Search Results

Bacteria-free minicircle DNA system to generate integration-free CAR-T cells

BACKGROUND:Chimeric antigen receptor T (CAR-T) cells engineered with lentiviral and retroviral vectors have been successfully applied to treat patients with B cell malignancy. However, viral integration in T cells has the potential risk of...

Authors: Chen Cheng, Na Tang, Jiaxin Li, Shiwei Cao, Tongtong Zhang, Xiaofei Wei, Haoyi Wang

SST Peer Reviewed

Live cell imaging of single genomic loci with quantum dot-labeled TALEs

Single genomic loci are often related to specific cellular functions, genetic diseases, or pathogenic infections. Visualization of single genomic loci in live human cells is currently of great interest, yet it remains challenging. Here, we describe a...

Authors: Yingxin Ma, Mingxiu Wang, Wei Li, Zhiping Zhang, Xiaowei Zhang, Tianwei Tan, Xian-En Zhang,...

SST Peer Reviewed

In Vitro Experimental Model for the Long-Term Analysis of Cellular Dynamics During Bronchial Tree Development from Lung Epithelial Cells.

Lung branching morphogenesis has been studied for decades, but the underlying developmental mechanisms are still not fully understood. Cellular movements dynamically change during the branching process, but it is difficult to observe long-term...

Authors: Hagiwara M1, Maruta N2,3, Marumoto M2

Cytokines induced killer cells produced in good manufacturing practices conditions: identification of the most advantageous and safest expansion method in terms of viability, cellular growth and identity.

Cytokine-induced killer (CIK) cells are a very promising cell population raising growing interest in the field of cellular antitumor therapy. The aim of our study was to validate the most advantageous expansion method for this advanced therapy...

Authors: Castiglia S, Adamini A, Rustichelli D, Castello L, Mareschi K, Pinnetta G, Leone M, Mandese A,...

SST Peer Reviewed

Induction of expression of aryl hydrocarbon receptor-dependent genes in human HepaRG cell line modified by shRNA and treated with ß-naphthoflavone

The aryl hydrocarbon receptor (AhR) mediates a variety of biological responses to ubiquitous environmental pollutants. In this study, the effects of administration of ß-naphthoflavone (BNF), a potent AhR ligand, on the expression of AhR-dependent...

Authors: Brauze D1, Zawierucha P2,3, Kiwerska K4, Bednarek K4, Oleszak M4, Rydzanicz M4,5, Jarmuz-Szymczak...

Deactivation of Hepatic Stellate Cells by Culturing on VECELL Inserts

Hepatic stellate cells play a cardinal role in the development of liver fibrosis. Quiescent hepatic stellate cells isolated from normal liver are activated by plating on a plastic culture dish. Therefore, a culture method that maintains hepatic...

Authors: Shinichiro Horiuchi, Yukie Kuroda, Ryuya Fujii, Su-ryang Kim, Seiichi Ishida

SST Peer Reviewed

ß2-Adrenoceptor signaling in airway epithelial cells promotes eosinophilic inflammation, mucous metaplasia, and airway contractility

The mostly widely used bronchodilators in asthma therapy are ß2-adrenoreceptor (ß2AR) agonists, but their chronic use causes paradoxical adverse effects. We have previously determined that ß2AR activation is required for expression of the asthma...

Authors: Nguyen LP, Al-Sawalha NA, Parra S, Pokkunuri I, Omoluabi O, Okulate AA, Windham Li E, Hazen M,...

Cellular and Molecular Effects of Prolonged Low-Level Sodium Arsenite Exposure on Human Hepatic HepaRG Cells

Inorganic arsenic is a human carcinogen associated with several types of cancers, including liver cancer. Inorganic arsenic has been postulated to target stem cells, causing their oncogenic transformation. This is proposed to be one of the key events...

Authors: Dreval K1, Tryndyak V1, Kindrat I1,2, Twaddle NC1, Orisakwe OE1,3, Mudalige TK4, Beland FA1, Doerge...

MicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells

While all forms of tobacco exposure have negative health effects, the significance of exposure to electronic cigarettes (eCig) is not fully understood. Here, we studied the global effects of eCig on the micro RNA (miRNA) transcriptome in human lung...

Authors: Solleti SK, Bhattacharya S, Ahmad A, Wang Q, Mereness J, Rangasamy T, Mariani TJ

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms.

Site-specific eukaryotic genome editing with CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) systems has quickly become a commonplace amongst researchers pursuing a wide variety of biological questions....

Authors: Farboud B1, Jarvis E2, Roth TL3, Shin J4, Corn JE4, Marson A5, Meyer BJ1, Patel NH6, Hochstrasser...

SST Peer Reviewed

Data Type

Category

Research Area

Platform

Topic

Species

Characteristic

Format

Origin

Bacteria-free minicircle DNA system to generate integration-free CAR-T cells

Live cell imaging of single genomic loci with quantum dot-labeled TALEs

In Vitro Experimental Model for the Long-Term Analysis of Cellular Dynamics During Bronchial Tree Development from Lung Epithelial Cells.

Cytokines induced killer cells produced in good manufacturing practices conditions: identification of the most advantageous and safest expansion method in terms of viability, cellular growth and identity.

Induction of expression of aryl hydrocarbon receptor-dependent genes in human HepaRG cell line modified by shRNA and treated with ß-naphthoflavone

Deactivation of Hepatic Stellate Cells by Culturing on VECELL Inserts

ß2-Adrenoceptor signaling in airway epithelial cells promotes eosinophilic inflammation, mucous metaplasia, and airway contractility

Cellular and Molecular Effects of Prolonged Low-Level Sodium Arsenite Exposure on Human Hepatic HepaRG Cells

MicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms.