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How can I find out if other 24-well culture plates than Greiner or Nunc are compatible to the dipping electrode array for adherent Nucleofection® using the 4D-Nucleofector® Y Unit?

Please find attached a list with all plate types that have been checked for compatibility so far. If your plate type is not on this list, please contact our Scientific Support Team we can investigate compatibility.
Missing: mycoalert

The basic protocol for adherent Nucleofection« of neurons using the 4D-Nucleofector® Y Unit recommends to plate 150,000 freshly isolated or 300,000 cryopreserved neurons. What can I do if do not have enough cells to perform several samples?

...for the initial optimization experiment we would recommend to plate about 40,000 - 60,000 cells onto a 48-well coverslip and place this into the 24-well plate for Nucleofection®....
Missing: mycoalert

How can I detect mycoplasma contamination of my cells?

We recommend using the 20-minute MycoAlert® Mycoplasma Detection Kit from Lonza. The simple four-step MycoAlert® Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes. The presence of these enzymes provides a rapid...
Missing: plate

Do all the luciferase based reagents in the Lonza BioAssays need to be protected from light?

Yes, all of our reagents include in ToxiLight™ Non-Destructive Cytotoxicity BioAssay, the ViaLight™ Plus BioAssay, the MycoAlert™ and MycoAlert™ Plus Assay, the PPiLight™ Inorganic Pyrophosphate Assay and the PDELight™ HTS cAMP...
Missing: plate

How can cells be cured of mycoplasma contamination?

...Mollicute sp., including all species of mycoplasma, acholeplasma, spiroplasma and entomoplasma in cell cultures. Cultures should be tested with the MycoAlert® or MycoAlert® Plus Mycoplasma Detection Kit at regular intervals for 4-6 weeks after mycoplasma...
Missing: plate

How can cells be cured of mycoplasma contamination?

...Mollicute sp., including all species of mycoplasma, acholeplasma, spiroplasma and entomoplasma in cell cultures. Cultures should be tested with the MycoAlert® Mycoplasma Detection Kit at regular intervals for 4-6 weeks after mycoplasma elimination to ensure...
Missing: plate

I would like to use the dipping electrode array for less than 24 wells. How can I keep non-required dipping electrodes clean?

We would recommend to prepare the culture plate as such that cells are only plated into those wells that should be transfected and leave further wells empty.After withdrawing the dipping electrode array from the plate you can wipe off residual...
Missing: mycoalert

Your instructions for culturing of Clonetics™ Cells do not mention centrifuging the cells upon thawing. Won't the DMSO kill the cells?

...of centrifuging the cells, we recommend plating the cells directly at our recommended plating density for that cell type. When plated at this density, the residual DMSO will not be sufficient to affect the cells and the cells will not be harmed by the effects...
Missing: mycoalert

How can I best clean used dipping electrodes and store unused for later use? Can I wash used dipping electrodes in an ethanol bath?

After withdrawing the dipping electrode array from the plate you can wipe off residual liquid with a tissue. Alternatively, you can wash used electrodes with ethanol which won't damage the electrodes. To avoid cross-contamination of non-used...
Missing: mycoalert

How can I improve Human Hepatocyte viability post Nucleofection™ on the 96-well Shuttle™ device?

...in the Nucleocuvette™ plate after Nucleofection. This can be done by adding to each well, 180ul Ficoll solution (25% Ficoll in Plating medium Lonza Basal Medium (Cat.No.:CC-3198) supplemented with BulletKit) and centrifuging the cells in the Nucleocuvette for 4...
Missing: mycoalert
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