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Altered COVID-19 Receptor ACE2 Expression in a Higher Risk Group for Cerebrovascular Disease and Ischemic Stroke

Coronavirus disease 2019 (COVID-19) is a worldwide pandemic. It has a high transmission rate among humans, and is a threat to global public health. However, there are no effective prophylactics or therapeutics available. It is necessary to identify...

Authors: Choi JY, Lee HK, Park JH, Cho SJ, Kwon M, Jo C, Koh YH

SST Peer Reviewed

Single-Step, High-Efficiency CRISPR-Cas9 Genome Editing in Primary Human Disease-Derived Fibroblasts.

Genome editing is a tool that has many applications, including the validation of potential drug targets. However, performing genome editing in low-passage primary human cells with the greatest physiological relevance is notoriously difficult. High...

Authors: Martufi M, Good RB, Rapiteanu R, Schmidt T, Patili E, Tvermosegaard K, New M, Nanthakumar CB, Betts...

SST Peer Reviewed

CRISPR/Cas9 Genome Engineering in Engraftable Human Brain-Derived Neural Stem Cells.

Human neural stem cells (NSCs) offer therapeutic potential for neurodegenerative diseases, such as inherited monogenic nervous system disorders, and neural injuries. Gene editing in NSCs (GE-NSCs) could enhance their therapeutic potential. We show...

Authors: Dever DP, Scharenberg SG, Camarena J, Kildebeck EJ, Clark JT, Martin RM, Bak RO, Tang Y, Dohse M,...

Generation of integration-free iPS cell lines from three sickle cell disease patients from the state of Bahia, Brazil

Sickle cell disease (SCD) is one of the most prevalent and severe monogenetic disorders, affecting several million people around the world. Clinical manifestationsand complications of the disease include sickle cell pain crisis, silent cerebral...

Authors: Martins GLS, Paredes BD, Azevedo CM, Sampaio GLA, Nonaka CKV, Cavalcante BRR, Da Silva KN, Pereira...

SST Peer Reviewed

Brain regulatory T cells suppress astrogliosis and potentiate neurological recovery.

In addition to maintaining immune tolerance, FOXP3+ regulatory T (Treg) cells perform specialized functions in tissue homeostasis and remodelling1,2. However, the characteristics and functions of brain Treg cells are not well understood because there...

Authors: Ito M, Komai K, Mise-Omata S, Iizuka-Koga M, Noguchi Y, Kondo T, Sakai R, Matsuo K, Nakayama T,...

Engineering of CRISPR-Cas12b for human genome editing.

The type-V CRISPR effector Cas12b (formerly known as C2c1) has been challenging to develop for genome editing in human cells, at least in part due to the high temperature requirement of the characterized family members. Here we explore the diversity...

Authors: Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F.

SST Peer Reviewed

Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies

Sickle cell disease (SCD) is one of the most prevalent and severe monogenetic disorders. Previously, we generated iPS cell lines from SCD patients. Here, we generatediPS cell lines from three age-, ethnicity- and gender-matched healthy individuals as...

Authors: Paredes BD, Martins GLS, Azevedo CM, Sampaio GLA, Nonaka CKV, Silva KND, Soares MBP, Santos...

SST Peer Reviewed

Identification of a Potential Biomarker for FABP4Inhibition: The Power of Lipidomics in Preclinical Drug Testing

The fatty acid binding protein 4 (FABP4) belongs to the family of lipid chaperones that control intracellular fluxes and compartmentalizationof their respective ligands (e.g., fatty acids). FABP4, which is almost exclusively expressed in...

Authors: Karsten Suhre, Werner Römisch-Margl, Martin Hrabé de Angelis,Jerzy Adamski, Gerd Luippold, and...

IL-7 receptor influences anti-TNFresponsiveness and T cell gut homing ininflammatory bowel disease

It remains unknown what causes inflammatory bowel disease (IBD), including signaling networks perpetuating chronicgastrointestinal inflammation in Crohn’s disease (CD) and ulcerative colitis (UC), in humans. According to an analysis of up to500...

Authors: Lyssia Belarif, Bernard Vanhove, Nicolas Poirier

Gene correction for SCID-X1 in long-term hematopoietic stem cells.

Gene correction in human long-term hematopoietic stem cells (LT-HSCs) could be an effective therapy for monogenic diseases of the blood and immune system. Here we describe an approach for X-linked sSevere cCombined iImmunodeficiency (SCID-X1) using...

Authors: Pavel-Dinu M, Wiebking V, Dejene BT, Srifa W, Mantri S, Nicolas CE, Lee C, Bao G, Kildebeck EJ,...

SST Peer Reviewed

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Altered COVID-19 Receptor ACE2 Expression in a Higher Risk Group for Cerebrovascular Disease and Ischemic Stroke

Single-Step, High-Efficiency CRISPR-Cas9 Genome Editing in Primary Human Disease-Derived Fibroblasts.

CRISPR/Cas9 Genome Engineering in Engraftable Human Brain-Derived Neural Stem Cells.

Generation of integration-free iPS cell lines from three sickle cell disease patients from the state of Bahia, Brazil

Brain regulatory T cells suppress astrogliosis and potentiate neurological recovery.

Engineering of CRISPR-Cas12b for human genome editing.

Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies

Identification of a Potential Biomarker for FABP4Inhibition: The Power of Lipidomics in Preclinical Drug Testing

IL-7 receptor influences anti-TNFresponsiveness and T cell gut homing ininflammatory bowel disease

Gene correction for SCID-X1 in long-term hematopoietic stem cells.