Search Results

Simulation of human plasma concentration-time profiles of the partial glucokinase activator PF-04937319 and its disproportionate N-demethylated metabolite using humanized chimeric mice and semi-physiological pharmacokinetic modeling

The partial glucokinase activator N,N-dimethyl-5-((2-methyl-6-((5-methylpyrazin-2-yl)carbamoyl)benzofuran-4-yl)oxy)pyrimidine-2-carboxamide (PF-04937319) is biotransformed in humans to...

Authors: Kamimura H1,2, Ito S1, Chijiwa H3, Okuzono T3, Ishiguro T3, Yamamoto Y3, Nishinoaki S3, Ninomiya...

Pulsed Electromagnetic Field Regulates MicroRNA 21 Expression to Activate TGF-ß Signaling in Human Bone Marrow Stromal Cells to Enhance Osteoblast Differentiation.

Pulsed electromagnetic fields (PEMFs) have been documented to promote bone fracture healing in nonunions and increase lumbar spinal fusion rates. However, the molecular mechanisms by which PEMF stimulates differentiation of human bone marrow stromal...

Authors: Selvamurugan N1, He Z2, Rifkin D3, Dabovic B3, Partridge NC2

Impact of starting material (fresh versus cryopreserved marrow) on mesenchymal stem cell culture

BACKGROUND:Mesenchymal stem cells (MSCs) continue to be investigated in multiple clinical trials as potential therapy for different disorders. There is ongoing controversy surrounding the clinical use of cryopreserved versus fresh MSCs. However,...

Authors: Kaplan A1,2, Sackett K3, Sumstad D4, Kadidlo D4, McKenna DH3,4

Viability of primary cultured podocytes is associated with extracellular high glucose-dependent autophagy downregulation

Structural and functional impairment of podocytes plays an important role in the development of diabetic nephropathy, a chronic complication of diabetes mellitus and leading cause of renal failure requiring renal replacement therapy. Autophagy plays...

Authors: Audzeyenka I, Rogacka D, Piwkowska A, Angielski S, Jankowski M.

Gene amplification in mesenchymal stem cells and during differentiation towards adipocytes or osteoblasts.

Gene amplifications are an attribute of tumor cells and have for long time been overlooked in normal cells. A growing number of investigations describe gene amplifications in normal mammalian cells during development and differentiation. Possibly,...

Authors: Altmayer NC1, Galata V2, Warschburger N1, Keller A2, Meese E1, Fischer U1

Nucleofection-based gene targeting in human pre-B cells.

Electroporation is a powerful and convenient means for transfection of nonviral vectors into mammalian cells, providing an essential tool for numerous applications including gene targeting via homologous recombination. Recent evidence clearly...

Authors: Kurosawa A, Saito S, Mori M, Adachi N.

Clinical grade manufacturing of genetically modified, CAR-expressing NK-92 cells for the treatment of ErbB2-positive malignancies.

BACKGROUND: The NK-92/5.28.z cell line (also referred to as HER2.taNK) represents a stable, lentiviral-transduced clone of ErbB2 (HER2)-specific, second-generation CAR-expressing derivative of clinically applicable NK-92 cells. This study addresses...

Authors: Nowakowska P, Romanski A, Miller N, Odendahl M, Bonig H, Zhang C, Seifried E, Wels WS, Tonn T

SST Peer Reviewed

A biomaterials approach to influence stem cell fate in injectable cell-based therapies.

BACKGROUND:Numerous stem cell therapies use injection-based administration to deliver high-density cell preparations. However, cell retention rates as low as 1% have been observed within days of transplantation. This study investigated the effects of...

Authors: Amer MH1, Rose FRAJ1, Shakesheff KM1, White LJ2

Reprogramming Methods Do Not Affect Gene Expression Profile of Human Induced Pluripotent Stem Cells

Induced pluripotent stem cells (iPSCs) are pluripotent cells derived from adult somatic cells. After the pioneering work by Yamanaka, who first generated iPSCs by retroviral transduction of four reprogramming factors, several alternative methods to...

Authors: Marta Trevisan, Giovanna Desole, Giulia Costanzi, Enrico Lavezzo, Giorgio Palù, and Luisa Barzon*

SST Peer Reviewed

A Novel In Vitro Liver Cell Culture Flow System Allowing Long-Term Metabolism and Hepatotoxicity Studies

Introduction: Hepatotoxicity is a concern when developing new pharmaceutical compounds. Animal models do not accurately predict toxicity in humans due to species differences, for example, in compound metabolism. In this study, we present an...

Authors: Buesch S, Schroeder S, Bunger M, D'Souza T, Stosic M

SST Peer Reviewed

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Simulation of human plasma concentration-time profiles of the partial glucokinase activator PF-04937319 and its disproportionate N-demethylated metabolite using humanized chimeric mice and semi-physiological pharmacokinetic modeling

Pulsed Electromagnetic Field Regulates MicroRNA 21 Expression to Activate TGF-ß Signaling in Human Bone Marrow Stromal Cells to Enhance Osteoblast Differentiation.

Impact of starting material (fresh versus cryopreserved marrow) on mesenchymal stem cell culture

Viability of primary cultured podocytes is associated with extracellular high glucose-dependent autophagy downregulation

Gene amplification in mesenchymal stem cells and during differentiation towards adipocytes or osteoblasts.

Nucleofection-based gene targeting in human pre-B cells.

Clinical grade manufacturing of genetically modified, CAR-expressing NK-92 cells for the treatment of ErbB2-positive malignancies.

A biomaterials approach to influence stem cell fate in injectable cell-based therapies.

Reprogramming Methods Do Not Affect Gene Expression Profile of Human Induced Pluripotent Stem Cells

A Novel In Vitro Liver Cell Culture Flow System Allowing Long-Term Metabolism and Hepatotoxicity Studies