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Switch in Laminin ß2 to Laminin ß1 Isoforms During Aging Controls Endothelial Cell Functions-Brief Report

OBJECTIVE:Endothelial cells play important roles in tissue homeostasis and vascularization, a function that is impaired by aging. Here, we aim to decipher the role of the microenvironment underlying the impairment of endothelial cell functions by...

Authors: Wagner JUG1,2, Chavakis E3, Rogg EM1, Muhly-Reinholz M1, Glaser SF1,2, Günther S4, John D1, Bonini...

A biomimetic hydrogel functionalized with adipose ECM components as a microenvironment for the 3D culture of human and murine adipocytes.

The lack of relevant in vitro models for adipose tissue makes necessary the development of a more physiological environment providing spatial and chemical cues for the effective maturation of adipocytes. We developed a biofunctionalized hydrogel with...

Authors: Louis F, Pannetier P, Souguir Z, Le Cerf D, Valet P, Vannier JP, Vidal G, Demange E

Regeneration of diaphragm with bio-3D cellular patch

Neonates with congenital diaphragmatic hernia often require surgical defect closure with a patch. Alternatives to native diaphragmatic tissue are critically needed for this paediatric surgery. The clinical efficacy of mesh patches is limited by...

Authors: Zhang XY1, Yanagi Y2, Sheng Z3, Nagata K2, Nakayama K4, Taguchi T2

Ribonucleoprotein Transfection for CRISPR/Cas9-Mediated Gene Knockout in Primary T Cells.

CRISPR/Cas9 has enabled the rapid and efficient generation of gene knockouts across various cell types of several species. T cells are central players in adaptive immune responses. Gene editing in primary T cells not only represents a valuable...

Authors: Oh SA, Seki A, Rutz S.

SST Peer Reviewed

Novel Bioengineered Three-Dimensional Human Intestinal Model for Long-Term Infection of Cryptosporidium parvum

Cryptosporidium spp. are apicomplexan parasites of global importance that cause human diarrheal disease. In vitro culture models that may be used to study this parasite and that have physiological relevance to in vivo infection remain suboptimal....

Authors: DeCicco RePass MA1,2, Chen Y3, Lin Y3, Zhou W3, Kaplan DL1,3, Ward HD4,2

Migration of breast cancer cell lines in response to pulmonary laminin 332.

Because tumor cell motility is a requirement for metastasis, we hypothesized that lung tissue harbors substances that induce tumor cell migration. MCF-7 breast carcinoma cells exposed to small airway epithelial cells and conditioned medium exhibited...

Authors: Carpenter PM, Sivadas P, Hua SS, Xiao C, Gutierrez AB, Ngo T, Gershon PD

Activating prostaglandin E2 receptor subtype EP4 increases secreted mucin from airway goblet cells.

Prostaglandin E2 (PGE2) is a ligand of the E-type prostanoid receptors, EP1-4. PGE2 secretion is increased in the airways of patients with asthma by secretory phospholipases A2, which also increases MUC5AC mucin in goblet cells. We hypothesized that...

Authors: Akaba T, Komiya K, Suzaki I, Kozaki Y, Tamaoki J, Rubin BK

Generating conditional gene knockouts in Plasmodium - a toolkit to produce stable DiCre recombinase-expressing parasite lines using CRISPR/Cas9

Successful establishment of CRISPR/Cas9 genome editing technology in Plasmodium spp. has provided a powerful tool to transform Plasmodium falciparum into a genetically more tractable organism. Conditional gene regulation approaches are required to...

Authors: Knuepfer E, Napiorkowska M, van Ooij C, Holder AA

SST Peer Reviewed

Crocin reduces Aspergillus fumigatus-induced airway inflammation and NF-kappaB signal activation

Chronic obstructive pulmonary disease (COPD) is a chronic airway inflammation and its exacerbation is often accompanied by Aspergillus fumigatus (A. fumigatus) infection. Increasing evidences demonstrated the potent antioxidant and -inflammatory...

Authors: Du J1, Chi Y1, Song Z1, Di Q1, Mai Z1, Shi J1, Li M1

CRISPRs for Optimal Targeting: Delivery of CRISPR Components as DNA, RNA, and Protein into Cultured Cells and Single-Cell Embryos.

The rapid development of CRISPR technology greatly impacts the field of genetic engineering. The simplicity in design and generation of highly efficient CRISPR reagents allows more and more researchers to take on genome editing in different model...

Authors: Kouranova E, Forbes K, Zhao G, Warren J, Bartels A, Wu Y, Cui .

SST Peer Reviewed

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Switch in Laminin ß2 to Laminin ß1 Isoforms During Aging Controls Endothelial Cell Functions-Brief Report

A biomimetic hydrogel functionalized with adipose ECM components as a microenvironment for the 3D culture of human and murine adipocytes.

Regeneration of diaphragm with bio-3D cellular patch

Ribonucleoprotein Transfection for CRISPR/Cas9-Mediated Gene Knockout in Primary T Cells.

Novel Bioengineered Three-Dimensional Human Intestinal Model for Long-Term Infection of Cryptosporidium parvum

Migration of breast cancer cell lines in response to pulmonary laminin 332.

Activating prostaglandin E2 receptor subtype EP4 increases secreted mucin from airway goblet cells.

Generating conditional gene knockouts in Plasmodium - a toolkit to produce stable DiCre recombinase-expressing parasite lines using CRISPR/Cas9

Crocin reduces Aspergillus fumigatus-induced airway inflammation and NF-kappaB signal activation

CRISPRs for Optimal Targeting: Delivery of CRISPR Components as DNA, RNA, and Protein into Cultured Cells and Single-Cell Embryos.