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Cytokines induced killer cells produced in good manufacturing practices conditions: identification of the most advantageous and safest expansion method in terms of viability, cellular growth and identity.

Cytokine-induced killer (CIK) cells are a very promising cell population raising growing interest in the field of cellular antitumor therapy. The aim of our study was to validate the most advantageous expansion method for this advanced therapy...

Authors: Castiglia S, Adamini A, Rustichelli D, Castello L, Mareschi K, Pinnetta G, Leone M, Mandese A,...

SST Peer Reviewed

NK cells switch from granzyme B to death receptor-mediated cytotoxicity during serial killing.

NK cells eliminate virus-infected and tumor cells by releasing cytotoxic granules containing granzyme B (GrzB) or by engaging death receptors that initiate caspase cascades. The orchestrated interplay between both cell death pathways remains poorly...

Authors: Prager I, Liesche C, van Ooijen H, Urlaub D, Verron Q, Sandström N, Fasbender F, Claus M, Eils R,...

The CRISPR/Cas9 system sheds new lights on the biology of protozoan parasites.

The CRISPR/Cas9 system, a natural defence system of bacterial organisms, has recently been used to modify genomes of the most important protozoa parasites. Successful genome manipulations with...

Authors: Grzybek M1, Golonko A2, Górska A3, Szczepaniak K3, Strachecka A4, Lass A5, Lisowski P6,7.

SST Peer Reviewed

Deactivation of Hepatic Stellate Cells by Culturing on VECELL Inserts

Hepatic stellate cells play a cardinal role in the development of liver fibrosis. Quiescent hepatic stellate cells isolated from normal liver are activated by plating on a plastic culture dish. Therefore, a culture method that maintains hepatic...

Authors: Shinichiro Horiuchi, Yukie Kuroda, Ryuya Fujii, Su-ryang Kim, Seiichi Ishida

SST Peer Reviewed

Functionality of Primary Hepatic Non-Parenchymal Cells in a 3D Spheroid Model and Contribution to Acetaminophen Hepatotoxicity

In addition to hepatocytes, the liver comprises a host of specialised non-parenchymal cells which are important to consider in the development of in vitro models which are both physiologically and toxicologically relevant. We have characterized...

Authors: Catherine C Bell , Bhavik Chouhan , Linda C Andersson , Håkan Andersson , James W Dear , Dominic P...

SST Peer Reviewed

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms.

Site-specific eukaryotic genome editing with CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) systems has quickly become a commonplace amongst researchers pursuing a wide variety of biological questions....

Authors: Farboud B1, Jarvis E2, Roth TL3, Shin J4, Corn JE4, Marson A5, Meyer BJ1, Patel NH6, Hochstrasser...

SST Peer Reviewed

Stem cell-derived polarized hepatocytes

Human stem cell-derived hepatocyte-like cells (HLCs) offer an attractive platform to study liver biology. Despite their numerous advantages, HLCs lack critical in vivo characteristics, including cell polarity. Here, we report a stem cell...

Authors: Viet Loan Dao Thi, Xianfang Wu, Rachel L. Belote, Ursula Andreo, Constantin N. Takacs, Joseph P....

In vitro analysis of arrhythmogenic cardiomyopathy associated desmoglein-2 (DSG2) mutations reveals diverse glycosylation patterns.

Arrhythmogenic right ventricular cardiomyopathy is a heritable cardiac disease causing severe ventricular arrhythmias, heart failure and sudden cardiac death. It is mainly caused by mutations in genes encoding several structural proteins of the...

Authors: Debus JD, Milting H, Brodehl A, SaveKassner A, Anselmetti D, Gummert J, Gaertner-Rommel A.

Distinct Mitochondrial Defects Trigger the Integrated Stress Response Depending on the Metabolic State of the Cell

Mitochondrial dysfunction is associated with activation of the integrated stress response (ISR) but the underlying triggers remain unclear. We systematically combined acute mitochondrial inhibitors with genetic tools for compartment-specific NADH...

Authors: Eran Mick, Denis V Titov, Owen S Skinner, Rohit Sharma, Alexis A Jourdain, Vamsi K Mootha

CRISPR-Mediated Editing of the B Cell Receptor in Primary Human B Cells.

Vaccination approaches have generally focused on the antigen rather than the resultant antibodies generated, which differ greatly in quality and function between individuals. The ability to replace the variable regions of the native B cell receptor...

Authors: Greiner V, Bou Puerto R, Liu S, Herbel C, Carmona EM, Goldberg MS.

SST Peer Reviewed

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Cytokines induced killer cells produced in good manufacturing practices conditions: identification of the most advantageous and safest expansion method in terms of viability, cellular growth and identity.

NK cells switch from granzyme B to death receptor-mediated cytotoxicity during serial killing.

The CRISPR/Cas9 system sheds new lights on the biology of protozoan parasites.

Deactivation of Hepatic Stellate Cells by Culturing on VECELL Inserts

Functionality of Primary Hepatic Non-Parenchymal Cells in a 3D Spheroid Model and Contribution to Acetaminophen Hepatotoxicity

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms.

Stem cell-derived polarized hepatocytes

In vitro analysis of arrhythmogenic cardiomyopathy associated desmoglein-2 (DSG2) mutations reveals diverse glycosylation patterns.

Distinct Mitochondrial Defects Trigger the Integrated Stress Response Depending on the Metabolic State of the Cell

CRISPR-Mediated Editing of the B Cell Receptor in Primary Human B Cells.