenalidomide.

Sialic Acid-Engineered IL4–10 Fusion Protein is Bioactiveand Rapidly Cleared from the Circulation

PU.1 Controls Fibroblast Polarization and Tissue Fibrosis

Enhanced transfection of cell lines from Atlantic salmon through nucoleofection and antibiotic selection.

microRNA-184 Is Induced by Store-Operated Calcium Entry and Regulates Early Keratinocyte Differentiation

Optimization of Normal Human Bronchial Epithelial (NHBE) Cell 3D Cultures for in vitro Lung Model Studies.

Successfully Transfected Primary Peripherally Mobilized Human CD34+ Hematopoietic Stem and Progenitor Cells (HSPCs) Demonstrate Increased Susceptibility to Retroviral Infection

Candidate Markers for Stratification and Classification in Rheumatoid Arthritis

A pneumonia outbreak associated with a new coronavirus of probable bat origin