Search Results

Deactivation of Hepatic Stellate Cells by Culturing on VECELL Inserts

Hepatic stellate cells play a cardinal role in the development of liver fibrosis. Quiescent hepatic stellate cells isolated from normal liver are activated by plating on a plastic culture dish. Therefore, a culture method that maintains hepatic...

Authors: Shinichiro Horiuchi, Yukie Kuroda, Ryuya Fujii, Su-ryang Kim, Seiichi Ishida

SST Peer Reviewed

Expansion of bone marrow-derived human mesenchymal stem/stromal cells (hMSCs) using a two-phase liquid/liquid system.

BACKGROUND:Human mesenchymal stem/stromal cells (hMSCs) are at the forefront of regenerative medicine applications due to their relatively easy isolation and availability in adults, potential to differentiate and to secrete a range of trophic factors...

Authors: Hanga MP1,2, Murasiewicz H3,4, Pacek AW3, Nienow AW1,3,2, Coopman K1, Hewitt CJ1,2.

Autologous and Heterologous Cell Therapy for Hemophilia B toward Functional Restoration of Factor IX.

Hemophilia B is an ideal target for gene- and cell-based therapies because of its monogenic nature and broad therapeutic index. Here, we demonstrate the use of cell therapy as a potential long-term cure for hemophilia B in our FIX-deficient mouse...

Authors: Ramaswamy S, Tonnu N, Menon T, Lewis BM, Green KT, Wampler D, Monahan PE, Verma IM.

Quantification of focal adhesion dynamics of cell movement based on cell-induced collagen matrix deformation using second-harmonic generation microscopy

Mechanical interactions of living cells with the surrounding environment via focal adhesion (FA) in three dimensions (3-D) play a key role in dynamic biological events, such as tissue regeneration, wound healing, and cancer invasion. Recently,...

Authors: Kang YG1, Jang H2, Yang TD1, Notbohm J3, Choi Y1, Park Y2, Kim BM1

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms.

Site-specific eukaryotic genome editing with CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) systems has quickly become a commonplace amongst researchers pursuing a wide variety of biological questions....

Authors: Farboud B1, Jarvis E2, Roth TL3, Shin J4, Corn JE4, Marson A5, Meyer BJ1, Patel NH6, Hochstrasser...

SST Peer Reviewed

The Effect of Antidepressants on Mesenchymal Stem Cell Differentiation

Background:Use of antidepressant medications has been linked to detrimental impacts on bone mineral density and osteoporosis; however, the cellular basis behind these observations remains poorly understood. The effect does not appear to be...

Authors: Kruk JS, Bermeo S, Skarratt KK, Fuller SJ, Duque G.

A Plasmodium falciparum C-mannosyltransferase is dispensable for parasite asexual blood stage development.

C-mannosylation was recently identified in the thrombospondin-related anonymous protein (TRAP) from Plasmodium falciparum salivary gland sporozoites. A candidate P. falciparum C-mannosyltransferase (PfDPY-19) was demonstrated...

Authors: López-Gutiérrez B1, Cova M1, Izquierdo L1.

SST Peer Reviewed

In vitro analysis of arrhythmogenic cardiomyopathy associated desmoglein-2 (DSG2) mutations reveals diverse glycosylation patterns.

Arrhythmogenic right ventricular cardiomyopathy is a heritable cardiac disease causing severe ventricular arrhythmias, heart failure and sudden cardiac death. It is mainly caused by mutations in genes encoding several structural proteins of the...

Authors: Debus JD, Milting H, Brodehl A, SaveKassner A, Anselmetti D, Gummert J, Gaertner-Rommel A.

Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis.

BACKGROUND:In this study, we examined the effect of oxidative stress on cellular energy metabolism and pro-angiogenic/pro-inflammatory mechanisms of primary rheumatoid arthritis synovial fibroblast cells (RASFC) and human umbilical vein endothelial...

Authors: Balogh E, Veale DJ, McGarry T, Orr C, Szekanecz Z, Ng CT, Fearon U, Biniecka M

Rapid and iterative genome editing in the malaria parasite Plasmodium knowlesi provides new tools for P. vivax research.

Tackling relapsing Plasmodium vivax and zoonotic Plasmodium knowlesi infections is critical to reducing malaria incidence and mortality worldwide. Understanding the biology of these important and related...

Authors: Mohring F1, Hart MN1, Rawlinson TA2, Henrici R1, Charleston JA1, Diez Benavente E1, Patel A1, Hall...

SST Peer Reviewed

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Category

Research Area

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Topic

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Characteristic

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Origin

Deactivation of Hepatic Stellate Cells by Culturing on VECELL Inserts

Expansion of bone marrow-derived human mesenchymal stem/stromal cells (hMSCs) using a two-phase liquid/liquid system.

Autologous and Heterologous Cell Therapy for Hemophilia B toward Functional Restoration of Factor IX.

Quantification of focal adhesion dynamics of cell movement based on cell-induced collagen matrix deformation using second-harmonic generation microscopy

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms.

The Effect of Antidepressants on Mesenchymal Stem Cell Differentiation

A Plasmodium falciparum C-mannosyltransferase is dispensable for parasite asexual blood stage development.

In vitro analysis of arrhythmogenic cardiomyopathy associated desmoglein-2 (DSG2) mutations reveals diverse glycosylation patterns.

Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis.

Rapid and iterative genome editing in the malaria parasite Plasmodium knowlesi provides new tools for P. vivax research.