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Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells.

Visceral fat accumulation as observed in Crohn's disease and obesity is linked to chronic gut inflammation, suggesting that accumulation of gut adipocytes can trigger local inflammatory signaling. However, direct interactions between intestinal...

Authors: Takahashi Y1, Sato S2, Kurashima Y3, Lai CY4, Otsu M4, Hayashi M5, Yamaguchi T5, Kiyono H6.

PU.1 Controls Fibroblast Polarization and Tissue Fibrosis

Fibroblasts are polymorphic cells with pleiotropic roles in organ morphogenesis, tissue homeostasis and immune responses. In fibrotic diseases, fibroblasts synthesize abundant amounts of extracellular matrix, which induces scarring and organ failure....

Authors: Thomas Wohlfahrt , Simon Rauber , Steffen Uebe , Markus Luber , Alina Soare , Arif Ekici , Stefanie...

Efficient and versatile CRISPR engineering of human neurons in culture to model neurological disorders.

The recent identification of multiple new genetic causes of neurological disorders highlights the need for model systems that give experimental access to the underlying biology. In particular, the ability to couple disease-causing mutations with...

Authors: Shah RR1, Cholewa-Waclaw J1, Davies FCJ2, Paton KM1, Chaligne R3, Heard E3, Abbott CM2, Bird AP1.

SST Peer Reviewed

microRNA-184 Is Induced by Store-Operated Calcium Entry and Regulates Early Keratinocyte Differentiation

Extracellular calcium (Ca2+ ) and store-operated Ca2+ entry (SOCE) govern homoeostasis in the mammalian epidermis. Multiple microRNAs (miRNA) also regulate epidermal differentiation, and raised external Ca2+ modulates the expression of several such...

Authors: Adam Richardson , Andrew K Powell , Darren W Sexton , Jason L Parsons , Nick J Reynolds , Kehinde...

Nucleofection with Plasmid DNA for CRISPR/Cas9-Mediated Inactivation of Programmed Cell Death Protein 1 in CD133-Specific CAR T Cells

CRISPR/Cas9-mediated programmed cell death protein 1 (PD-1) disruption in chimeric antigen receptor (CAR) T cells could be an appealing choice to improve the therapeutic efficacy of CAR T cells in an immunosuppressive tumor microenvironment. In most...

Authors: Hu B, Zou Y, Zhang L, Tang J, Niedermann G, Firat E, Huang X, Zhu X.

SST Peer Reviewed

CRISPR-Cas9 genome engineering of primary CD4+ T cells for the interrogation of HIV-host factor interactions.

CRISPR-Cas9 gene-editing strategies have revolutionized our ability to engineer the human genome for robust functional interrogation of complex biological processes. We have recently adapted this technology for use in primary human CD4+ T cells to...

Authors: Hultquist JF, Hiatt J, Schumann K, McGregor MJ, Roth TL, Haas P, Doudna JA, Marson A, Krogan NJ.

SST Peer Reviewed

Successfully Transfected Primary Peripherally Mobilized Human CD34+ Hematopoietic Stem and Progenitor Cells (HSPCs) Demonstrate Increased Susceptibility to Retroviral Infection

Transfection, the process of introducing purified nucleic acids into cells, and viral transduction, viral-mediated nucleic acid transfer, are two commonly utilized techniques for gene delivery in the research setting. Transfection allows purified...

Authors: Jeffrey Sebrow , Stephen P Goff, Daniel O Griffin

SST Peer Reviewed

Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function.

Human T cells are central effectors of immunity and cancer immunotherapy. CRISPR-based functional studies in T cells could prioritize novel targets for drug development and improve the design of genetically reprogrammed cell-based therapies. However,...

Authors: Shifrut E, Carnevale J, Tobin V, Roth TL, Woo JM, Bui CT, Li PJ, Diolaiti ME, Ashworth A, Marson...

SST Peer Reviewed

HDAC inhibitor AR-42 decreases CD44 expression and sensitizes myeloma cells to lenalidomide.

Multiple myeloma (MM) is a hematological malignancy of plasma cells in the bone marrow. Despite multiple treatment options, MM is inevitably associated with drug resistance and poor outcomes. Histone deacetylase inhibitors (HDACi's) are promising...

Authors: Canella A, Cordero Nieves H, Sborov DW, Cascione L, Radomska HS, Smith E, Stiff A, Consiglio J,...

Enhanced transfection of cell lines from Atlantic salmon through nucoleofection and antibiotic selection.

BACKGROUND:Cell lines from Atlantic salmon kidney have made it possible to culture and study infectious salmon anemia virus (ISAV), an aquatic orthomyxovirus affecting farmed Atlantic salmon. However, transfection of these cells using calcium...

Authors: Schiøtz BL1, Rosado EG, Baekkevold ES, Lukacs M, Mjaaland S, Sindre H, Grimholt U, Gjøen T.

SST Peer Reviewed

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Topic

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Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells.

PU.1 Controls Fibroblast Polarization and Tissue Fibrosis

Efficient and versatile CRISPR engineering of human neurons in culture to model neurological disorders.

microRNA-184 Is Induced by Store-Operated Calcium Entry and Regulates Early Keratinocyte Differentiation

Nucleofection with Plasmid DNA for CRISPR/Cas9-Mediated Inactivation of Programmed Cell Death Protein 1 in CD133-Specific CAR T Cells

CRISPR-Cas9 genome engineering of primary CD4+ T cells for the interrogation of HIV-host factor interactions.

Successfully Transfected Primary Peripherally Mobilized Human CD34+ Hematopoietic Stem and Progenitor Cells (HSPCs) Demonstrate Increased Susceptibility to Retroviral Infection

Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function.

HDAC inhibitor AR-42 decreases CD44 expression and sensitizes myeloma cells to lenalidomide.

Enhanced transfection of cell lines from Atlantic salmon through nucoleofection and antibiotic selection.