Search Results

Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells.

Genetic diseases of blood cells are prime candidates for treatment through ex vivo gene editing of CD34+ hematopoietic stem/progenitor cells (HSPCs), and a variety of technologies have been proposed to treat these disorders. Sickle cell disease (SCD)...

Authors: DeWitt MA, Magis W, Bray NL, Wang T, Berman JR, Urbinati F, Heo SJ, Mitros T, Muñoz DP, Boffelli D,...

SST Peer Reviewed

Evaluation of various static and dynamic modeling methods to predict clinical CYP3A induction using in vitro CYP3A4 mRNA induction data

Several drug-drug interaction (DDI) prediction models were evaluated for their ability to identify drugs with cytochrome P450 (CYP)3A induction liability based on in vitro mRNA data. The drug interaction magnitudes of CYP3A substrates from 28...

Authors: Einolf HJ1, Chen L2, Fahmi OA3, Gibson CR4, Obach RS3, Shebley M5, Silva J6, Sinz MW7, Unadkat JD8,...

Rhesus iPSC Safe Harbor Gene-Editing Platform for Stable Expression of Transgenes in Differentiated Cells of All Germ Layers.

Nonhuman primate (NHP) induced pluripotent stem cells (iPSCs) offer the opportunity to investigate the safety, feasibility, and efficacy of proposed iPSC-derived cellular delivery in clinically relevant in vivo models. However, there is need for...

Authors: Hong SG, Yada RC, Choi K, Carpentier A, Liang TJ, Merling RK, Sweeney CL, Malech HL, Jung M, Corat...

SST Peer Reviewed

CYP2C19-mediated (S)-mephenytoin 4'-hydroxylation assayed by high-performance liquid chromatography with radiometric detection.

A reverse-phase, high-performance liquid chromatography method is described for the quantification of 4'-hydroxymephenytoin formed enzymatically from 14C-labeled (S)-mephenytoin following incubation with cDNA-expressed CYP2C19 or human liver...

Authors: Crespi CL1, Chang TK, Waxman DJ.

Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation

Assisted reproductive technologies in all mammals are critically dependent on the quality of the oocytes used to produce embryos. For reasons not fully clear, oocytes matured in vitro tend to be much less competent to become fertilized, advance to...

Authors: Yuan Y, Spate LD, Redel BK, Tian Y, Zhou, Prather R, Roberts RM

ADME of biologics-what have we learned from small molecules?

Thorough characterization and in-depth understanding of absorption, distribution, metabolism, and elimination (ADME) properties of a drug candidate have been well recognized as an important element in small molecule (SM) drug discovery and...

Authors: Prueksaritanont T1, Tang C.

Data on HO-1 and CD200 protein secretion during T-cells and mesenchymal stromal cells co-cultures.

In this Data in Brief, we have provided data describing the secretion profile of two main immunoregulatory proteins, heme oxygenase-1 (HO-1) and CD200, from bone marrow (BM), Wharton?s Jelly (WJ) or adipose tissue (AT) mesenchymal stromal cells...

Authors: Fayyad-Kazan M, Fayyad-Kazan H, Merimi M, Meuleman N, Bron D, Lagneaux L, Najar M

The utility of the minipig as an animal model in regulatory toxicology

In this article we review the value and utility of the minipig as an animal model in regulatory toxicity testing. Our review is based on detailed consideration of the comparative biology of the minipig, and of the practical features of toxicity...

Authors: Bode G1, Clausing P, Gervais F, Loegsted J, Luft J, Nogues V, Sims J; Steering Group of the RETHINK...

In vitro gene expression analysis of hepatotoxic drugs in rat primary hepatocytes

The study examined the feasibility of screening for hepatotoxicity by an in vitro gene expression analysis using rat primary hepatocytes and Affymetrix Rat Toxicology U34 arrays. Hepatocytes were exposed for 6 or 24 h to eight drugs, with different...

Authors: Suzuki H1, Inoue T, Matsushita T, Kobayashi K, Horii I, Hirabayashi Y, Inoue T.

Co-culture of outgrowth endothelial cells with human mesenchymal stem cells in silk fibroin hydrogels promotes angiogenesis.

Sufficient vascularization of the implant construct is required for tissue regeneration to ensure the supply of oxygen and nutrients. In our previous work, we established sonication-induced silk fibroin hydrogel to load neural stem cells for brain...

Authors: Sun W, Motta A, Shi Y, Seekamp A, Schmidt H, Gorb SN, Migliaresi C, Fuchs S.

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