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Protein O-fucosyltransferase 2-mediated O-glycosylation of the adhesin MIC2 is dispensable for Toxoplasma gondii tachyzoite infection.

Toxoplasma gondii is a ubiquitous, obligate intracellular eukaryotic parasite that causes congenital birth defects, disease in immunocompromised individuals, and blindness. Protein glycosylation plays an important role in the infectivity and evasion...

Authors: Khurana S, Coffey MJ, John A, Uboldi AD, Huynh MH, Stewart RJ, Carruthers V, Tonkin CJ,...

SST Peer Reviewed

Protein Kinase A Is Essential for Invasion of Plasmodium falciparum into Human Erythrocytes.

Understanding the mechanisms behind host cell invasion by Plasmodium falciparum remains a major hurdle to developing antimalarial therapeutics that target the asexual cycle and the symptomatic stage of malaria. Host cell entry is enabled by...

Authors: Wilde ML1,2, Triglia T1, Marapana D1, Thompson JK1, Kouzmitchev AA3, Bullen HE4, Gilson...

SST Peer Reviewed

Highly efficient genome editing in primary bronchial epithelial cells establishes FOXJ1 as essential for ciliation in human airways

The structure and composition of the bronchial epithelium is altered in respiratory diseases such as COPD and asthma, in which goblet cell hyperplasia and reduced numbers of ciliated cells impair mucociliary clearance. We describe a robust genome...

Authors: Radu Rapiteanu??1, Tina Karagyozova1, Natalie Zimmermann2, Gareth Wayne1, MatteoMartufi1, Nikolai N...

SST Peer Reviewed

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins.

CRISPR/Cas9 technology is accelerating genome engineering in many cell types, but so far, gene delivery and stable gene modification have been challenging in primary NK cells. For example, transgene delivery using lentiviral or retroviral...

Authors: Naeimi Kararoudi, M., Dolatshad, H., Trikha, P., Hussain, S. R., Elmas, E., Foltz, J. A., Moseman,...

SST Peer Reviewed

Deletion of Plasmodium falciparum Protein RON3 Affects the Functional Translocation of Exported Proteins and Glucose Uptake.

The survival of Plasmodium spp. within the host red blood cell (RBC) depends on the function of a membrane protein complex, termed the Plasmodium translocon of exported proteins (PTEX), that exports certain...

Authors: Low LM#1, Azasi Y#1, Sherling ES#1,2, Garten M3, Zimmerberg J3, Tsuboi T4, Brzostowski J5, Mu...

SST Peer Reviewed

Efficient gene knockout in primary human and murine myeloid cells by non-viral delivery of CRISPR-Cas9.

Myeloid cells play critical and diverse roles in mammalian physiology, including tissue development and repair, innate defense against pathogens, and generation of adaptive immunity. As cells that show prolonged recruitment to sites of injury or...

Authors: Freund EC, Lock JY, Oh J, Maculins T, Delamarre L, Bohlen CJ, Haley B, Murthy A.

SST Peer Reviewed

Bacteria-free minicircle DNA system to generate integration-free CAR-T cells

BACKGROUND:Chimeric antigen receptor T (CAR-T) cells engineered with lentiviral and retroviral vectors have been successfully applied to treat patients with B cell malignancy. However, viral integration in T cells has the potential risk of...

Authors: Chen Cheng, Na Tang, Jiaxin Li, Shiwei Cao, Tongtong Zhang, Xiaofei Wei, Haoyi Wang

SST Peer Reviewed

A Plasmodium falciparum C-mannosyltransferase is dispensable for parasite asexual blood stage development.

C-mannosylation was recently identified in the thrombospondin-related anonymous protein (TRAP) from Plasmodium falciparum salivary gland sporozoites. A candidate P. falciparum C-mannosyltransferase (PfDPY-19) was demonstrated...

Authors: López-Gutiérrez B1, Cova M1, Izquierdo L1.

SST Peer Reviewed

Live cell imaging of single genomic loci with quantum dot-labeled TALEs

Single genomic loci are often related to specific cellular functions, genetic diseases, or pathogenic infections. Visualization of single genomic loci in live human cells is currently of great interest, yet it remains challenging. Here, we describe a...

Authors: Yingxin Ma, Mingxiu Wang, Wei Li, Zhiping Zhang, Xiaowei Zhang, Tianwei Tan, Xian-En Zhang,...

SST Peer Reviewed

Rapid and iterative genome editing in the malaria parasite Plasmodium knowlesi provides new tools for P. vivax research.

Tackling relapsing Plasmodium vivax and zoonotic Plasmodium knowlesi infections is critical to reducing malaria incidence and mortality worldwide. Understanding the biology of these important and related...

Authors: Mohring F1, Hart MN1, Rawlinson TA2, Henrici R1, Charleston JA1, Diez Benavente E1, Patel A1, Hall...

SST Peer Reviewed

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Protein O-fucosyltransferase 2-mediated O-glycosylation of the adhesin MIC2 is dispensable for Toxoplasma gondii tachyzoite infection.

Protein Kinase A Is Essential for Invasion of Plasmodium falciparum into Human Erythrocytes.

Highly efficient genome editing in primary bronchial epithelial cells establishes FOXJ1 as essential for ciliation in human airways

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins.

Deletion of Plasmodium falciparum Protein RON3 Affects the Functional Translocation of Exported Proteins and Glucose Uptake.

Efficient gene knockout in primary human and murine myeloid cells by non-viral delivery of CRISPR-Cas9.

Bacteria-free minicircle DNA system to generate integration-free CAR-T cells

A Plasmodium falciparum C-mannosyltransferase is dispensable for parasite asexual blood stage development.

Live cell imaging of single genomic loci with quantum dot-labeled TALEs

Rapid and iterative genome editing in the malaria parasite Plasmodium knowlesi provides new tools for P. vivax research.