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Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites.

Transfection is a technical process through which genetic material, such as DNA and double-stranded RNA, are delivered into cells to modify the gene of interest. Currently, transgenic technology is becoming an indispensable tool for the study of...

Authors: Duan C1, Tang X1, Hu D1, Zhang S1, Liu J1, Bi F1, Hao Z1, Suo J1, Yu Y1, Wang M1, Sun P1, Du L2, Suo...

IRF2 is a master regulator of human keratinocyte stem cell fate

Resident adult epithelial stem cells maintain tissue homeostasis by balancing self-renewal and differentiation. The stem cell potential of human epidermal keratinocytes is retained in vitro but lost over time suggesting extrinsic and intrinsic...

Authors: Mercado N, Schutzius G, Kolter C, Estoppey D, Bergling S, Roma G, Gubser Keller C, Nigsch F, Salathe...

ALCAM Mediates DC MigrationThrough Afferent Lymphatics andPromotes Allospecific ImmuneReactions

Activated leukocyte cell adhesion molecule (ALCAM, CD166) is a cell adhesion moleculeof the immunoglobulin superfamily and has been implicated in diverse pathophysiologicalprocesses including T cell activation, leukocyte trafficking, and...

Authors: Ann-Helen Willrodt, Ann-Charlott Salabarria, Philipp Schineis, Desislava Ignatova,Morgan Campbell...

Design and characterisation of a novelinterleukin-15 receptor alpha fusion proteinand analysis of interleukin-15 complexation

Interleukin-15 (IL15) is one of the most important cytokines currently being considered forcancer therapy applications. It is thought that by administering IL15 in complex with its cognatereceptor alpha chain (IL15Ra) its biological activity could be...

Authors: Anja Sophie Schmid, Dario NeriI

Generation of CCR5-ablated Human Induced Pluripotent Stem Cells as a Therapeutic Approach for Immune-mediated Diseases

C-C chemokine receptor type 5 (CCR5) is a receptor for some pro-inflammatory chemokines which plays important roles in immunological disorder and host responses to infectious agents. Additionally, the prognosis of some immune-mediated diseases in the...

Authors: Negin Hosseini Rouzbahani , Saeid Kaviani , Mohammad Vasei , Masoud Soleimani , Kayhan Azadmanesh ,...

Efficient and versatile CRISPR engineering of human neurons in culture to model neurological disorders.

The recent identification of multiple new genetic causes of neurological disorders highlights the need for model systems that give experimental access to the underlying biology. In particular, the ability to couple disease-causing mutations with...

Authors: Shah RR1, Cholewa-Waclaw J1, Davies FCJ2, Paton KM1, Chaligne R3, Heard E3, Abbott CM2, Bird AP1.

SST Peer Reviewed

Increased yields and biological potency of knob into-hole-based soluble MHC class II molecules

Assembly of soluble peptide-major histocompatibility complex class II (pMHCII) monomersinto multimeric structures enables the detection of antigen-specific CD4+ T cells in biologicalsamples and, in some configurations, their reprogramming in vivo....

Authors: Pau Serra, Nahir Garabatos, Santiswarup Singha, César Fandos, Josep Garnica, Patricia Solé,Daniel...

CRISPR-Cas9 genome engineering of primary CD4+ T cells for the interrogation of HIV-host factor interactions.

CRISPR-Cas9 gene-editing strategies have revolutionized our ability to engineer the human genome for robust functional interrogation of complex biological processes. We have recently adapted this technology for use in primary human CD4+ T cells to...

Authors: Hultquist JF, Hiatt J, Schumann K, McGregor MJ, Roth TL, Haas P, Doudna JA, Marson A, Krogan NJ.

SST Peer Reviewed

Comparison of commercially available media for hepatic differentiation and hepatocyte maintenance

Human hepatocytes are essential materials in pharmaceutical researches. Not only primary human hepatocytes (PHH) but also human iPS cell-derived hepatocyte-like cells (human iPS-HLCs) are expected to be applied as materials for pharmaceutical...

Authors: Toba Y, Deguchi S, Mimura N, Sakamoto A, Harada K, Hirata K, Takayama K, Mizuguchi H.

High cell density transient transfection of CHO cellsfor TGF-beta1 expression

High cell densities for transient transfection with polyethyleneimine (PEI) can be usedfor rapid and maximal production of recombinant proteins. High cell densities can beobtained by different cultivation systems, such as batch or perfusion systems....

Authors: Abdalla A. Elshereef, André Jochums Antonina Lavrentieva,Lena Stuckenberg, Thomas Scheper Dörte...

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