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Enhanced transfection of cell lines from Atlantic salmon through nucoleofection and antibiotic selection.

BACKGROUND:Cell lines from Atlantic salmon kidney have made it possible to culture and study infectious salmon anemia virus (ISAV), an aquatic orthomyxovirus affecting farmed Atlantic salmon. However, transfection of these cells using calcium...

Authors: Schiøtz BL1, Rosado EG, Baekkevold ES, Lukacs M, Mjaaland S, Sindre H, Grimholt U, Gjøen T.

SST Peer Reviewed

Flow Cytometry Based Detection and Isolation of Plasmodium falciparum Liver Stages In Vitro

Malaria, the disease caused by Plasmodium parasites, remains a major global health burden. The liver stage of Plasmodium falciparuminfection is a leading target for immunological and pharmacological interventions....

Authors: Dumoulin PC, Trop SA, Ma J, Zhang H, Sherman MA, Levitskaya J

Olinciguat, an Oral sGC Stimulator, Exhibits Diverse Pharmacology Across Preclinical Models of Cardiovascular, Metabolic, Renal, and Inflammatory Disease

Nitric oxide (NO)-soluble guanylate cyclase (sGC)-cyclic 3',5' GMP (cGMP) signaling plays a central role in regulation of diverse processes including smooth muscle relaxation, inflammation, and fibrosis. sGC is activated by the...

Authors: Zimmer DP, Shea CM, Tobin JV, Tchernychev B, Germano P, Sykes K, Banijamali AR, Jacobson S, Bernier...

Optimization of Normal Human Bronchial Epithelial (NHBE) Cell 3D Cultures for in vitro Lung Model Studies.

Robust in vitro lung models are required for risk assessment to measure key events leading to respiratory diseases. Primary normal human bronchial epithelial cells (NHBE) represent a good lung model but obtaining well-differentiated 3D cultures can...

Authors: Rayner RE, Makena P, Prasad GL, Cormet-Boyaka E.

SST Peer Reviewed

Interference with ERK-dimerization at thenucleocytosolic interface targets pathologicalERK1/2 signaling without cardiotoxic side-effects

Dysregulation of extracellular signal-regulated kinases (ERK1/2) is linked to several diseases including heart failure, genetic syndromes and cancer. Inhibition of ERK1/2, however, can cause severe cardiac side-effects, precluding its wide...

Authors: Tomasovic A, Brand T, Schanbacher C, Kramer S, Hümmert MW, Godoy P, Schmidt-Heck W, Nordbeck...

Search-and-replace genome editing without double-strand breaks or donor DNA.

Most genetic variants that contribute to disease1 are challenging to correct efficiently and without excess byproducts2-5. Here we describe prime editing, a versatile and precise genome editing method that directly writes new genetic information into...

Authors: Anzalone AV1,2,3, Randolph PB1,2,3, Davis JR1,2,3, Sousa AA1,2,3, Koblan LW1,2,3, Levy JM1,2,3, Chen...

SST Peer Reviewed

Changes in the function of angiotensin II type 1 receptor due to cholesterol depletion from cell membrane

Blockers of G-protein coupled receptors (GPCRs), angiotensin II (Ang II) type 1 (AT1) receptor and ß1-adrenergic (Ad) receptor, have been shown to improve the prognosis of cardiovascular disease. Cholesterol molecules in the cell membrane are needed...

Authors: Matsuo Y, Suematsu Y, Idemoto Y, Kuwano T, Kitajima K, Miura SI

Cancer invasion regulates vascular complexity in a three-dimensional biomimetic model.

INTRODUCTION:There is a growing appreciation for including a complex, vascularised stroma in three-dimensional (3D) tumour models to recapitulate the native tumour microenvironment in situ.METHODS:Using a compartmentalised, biomimetic, 3D cancer...

Authors: Pape J1, Magdeldin T1, Ali M2, Walsh C2, Lythgoe M2, Emberton M3, Cheema U4.

SST Peer Reviewed

A 3D Heterotypic Multicellular Tumor Spheroid Assay Platform to Discriminate Drug Effects on Stroma versus Cancer Cells.

Three-dimensional (3D) cell culture models are thought to mimic the physiological and pharmacological properties of tissues in vivo more accurately than two-dimensional cultures on plastic dishes. For the development of cancer therapies, 3D spheroid...

Authors: Weydert Z, Lal-Nag M, Mathews-Greiner L, Thiel C, Cordes H, Küpfer L, Guye P, Kelm JM, Ferrer M.

SST Peer Reviewed

Imbalanced host response to SARS-CoV-2 drives development of COVID-19

Viral pandemics, such as the one caused by SARS-CoV-2, pose an imminent threat to humanity. Because of its recent emergence, there is a paucity of information regarding viral behavior and host response following SARS-CoV-2 infection. Here, we offer...

Authors: Daniel Blanco-Melo, Benjamin E. Nilsson-Payant, Wen-Chun Liu, Skyler Uhl, Daisy Hoagland, Rasmus...

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Enhanced transfection of cell lines from Atlantic salmon through nucoleofection and antibiotic selection.

Flow Cytometry Based Detection and Isolation of Plasmodium falciparum Liver Stages In Vitro

Olinciguat, an Oral sGC Stimulator, Exhibits Diverse Pharmacology Across Preclinical Models of Cardiovascular, Metabolic, Renal, and Inflammatory Disease

Optimization of Normal Human Bronchial Epithelial (NHBE) Cell 3D Cultures for in vitro Lung Model Studies.

Interference with ERK-dimerization at thenucleocytosolic interface targets pathologicalERK1/2 signaling without cardiotoxic side-effects

Search-and-replace genome editing without double-strand breaks or donor DNA.

Changes in the function of angiotensin II type 1 receptor due to cholesterol depletion from cell membrane

Cancer invasion regulates vascular complexity in a three-dimensional biomimetic model.

A 3D Heterotypic Multicellular Tumor Spheroid Assay Platform to Discriminate Drug Effects on Stroma versus Cancer Cells.

Imbalanced host response to SARS-CoV-2 drives development of COVID-19