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A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions.

BACKGROUND:Adult bone marrow contains stem cells that replenish the myeloid and lymphoid lineages. A subset of human and mouse CD34+ bone marrow stem cells can be propagated in culture to autonomously express embryonic stem cell genes and embryonic...

Authors: Makar T, Nimmagadda VK, Guda PR, Hampton B, Huang W, Kane MA, Fishman PS, Pessac B, Bever CT Jr,...

SST Peer Reviewed

ADME of biologics-what have we learned from small molecules?

Thorough characterization and in-depth understanding of absorption, distribution, metabolism, and elimination (ADME) properties of a drug candidate have been well recognized as an important element in small molecule (SM) drug discovery and...

Authors: Prueksaritanont T1, Tang C.

HIF1a-dependent glycolysis promotes macrophage functional activities in protecting against bacterial and fungal infection

Macrophages are important innate immune defense system cells in the fight against bacterial and fungal pathogenic infections. They exhibit significant plasticity, particularly with their ability to undergo functional differentiation. Additionally,...

Authors: Li C1,2, Wang Y1,2, Li Y1,2, Yu Q2, Jin X2, Wang X1,2, Jia A2, Hu Y2, Han L2, Wang J1,2, Yang H1,...

The utility of the minipig as an animal model in regulatory toxicology

In this article we review the value and utility of the minipig as an animal model in regulatory toxicity testing. Our review is based on detailed consideration of the comparative biology of the minipig, and of the practical features of toxicity...

Authors: Bode G1, Clausing P, Gervais F, Loegsted J, Luft J, Nogues V, Sims J; Steering Group of the RETHINK...

In vitro gene expression analysis of hepatotoxic drugs in rat primary hepatocytes

The study examined the feasibility of screening for hepatotoxicity by an in vitro gene expression analysis using rat primary hepatocytes and Affymetrix Rat Toxicology U34 arrays. Hepatocytes were exposed for 6 or 24 h to eight drugs, with different...

Authors: Suzuki H1, Inoue T, Matsushita T, Kobayashi K, Horii I, Hirabayashi Y, Inoue T.

Evaluation of an in vitro toxicogenetic mouse model for hepatotoxicity

Numerous studies support the fact that a genetically diverse mouse population may be useful as an animal model to understand and predict toxicity in humans. We hypothesized that cultures of hepatocytes obtained from a large panel of inbred mouse...

Authors: Martinez SM1, Bradford BU, Soldatow VY, Kosyk O, Sandot A, Witek R, Kaiser R, Stewart T, Amaral K,...

Guide Swap enables genome-scale pooled CRISPR-Cas9 screening in human primary cells.

CRISPR-Cas9 screening allows genome-wide interrogation of gene function. Currently, to achieve the high and uniform Cas9 expression desirable for screening, one needs to engineer stable and clonal Cas9-expressing cells-an approach that is not...

Authors: Ting PY, Parker AE, Lee JS, Trussell C, Sharif O, Luna F, Federe G, Barnes SW, Walker JR, Vance J,...

SST Peer Reviewed

Predicting in vivo drug interactions from in vitro drug discovery data.

In vitro screening for drugs that inhibit cytochrome P450 enzymes is well established as a means for predicting potential metabolism-mediated drug interactions in vivo. Given that these predictions are based on enzyme kinetic parameters observed from...

Authors: Wienkers LC1, Heath TG

Efficient CRISPR/Cas9-mediated editing of trinucleotide repeat expansion in myotonic dystrophy patient-derived iPS and myogenic cells.

CRISPR/Cas9 is an attractive platform to potentially correct dominant genetic diseases by gene editing with unprecedented precision. In the current proof-of-principle study, we explored the use of CRISPR/Cas9 for gene-editing in myotonic dystrophy...

Authors: Dastidar S, Ardui S, Singh K, Majumdar D, Nair N, Fu Y, Reyon D, Samara E, Gerli MFM, Klein AF, De...

SST Peer Reviewed

Ribonucleoprotein Transfection for CRISPR/Cas9-Mediated Gene Knockout in Primary T Cells.

CRISPR/Cas9 has enabled the rapid and efficient generation of gene knockouts across various cell types of several species. T cells are central players in adaptive immune responses. Gene editing in primary T cells not only represents a valuable...

Authors: Oh SA, Seki A, Rutz S.

SST Peer Reviewed

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A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions.

ADME of biologics-what have we learned from small molecules?

HIF1a-dependent glycolysis promotes macrophage functional activities in protecting against bacterial and fungal infection

The utility of the minipig as an animal model in regulatory toxicology

In vitro gene expression analysis of hepatotoxic drugs in rat primary hepatocytes

Evaluation of an in vitro toxicogenetic mouse model for hepatotoxicity

Guide Swap enables genome-scale pooled CRISPR-Cas9 screening in human primary cells.

Predicting in vivo drug interactions from in vitro drug discovery data.

Efficient CRISPR/Cas9-mediated editing of trinucleotide repeat expansion in myotonic dystrophy patient-derived iPS and myogenic cells.

Ribonucleoprotein Transfection for CRISPR/Cas9-Mediated Gene Knockout in Primary T Cells.