Search Results

Engineering a vascularised 3D in vitro model of cancer progression

The hallmark of tumours is the ability of cancerous cells to promote vascular growth, to disseminate and invade to distant organs. The metastatic process is heavily influenced by the extracellular matrix (ECM) density and composition of the...

Authors: Tarig Magdeldin, Víctor López-Dávila, Judith Pape, Grant W. W. Cameron, Mark Emberton, Marilena...

A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors.

Host proteins are essential for HIV entry and replication and can be important nonviral therapeutic targets. Large-scale RNA interference (RNAi)-based screens have identified nearly a thousand candidate host factors, but there is little agreement...

Authors: Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, Monel B, Schumann K, Yu H, Krupzcak...

SST Peer Reviewed

Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing.

Gene silencing is instrumental to interrogate gene function and holds promise for therapeutic applications. Here, we repurpose the endogenous retroviruses' silencing machinery of embryonic stem cells to stably silence three highly expressed genes in...

Authors: Amabile A, Migliara A, Capasso P, Biffi M, Cittaro D, Naldini L, Lombardo A.

SST Peer Reviewed

Acetaldehyde accelerates HCV-induced impairment of innate immunity by suppressing methylation reactions in liver cells

Alcohol exposure worsens the course and outcomes of hepatitis C virus (HCV) infection. Activation of protective antiviral genes is induced by IFN-a signaling, which is altered in liver cells by either HCV or ethanol exposure. However, the mechanisms...

Authors: Murali Ganesan,1,2 Jinjin Zhang,3 Tatiana Bronich,3 Larisa I. Poluektova,2,4 Terrence M. Donohue,...

Marker-free coselection for CRISPR-driven genome editing in human cells.

Targeted genome editing enables the creation of bona fide cellular models for biological research and may be applied to human cell-based therapies. Therefore, broadly applicable and versatile methods for increasing its efficacy in cell populations...

Authors: Agudelo D, Duringer A, Bozoyan L, Huard CC, Carter S, Loehr J, Synodinou D, Drouin M, Salsman J,...

SST Peer Reviewed

Characterization of the interplay between DNA repair and CRISPR/Cas9-induced DNA lesions at an endogenous locus.

The CRISPR-Cas9 system provides a versatile toolkit for genome engineering that can introduce various DNA lesions at specific genomic locations. However, a better understanding of the nature of these lesions and the repair pathways engaged is...

Authors: Bothmer A, Phadke T, Barrera LA, Margulies CM, Lee CS, Buquicchio F, Moss S, Abdulkerim HS, Selleck...

3D Cultivation Techniques for Primary Human Hepatocytes.

One of the main challenges in drug development is the prediction of in vivo toxicity based on in vitro data. The standard cultivation system for primary human hepatocytes is based on monolayer cultures, even if it is known that these conditions...

Authors: Bachmann A1, Moll M2, Gottwald E3, Nies C4, Zantl R5, Wagner H6, Burkhardt B7, Sánchez JJ8, Ladurner...

High-Throughput Platform for Identifying Molecular Factors Involved in Phenotypic Stabilization of Primary Human Hepatocytes In Vitro.

Liver disease is a leading cause of morbidity worldwide and treatment options are limited, with organ transplantation being the only form of definitive management. Cell-based therapies have long held promise as alternatives to whole-organ...

Authors: Shan J1, Logan DJ2, Root DE3, Carpenter AE3, Bhatia SN4

CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells.

T cells engineered with chimeric antigen receptor (CAR) have been successfully applied to treat advanced refractory B cell malignancy. However, many challenges remain in extending its application toward the treatment of solid tumors. The...

Authors: Zhang Y, Zhang X, Cheng C, Mu W, Liu X, Li N, Wei X, Liu X, Xia C, Wang H.

SST Peer Reviewed

Culture of bovine hepatocytes: a non-perfusion technique for cell isolation

In this work we have studied the isolation and culture of mature bovine hepatocytes on plastic dishes without exogenous matrix. The liver has been disaggregated in a collagenase solution instead of undergoing a perfusion step. After a few days in...

Authors: Spotorno VG1, Hidalgo A, Barbich M, Lorenti A, Zabal O.

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Research Area

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Topic

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Origin

Engineering a vascularised 3D in vitro model of cancer progression

A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors.

Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing.

Acetaldehyde accelerates HCV-induced impairment of innate immunity by suppressing methylation reactions in liver cells

Marker-free coselection for CRISPR-driven genome editing in human cells.

Characterization of the interplay between DNA repair and CRISPR/Cas9-induced DNA lesions at an endogenous locus.

3D Cultivation Techniques for Primary Human Hepatocytes.

High-Throughput Platform for Identifying Molecular Factors Involved in Phenotypic Stabilization of Primary Human Hepatocytes In Vitro.

CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells.

Culture of bovine hepatocytes: a non-perfusion technique for cell isolation